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PolyFect Transfection Reagent

ntrast to many liposomal reagents, PolyFect Reagent enables transfection in the presence of serum without lowering of transfection efficiency.

High Transfection Efficiencies



Comparison of transfection efficiencies for PolyFect Reagent and two lipid-based reagents. Cells cultured in 6-well plates were transfected with a beta-galactosidasereporter plasmid. The appropriate protocol was used for transfection with PolyFect Reagent. Optimized protocols were used for transfection with Reagents L2 and F; these were: 10 l Reagent L2 with 3 g DNA and 15 l Reagent F with 2 g DNA for CHO cells, and 10 l Reagent L2 with 2 g DNA and 7 l Reagent F with 3 g DNA for 293 cells. All transfections were performed in triplicate. Two independent transfection experiments are shown for 293 cells.

PolyFect Reagent with HeLa Cells



Expression of A -galactosidase and B green fluorescent protein (GFP) in HeLa cells. Cells were cotransfected in 6-well plates with -galactosidase and GFP-reporter plasmids using PolyFect Transfection Reagent and the HeLa cell protocol. Expression was visualized by X-gal staining or fluorescence microscopy 2 days post-transfection.

High-throughput transfection

The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using PolyFect Reagent requires minimal handling and removal of transfection complexes is not required, making this reag
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