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Preparative Nondenaturing Gel Electrophoresis Used in the Purification of an Esterase Involved in Insecticide Resistance, Rev C

r> As shown in Table 1, there was an 87.0% recovery of enzyme activity applied to the Model 491 prep cell. The protein appeared to be homogeneous as determined by SDS-PAGE (Figure 3). In addition, several other esterases that appear as electrophoretic allozymes on native PAGE also have been separated by the Model 491 prep cell under the conditions described above. The purified enzymes have been characterized (by kinetics) with various insecticides to determine that qualitative differences exist in mosquito populations from different geographic locations.


References
Ketterman AJ et al., Purification and characterization of a carboxylesterase involved in insecticide resistance from the mosquito Culex quinquefasciatus, Biochem J 287, 355360 (1992)

Raymond M et al., Worldwide migration of amplified insecticide resistance genes in mosquitoes, Nature 350, 151153 (1991)


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