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PolyFect Transfection Reagent

structure. The upper right section of the illustration shows naked DNA, the lower section shows the interaction between dendrimers and DNA inside the complex, and the upper left section shows the final complete coverage of DNA within the complex.

Procedure

PolyFect Transfection Reagent is provided as a ready-to-use solution. The transfection procedure is fast and simple just add PolyFect Reagent to the DNA solution, mix, incubate for 510 minutes, add growth medium (which can contain serum and antibiotics), and pipet the PolyFectDNA complexes onto the cells. The cells are then incubated for gene expression. No post-transfection removal of complexes or medium change/addition is necessary, making the procedure fast and easy.




Applications


PolyFect Reagent has been specifically developed for fast and easy transfection of COS-7, NIH/3T3, HeLa, 293, and CHO cells. Optimized, cell-specific protocols for transfection of these cell lines in different cell-culture formats are provided in the PolyFect Transfection Reagent Handbook. In addition, optimized protocols for transfection of HeLa-S3 cells are available from QIAGEN Technical Services and the Transfection Tools web site (www.qiagen.com/transfectiontools/).

PolyFect Reagent consistently delivers high transfection efficiencies (see figures "High Transfection Efficiencies" and "PolyFect Reagent with HeLa Cells"). In co
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