Navigation Links
Practical Aspects of Evaluation of Chromatographic Data in Size Exclusion Chromatography


Ivan Vin, DataApex Ltd., Praha, Czech Republic www.dataapex.com
e-mail: vins@dataapex.com
Duan Berek, Polymer Institute SAV, Bratislava, Slovak Republic


Introduction
Determination of MW distribution of polymers by the liquid chromatographic methods with concentration or mass selective detectors is a simple, affordable solution for process quality control or research and development of synthetic and natural polymers. Absolute methods with molecular weight and viscosity detectors are more demanding and not always affordable.

Using this methods, precision and accuracy of calculated values is of prime importance. GPC/SEC is presently the most common method for determination of molar masses and molar mass distributions of synthetic polymers. It is known for its high intra-laboratory repeatability (precision) of the molar mass data, often better than +/- 5%. At the same time, GPC suffers from a rather low inter-laboratory reproducibility (accuracy). The molar mass data scatter is +/- 10-20% at best and often may reach even several hundreds of percent. In this note, we will discuss the potential sources of errors during GPC data.

The factors influencing the GPC data, like column quality (resolution, efficiency), sample preparation, sample concentration and volume, extracolumn dispersion, sample/solvent/column interactions, detector sensitivity and linearity, instrument flow rate and temperature stability are outside scope of this note and are widely discussed elsewhere.


Factors influencing data processing in GPC/SEC
The data processing in chromatography software consists of data acquisition, integration, calibration and calculation of the results. The possible problems in those areas will be discussed.


Data Acquisition
Analog signal
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Acrylamide Polymerization A Practical Approach
2. Practical Applications of HyperDSC in a Pharmaceutical Laboratory
3. Evaluation of FlashPlate in a Helicase Assay
4. Accurate Evaluation of the Finnigan Surveyor Autosampler Performance using a Deferred Standard
5. Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples
6. Use of On-line Size Exclusion Chromatography (SEC) for Sample Clean-up in LC/MS Analyses
7. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
8. Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns
9. Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A
10. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
11. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
Post Your Comments:
(Date:8/19/2014)... BURNIE, Md. , Aug. 19, 2014 ... clinical research industry, recently announced the formation of the ... pleased to announce the addition of Dr. William ... Photo - http://photos.prnewswire.com/prnh/20140818/136957 The ... across a variety of therapeutic areas and set strategic ...
(Date:8/19/2014)... , Aug. 19, 2014   Synthetic Biologics, ... developing novel anti-infective biologic and drug programs targeting ... announced today that its novel C. difficile ... 54 th Interscience Conference on Antimicrobial Agents ... Washington D.C. Synthetic Biologics, ...
(Date:8/19/2014)... August 19, 2014 Sales Horizons, a ... its online sales skills training course to ... , MedTech clinical staff spend the majority of their ... standalone medical center, or physician practice – providing support ... why stop there? Sales Horizons believes clinical staff are ...
(Date:8/18/2014)... new therapy developed by researchers at the University ... and Columbia University Medical Center (CUMC) may help ... treatment. , The researchers demonstrated in a ... selectively inhibit blood vessel re-narrowing and simultaneously promote ... balloon catheter to open narrowed or blocked arteries. ...
Breaking Biology Technology:CSSi Appoints Dr. William E. Gannon, Jr. to Newly Formed Medical and Clinical Advisory Board 2CSSi Appoints Dr. William E. Gannon, Jr. to Newly Formed Medical and Clinical Advisory Board 3Synthetic Biologics Announces Late-Breaking Poster Presentation for C. difficile Program at 54th ICAAC 2Synthetic Biologics Announces Late-Breaking Poster Presentation for C. difficile Program at 54th ICAAC 3Synthetic Biologics Announces Late-Breaking Poster Presentation for C. difficile Program at 54th ICAAC 4Sales Horizons Launches a New Online Sales Training Course for MedTech Clinical Staff to Develop Their Sales Skills 2Sales Horizons Launches a New Online Sales Training Course for MedTech Clinical Staff to Develop Their Sales Skills 3Selective therapy may improve artery repair after interventional cardiovascular procedures 2Selective therapy may improve artery repair after interventional cardiovascular procedures 3
... July 23 /PRNewswire-FirstCall/ - Roche,(SWX: ROG) and ARIUS ... have signed a definitive agreement for Roche to ... of approximately C$191 million. ARIUS is,the developer of ... and selects antibodies based on their functional,ability to ...
... ALTO, Calif., July 22 CV Therapeutics,Inc. (Nasdaq: ... quarter,financial results for 2008 after the market closes on ... call at 5:00 p.m. EDT,2:00 p.m. PDT, on the ... log on to the Company,s website at, http://www.cvt.com ...
... N.J., July 22 The Board of,Directors of BD ... declared a,quarterly dividend of 28.5 cents per common share. ... holders of record on September 9, 2008.,The indicated annual ... BD is a leading global medical technology company ...
Cached Biology Technology:Roche starts acquisition process of ARIUS to gain access to new screening platform for antibody therapeutics 2Roche starts acquisition process of ARIUS to gain access to new screening platform for antibody therapeutics 3Roche starts acquisition process of ARIUS to gain access to new screening platform for antibody therapeutics 4CV Therapeutics to Announce 2008 Second Quarter Financial Results on Thursday, July 31, 2008 2
(Date:8/19/2014)... German . ... a large amount of electricity is to be produced with ... to be stored during productive periods so that these fluctuations ... from adequate for the purpose. Science and industry are therefore ... battery systems that used to be too expensive or unsophisticated ...
(Date:8/19/2014)... lemur. It,s not an African Bush Baby or even a ... downright "cool" primate from Southeast Asia. , "It,s really not ... Brown, curator-in-charge at the University of Kansas, Biodiversity Institute. "A ... body; a long tail with a furry tuft at the ... a bit like the disks on the digits of tree ...
(Date:8/19/2014)... New measurements from fish purchased at retail seafood ... to which mislabeling can expose consumers to unexpectedly ... Fishery stock "substitutions"which falsely present a fish of ... originare the most dangerous mislabeling offense, according to ... scientists. , "Accurate labeling of seafood is essential ...
Breaking Biology News(10 mins):Asian inventions dominate energy storage systems 2Asian inventions dominate energy storage systems 3Philippine tarsier gets boost from Kansas research, and genetic proof of a new variety 2Philippine tarsier gets boost from Kansas research, and genetic proof of a new variety 3Seafood substitutions can expose consumers to unexpectedly high mercury 2Seafood substitutions can expose consumers to unexpectedly high mercury 3
... Canadian researcher working in the U.K. says doctors, authors ... claiming that hyperactivity as we understand it today has ... that notion wrong, it misleads patients, their parents and ... finishing up his PhD at the Centre for Medical ...
... embarrassing, but a new study suggests that it might ... interviewed more than three thousand men - all pairs ... - about the first time they had sex. Many ... from erectile dysfunction and premature ejaculation at their first ...
... for proposals, projects focus on either the development of ... and genomics. The Swiss National Science Foundation (SNSF) approved ... 47 research groups from both Swiss Federal Institutes of ... from the Universities of Basel, Lausanne, Geneva and Zurich. ...
Cached Biology News:History of hyperactivity off-base, says researcher 2Swiss Initiative in Systems Biology launches new projects 2
... This DuoSet ELISA Development kit contains the ... sandwich ELISAs to measure natural and recombinant ... and serum. Each kit contains sufficient materials ... plates, provided that the following conditions are ...
Blimp-1 (H-150)...
Mouse Galectin-1 Affinity Purified Polyclonal Ab...
PSGL-1 (215)...
Biology Products: