Navigation Links
Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix

Paul Burr, Wellcome Clinical Scholar, Department of Veterinary Pathology, University of Glasgow, Bearsden Rd, Glasgow. G61 1QH.


Introduction
A method that can be conveniently used to prepare DNA from a wide range of tissues for analysis in the Polymerase Chain Reaction is described. The method is both quick and simple, with a low risk of inadvertent contamination of samples due to the reduced number of manipulations required compared to other DNA extraction methods.


Methods
Canine tissues (tri-geminal ganglion, lumbo-sacral ganglion, brain, mesenteric lymph node, sub-mandibular lymph node, tonsil, sub-mandibular salivary gland, parotid salivary gland, spleen, liver and kidney) were collected from a number of dogs during routine post mortem. All tissues were collected within 2 hours of death and frozen at -70 C until processed. Great care was taken during preparation to avoid problems of both environmental and cross contamination.

1. Cut a small (3 mm3) piece of tissue on a fresh glass slide using a fresh scalpel blade. Macerate slightly with the blade and place in a sterile 1.5 ml screw cap Eppendorf tube.

2. Add 1 ml of 1x Trypsin/EDTA (Gibco BRL). Incubate at room temperature for 15-30 minutes, shaking occasionally.

3. Spin down (13K, 2-3 minutes) to pellet tissue, and carefully remove all the supernatant.

4. Add 250 l of InstaGene matrix (Bio-Rad Laboratories), and mix well. Incubate at 56 C for 30 minutes, mixing occasionally.

5. Vortex tube thoroughly for 10 seconds, place in boiling water bath or 100 C heat block for 8 minutes.

6. Vortex thoroughly for 10 seconds, spin at 13K for 2-3 minutes.
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Sample Preparation for Microinjection
2. High Pure PCR Template Preparation Kit
3. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
4. Dicer vs. RNase III for Preparation of siRNA Cocktails
5. Genomic DNA Preparation from RNAlater Preserved Tissues
6. Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities
7. Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A
8. High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation
9. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
10. Generate High-Quality, Directional Plasmid cDNA Libraries
11. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
Post Your Comments:
(Date:4/30/2015)... 2015 /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ), ... it has commenced an underwritten public offering of its ... underwriters a 30-day option to purchase up to an ... offered in the public offering. The offering is subject ... as to whether or when the offering may be ...
(Date:4/30/2015)... (PRWEB) April 30, 2015 Biodata, ... notebook with integrated protocols and specimen management systems, ... solutions and consulting services for life science research, ... of Labguru’s newest feature: chemical structure and reaction ... features for biologists, bio-chemists, and more, the integration ...
(Date:4/30/2015)... , April 30, 2015 ... Leading Global Biotechnology Company   Shire ... for the three months to March 31, 2015. ... sales $1,423 million +9% +13% Total revenues $1,488 million ... +19% US GAAP operating income from continuing operations $475 ...
(Date:4/30/2015)... CA (PRWEB) April 30, 2015 SureClinical, ... that Julie I. Morris has joined SureClinical’s executive team ... an experienced biotech and technology executive with over 25 ... Fortune 500 companies. , Ms. Morris joins SureClinical ... biotech company. She was CFO of MBI from its ...
Breaking Biology Technology:BioAmber Inc. Announces Public Offering Of Common Stock 2BioAmber Inc. Announces Public Offering Of Common Stock 3Labguru Integrates ChemAxon's Marvin JS, to Enhance Functionality for Chemists 2Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 2Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 3Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 4Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 5Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 6Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 7Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 8Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 9Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 10Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 11Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 12Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 13Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 14Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 15Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 16Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 17Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 18Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 19Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 20Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 21Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 22Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 23Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 24Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 25Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 26Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 27Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 28Shire Delivers Strong Revenue Growth and Cash Generation; 20% Increase in Non GAAP Earnings per ADS 29Julie I. Morris Joins SureClinical as Chief Financial Officer 2
... additional capacity in Iowa and Illinois to meet diesel market demand. ... ... Renewable Energy Group® today announced it has entered into agreements to ... a 30 MGY facility in Wall Lake, Iowa; Central Iowa Energy, ...
... Four prestigious universities have joined Bio Career Center, the ... science professionals. UCLA , Brown , NYU ... 15 other major universities.The jobs board, www.biocareercenter.com ... there are hundreds of life science career sites on the ...
... registered by the Department of Veterans Affairs and the ... complaints with the U.S. Department of Veterans Affairs, and ... by the New York State Department of Health (NYSDOH)."Fortunately, ... enterprises, and I am grateful for them," said Gerard ...
Cached Biology Technology:REG Signs Agreement with Three Biodiesel Plants to Consolidate 2REG Signs Agreement with Three Biodiesel Plants to Consolidate 3REG Signs Agreement with Three Biodiesel Plants to Consolidate 4REG Signs Agreement with Three Biodiesel Plants to Consolidate 5REG Signs Agreement with Three Biodiesel Plants to Consolidate 6REG Signs Agreement with Three Biodiesel Plants to Consolidate 7Four Major Universities Join Nation's Leading Postgrad Bio Jobs Board 2Veteran-Owned Biotech Startup Company Charges Health Agency (NYSDOH) with Blackballing its Initiatives 2Veteran-Owned Biotech Startup Company Charges Health Agency (NYSDOH) with Blackballing its Initiatives 3
(Date:4/20/2015)... The announcement comes as demand ... Management (GRM), Ireland,s foremost records management ... built up an impressive track record of clients within the ... the records management sector in Dubai . ... staffbase and employ a further eight staff members at its ...
(Date:4/14/2015)... 14, 2015  HYPR Corp. today announced it ... ) Alliance tm , an industry consortium transforming ... to share technology and collaborate to deliver open ... more secure and private, and easier to use. ... that protects sensitive user information and eliminates the ...
(Date:4/13/2015)... , April 13, 2015 ... TechSci Research, "Global Biometrics Market Forecast & Opportunities, 2020", ... a CAGR of around 14% till 2020. The ... for large scale implementation and review of biometric ... launch of new products with greater efficiency, are ...
Breaking Biology News(10 mins):Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3HYPR Corp. Joins the FIDO Alliance 2HYPR Corp. Joins the FIDO Alliance 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2
... S. Serianni is providing new insights that could have ... points out that biological compounds known as dicarbonyl sugars ... breakdown of the simple sugar, glucose. The formation of ... concentrations in the blood and plasma of diabetics are ...
... Dec. 12, 2012 Wake Forest Baptist Medical Center ... its academic and clinical research and discoveries into marketable ... enterprise, Wake Forest Innovations, is being led by Chief ... who is also President of the Piedmont Triad ...
... humans and 200 Asian elephants every year. One of the ... elephants into ranges away from humans, often into national parks. ... elephants raiding crops, breaking into homes and injuring or killing ... published Dec. 7 in PLOS ONE by the ...
Cached Biology News:Notre Dame research may have important implications for combating diabetes 2Wake Forest Baptist Medical Center Launches Wake Forest Innovations 2Wake Forest Baptist Medical Center Launches Wake Forest Innovations 3Relocating elephants fails to decrease human-wildlife conflict 2
... Variable Speed Rocker, with 12" x 14" table; ... x 14" table; 220V. The VSR-50 Variable ... for any lab. The VSR-50 provides a rhythmic ... a gentle to vigourous movement. This makes it ...
...
...
...
Biology Products: