Navigation Links
Precursor miRNAs for Successful miRNA Functional Studies

Pre-miR miRNA Precursor Molecules
miRNA target site identification and validation
Screening for miRNAs that regulate target gene expression
Screening for miRNAs that affect a cellular process


MicroRNAs (miRNAs) are small, regulatory RNAs that are expressed in animals and plants and affect the translation or stability of target mRNAs. The 17-24 nt, single-stranded (ss) miRNAs are derived from longer, primary transcripts termed "pri-miRNAs" [1]. The pri-miRNAs, which can be more than 1000 nt in length, contain an RNA hairpin in which one of the two strands includes the mature miRNA [1]. The hairpin, which typically comprises 60-120 nt, is cleaved from the pri-miRNA in the nucleus by the double-strand-specific ribonuclease, Drosha [1]. The resulting precursor miRNA, or "pre-miRNA," is transported to the cytoplasm via a process that involves Exportin-5 [2-4]. The pre-miRNA is further cleaved by Dicer [5] to generate a short, partially double-stranded (ds) RNA in which one strand is the mature miRNA. The mature miRNA is taken up by a protein complex that is similar, if not identical, to the RNA Induced Silencing Complex (RISC) that supports RNA interference (RNAi) [6], and miRNA-bound complex functions to regulate translation.

miRNA functional analyses can be carried out using tools that are similar to those used to analyze protein encoding genes. Up-regulation of the miRNAs
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11

Related biology technology :

1. Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform
2. Keys to Successful Densitometry
3. Successful PCR amplification and subcloning of a GC-rich DNA fragment
4. General Considerations for Successful Transfection Experiments
5. Designing a Successful qRT-PCR Experiment
6. Recommendations for Successful siRNA Library Screens
7. Setting up Successful siRNA Library Screens
8. Successful stabilization of gene expression profiles
9. Detecting miRNA & siRNA
10. miRNA Expression in White Blood Cells
11. Isolate It All: siRNA miRNA Total RNA Native Protein
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Precursor miRNAs for Successful miRNA Functional Studies

(Date:3/27/2015)... Mich. , March 27, 2015  Neogen Corporation ... LLC, has adopted a prearranged trading plan in accordance ... Herbert , Chairman and CEO of Neogen Corporation, is ... of Neogen Corporation common stock. Herbert does ... disclosure was appropriate. The filing is only for a ...
(Date:3/27/2015)... March 27, 2015 WuXi PharmaTech (Cayman) ... R&D capability and technology platform company serving the ... today that an Investigational New Drug (IND) application ... rheumatoid arthritis has been accepted for review by ... In September 2012, MedImmune, the global ...
(Date:3/26/2015)... PRC Clinical, a Silicon-Valley based ... and executive appointments effective immediately. , Mike Catelani will ... member of the Board of Directors. Daniel Head is ... Dowden will assume the position of Senior Director of ... Officer (COO), has been appointed to the position of ...
(Date:3/26/2015)... and CINCINNATI , March ... (LSE: SHP, NASDAQ: SHPG ) and Cincinnati ... broad research collaboration for rare diseases. The goal of ... therapies to treat rare diseases with high unmet ... with Cincinnati Children,s research expertise. As a nationally ...
Breaking Biology Technology:Neogen CEO adopts 10b5-1 Trading Plan 2Investigational New Drug Application for WuXi MedImmune's Monoclonal Antibody Accepted for Review by CFDA 2Investigational New Drug Application for WuXi MedImmune's Monoclonal Antibody Accepted for Review by CFDA 3PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 2PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 3Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 2Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 3Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 4Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 5
... 27 Mediatech, Inc. is settling,into their brand ... land,in Prince William County. Right down the road ... joins other,biotechnology and technology companies, including ATCC in ... The brand new manufacturing plant has been ...
... Amended the Design of the Ofatumumab Pivotal Study in, ... ... 81, COPENHAGEN, September 27 Genmab A/S (OMX:,GEN) announced ... study,of ofatumumab (HuMax-CD20(R)) in rituximab refractory follicular,non-Hodgkin,s lymphoma (NHL) to ...
... for October, JACKSONVILLE, Fla., Sept. 27 ... announced today that its,performance/vitality beverage product, AQUISS(TM), will ... to the Oregon border. 7-Eleven,plans a launch of ... sampling campaign to acquaint 7-Eleven,s customers with the,technology ...
Cached Biology Technology:Mediatech Moves to Prince William County Innovation Park 2Genmab Amends Ofatumumab Pivotal Study in NHL to Single Arm Study 2Genmab Amends Ofatumumab Pivotal Study in NHL to Single Arm Study 3Phlo Affiliate Reports AQUISS to be Sold in 450 7-Eleven Stores 2
(Date:3/16/2015)... 2015 Between 16 and ... groundbreaking innovations in biometric identification at the CeBIT ...      (Photo: http://photos.prnewswire.com/prnh/20150316/735333 ) ... well at the forefront: the company,s all new ... Germany . BiometricGate is the ...
(Date:3/12/2015)... WINSTON-SALEM, N.C. , March 12, 2015 ... afternoon at Brenner Children,s Hospital, part of Wake Forest ... an outreach initiative for the 2015 ACC Men,s Basketball ... taking pictures with them and getting their autographs. ... Wake Forest Baptist. It is the only children,s hospital ...
(Date:3/12/2015)... 2015 WHEN:Tuesday, March 17, 2015 at 11:00 ... . SPEAKERS: , Frost & Sullivan Measurement & ... Biometric companies must plan for ... different markets and industries. This is especially true ... Join Frost & Sullivan,s upcoming ...
Breaking Biology News(10 mins):DERMALOG Presents its Latest Biometric Innovations at the Hannover CeBIT Exhibition, 16 - 20 March 2015 2DERMALOG Presents its Latest Biometric Innovations at the Hannover CeBIT Exhibition, 16 - 20 March 2015 3Biometrics Key to Future Growth in Healthcare, Retail and Financial Markets 2Biometrics Key to Future Growth in Healthcare, Retail and Financial Markets 3
... September 11, 2012 BiOptix announced continued support of the ... 2012, a leading life sciences showcase event in ... "BiOptix continues to believe in the goals and ... Rick Whitcomb, President and CEO of BiOptix.   "As ...
... a crow and it,ll remember you for years. Crows and ... with negative, as well as positive, feelings. The way the ... also appear to share, according to new research being published ... together are not unlike those that work together in mammals, ...
... found a way to prevent inflammation and bone loss ... of the enzyme GSK3b, which plays an important role ... of UofL School of Dentistry researcher David Scott, PhD, ... journal Molecular Medicine . The finding not only ...
Cached Biology News:BiOptix Participates in Leading Life Sciences Event BioWest 2012 2Crows react to threats in human-like way 2Crows react to threats in human-like way 3
... Affinity Life Sciences can provide custom microplate coating using ... washes a variety of plate formats. We can also accommodate ... volume range of 0.5-320 L):, , ... 96 well microplate, ...
...
... first biological reagent that eliminates ... not just by inhibiting growth. ... agent that can be used ... The cytotoxicity of Mynox is ...
Mynox is intended for research use only. Mynox is used for the elimination of Mycoplasma and Acholeplasma in cell and virus cultures, and other biologicals....
Biology Products: