( ...)PolyFect Transfection Reagent

Optimized for transfection of COS-7, NIH/3T3, HeLa, 293, and CHO cells

Features and benefits

• Optimized protocols for COS-7, NIH/3T3, HeLa, 293, and CHO cells
  
• Fast procedure and easy handling
  
• No removal of transfection complexes required
  
• Transfection in the presence of serum
  
• High efficiency
• Economical price
  

Principle

PolyFect Transfection Reagent is a solution of specifically designed activated-dendrimers. The reagent consists of dendrimer molecules of a defined spherical architecture with branches radiating from a central core (1; see figure "Activated-Dendrimer Structure"). The branches terminate at charged amino groups, which can interact with negatively charged phosphate groups of nucleic acids. PolyFect Reagent assembles DNA into compact structures (see figure "PolyFectDNA Interaction"), that bind to the cell surface and are taken into the cell by nonspecific endocytosis. The reagent buffers the pH of the endosome, leading to pH inhibitition of endosomal nucleases, which ensures stability of PolyFectDNA complexes.

Activated-Dendrimer Structure

Schematic representation of an activated dendrimer (1). Note the highly branched structure.

PolyFectDNA Interaction
Model of the SuperFectDNA complex. SuperFect Reagent (purple balls) interacts with DNA (black) to form a ring-like (toroid-like)
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