Navigation Links
Pfu DNA Polymerase as a Hot Start Alternative

lified with AmpliTaq Gold DNA polymerase under the conditions used. For the 120-bp target (figure 2B), extraneous products were synthesized with Taq DNA polymerase but not with AmpliTaq Gold DNA polymerase. The addition of Taq DNA polymerase-specific antibody or the magnesium wax beads did not eliminate the synthesis of artifacts. Finally, with the 230-bp target (figure 2C), undesired PCR products were absent when amplifications were conducted with Taq2000 DNA polymerase in the presence of magnesium wax beads or with AmpliTaq Gold DNA polymerase; however, PCR product yield was significantly reduced in reactions using AmpliTaq Gold DNA polymerase (lanes 5-6). For the 230-bp target, the addition of Taq DNA polymerase-specific antibody did not reduce the synthesis of undesired products by Taq DNA polymerase.

In total, our results demonstrate the variability of hot start techniques in eliminating the synthesis of undesired products by Taq DNA polymerase. In contrast, Pfu DNA polymerase (in figure 2A, 2B and 2C, lanes 9 and 10) consistently generated only the desired PCR product, and yields of PCR products were comparable to those generated with the best Taq DNA polymerase plus hot start combinations (figure 2A, lanes 3, 4, 7 and 8; figure 2B, lanes 5, 6; figure 2C, lanes 7, 8). The relatively poor performance of hot start techniques for amplifying the 120-bp and 230-bp targets suggests that mispaired primer-extension reactions may be occurring at primer-annealing steps that take place after the first PCR cycle. Of course, current hot start techniques are ineffective after the initial denaturation step. The use of Pfu DNA polymerase,
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
3. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
4. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
5. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
6. High-Fidelity PCR with a Novel Polymerase Mixture
7. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
8. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
9. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
10. HotStarTaq DNA Polymerase
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Pfu DNA Polymerase Hot Start Alternative

(Date:7/1/2015)... Colo. and BANGALORE, India ... global genomic profiling company that uses next generation sequencing ... of Scott A. Storrer as chief executive ... CEO, he will be responsible for all strategy, business ... Chandru , who has served as Strand,s chairman and ...
(Date:7/1/2015)... ... ... A newly launched business plan contest will ask startup companies from around ... sciences R&D. The Pistoia Alliance President’s Startup Challenge 2015 will see small and medium ... expert mentorship from a senior industry figure drawn from the Pistoia Alliance membership. , ...
(Date:7/1/2015)... NEW YORK , July 1, 2015 /PRNewswire/ ... G ), a global leader in designing, ... the Aecus Innovation Award in recognition of their ... operational efficiencies in UCB,s global business services (GBS) ... acknowledge service providers and clients that work together ...
(Date:6/30/2015)... ... June 30, 2015 , ... Park Systems , world-leader ... research into new classes of Nanoscale Graphene-based materials poised to revolutionize industries such ... material of the 21st Century' by the researchers who were awarded the 2010 ...
Breaking Biology Technology:Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 2Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 3Pistoia Alliance Launches Startup Challenge 2Pistoia Alliance Launches Startup Challenge 3UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 2UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 3UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 4UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 5How Graphene–based Nanomaterials and Films Revolutionize Science Explained in July 9 Webinar Hosted by Park Systems 2How Graphene–based Nanomaterials and Films Revolutionize Science Explained in July 9 Webinar Hosted by Park Systems 3How Graphene–based Nanomaterials and Films Revolutionize Science Explained in July 9 Webinar Hosted by Park Systems 4
... his organization's ability to seamlessly blend business and technology. "In ... IT folks," he said. That collaborative spirit reaches all the ... the executive committee, which sets vision and strategic direction for ... of orders in the warehouses. , , Giannantonio ...
... , ,A. Things stall; nothing gets done. , ... , ,C. Young professionals shake their heads and start ... might be living in a Next Generation Community. Last year ... a Young Professionals Organization hired us. Unwilling to wait for ...
... Wis. Web addicts, take heart: A public-private ... wi-fi hotspot. , , AOL-SkyCable has been chosen ... wireless Internet service for the general public. The "Wireless ... (wi-fi) network covering Madison and the Dane County Regional ...
Cached Biology Technology:Frank Giannantonio blends IT and business for Lands' End 2Frank Giannantonio blends IT and business for Lands' End 3Frank Giannantonio blends IT and business for Lands' End 4Lead, follow or get out of the way 2Madison wi-fi plans move ahead 2
(Date:6/11/2015)... , June 11, 2015 Daon, a ... today that its IdentityX Mobile Authentication Platform v4.0 ... Online) Alliance is an industry consortium launched in ... for simpler, stronger authentication.  In order to receive ... a rigorous series of tests that measure compliance ...
(Date:6/9/2015)... 2015   MedNet Solutions , an innovative SaaS-based ... clinical research, will be providing demonstrations of its ... 2015 Drug Information Association (DIA) Annual Meeting in ... presentations will include previews of many exciting new enhancements ... iMedNet 2015 Feature 1 Release. ...
(Date:6/8/2015)...  Trovagene, Inc. (NASDAQ: TROV ), a developer of ... Precision Cancer Monitoring SM platform will be presented at ... San Diego, CA on June 11-12 ... Clinical Genomics and Cancer Therapy in Salt ... "We continue to advance our clinical programs and present ...
Breaking Biology News(10 mins):Daon's IdentityX Mobile Authentication Platform One of First Products to be Certified by FIDO Alliance 2MedNet To Provide Sneak Peeks Of Its iMedNet "2015 Feature 1 Release" At The DIA Annual Meeting 2MedNet To Provide Sneak Peeks Of Its iMedNet "2015 Feature 1 Release" At The DIA Annual Meeting 3Clinical Data Featuring Trovagene's Urine-Based Liquid Biopsy Platform to be Presented at Two Upcoming Medical Conferences 2Clinical Data Featuring Trovagene's Urine-Based Liquid Biopsy Platform to be Presented at Two Upcoming Medical Conferences 3Clinical Data Featuring Trovagene's Urine-Based Liquid Biopsy Platform to be Presented at Two Upcoming Medical Conferences 4
... generation hemophilia treatment ,Researchers have doubled the potency of ... research to be published in the July 26 edition ... for the treatment of hemophilia, the inherited blood disorder ... , In most cases, hemophilia is caused by a ...
... pilot study at the National Institute of Standards and ... Early Detection Research Network (EDRN), has validated the measurement ... an early indicator for certain types of cancer. Additional ... a DNA microarray "chip" could enable early detection of ...
... the developing mammalian embryo is one of the most ... and Takashi Hiiragi have new evidence to fuel the ... the researchers showed compelling evidence for the absence of ... are in contrast to those who feel that the ...
Cached Biology News:Redesigned protein accelerates blood clotting 2
Antibodies were affinity purified using epitopes specific to Alpha4 immobilized on solid support....
Collected from 8-12 week old sexually mature rats. Pricing: $100/unit for 1 - 10 units...
... are K-12 derivatives that have mutations in ... reductase (gor) genes, which greatly enhances disulfide ... shown that expression in Origami(DE3) yielded 10-fold ... even though overall expression levels were similar ...
... The GenoStat Inducible Expression System provides tightly ... mammalian cells and is capable of repeated ... is mediated by the highly specific interaction ... bipartite nuclear receptor complex. Gene expression levels ...
Biology Products: