Navigation Links
Pfu DNA Polymerase as a Hot Start Alternative

/sub>of Pfu DNA polymerase are not unusual when one considers the natural habitat from which it was derived. Pfu DNA polymerase was cloned from the hyperthermophilic archaeon Pyrococcus furiosus, which grows optimally at 100C in geothermal marine sediments.4 In contrast, Taq DNA polymerase was cloned from the thermophilic eubacterium Thermus aquaticus, which grows optimally at 70C in thermal springs.4

Activity vs. Temperature Profiles

Figure 1

In order to demonstrate the difference in activity vs. temperature profiles between Taq and Pfu DNA polymerases, the polymerase activity of the two enzymes was compared over a range of temperatures encountered in PCR amplifications. The activities of cloned Taq and cloned Pfu DNA polymerases were compared at equal unit concentrations at temperatures ranging from 15 to 100C. The percent of maximal polymerase activity is plotted as a function of temperature in figure 1. Taq DNA polymerase exhibited optimal (84%) polymerase activity between 60 and 74C and 27% to 70% activity between 40 and 50C. Pfu DNA polymerase, on the other hand, exhibited optimal activity between 70 and 77C and only 2% to 8% activity between 40 and 50C. No significant differences in activity vs. temperature profiles were observed between cloned and native DNA polymerases (data not shown). Polymerization by Pfu DNA polymerase decreased dramatically at temperatures above 75 to 80C, presumably because the primer-template duplex dissociates at elevated temperatures. The Tm of the 40-base primer used in the primer-extension assay was calculated to be approximately 92.6C.5


'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
3. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
4. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
5. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
6. High-Fidelity PCR with a Novel Polymerase Mixture
7. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
8. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
9. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
10. HotStarTaq DNA Polymerase
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Pfu DNA Polymerase Hot Start Alternative

(Date:3/26/2015)... , March 26, 2015 /PRNewswire/ - SQI Diagnostics ... announced that after dealing with certain matters, including the ... shareholders held on March 26, 2015 (the "Meeting") was ... April 8, 2015 at 11:00 a.m. ( Toronto ... has been changed from that previous disclosed. The Meeting ...
(Date:3/26/2015)... 2015 PAREXEL International Corporation (Nasdaq: ... research organization , announced today that the ... acquire all of the business assets of privately-owned ... of specialized pharmacovigilance services, based in Chandigarh, ... assessment, monitoring, and prevention of adverse effects ...
(Date:3/25/2015)... is one of the most attractive in the emerging ... a growing economy and increasing government investment in healthcare, ... Russian market is set to grow at twice the ... around 10-15% annually reaching an approximate market value of ... Green Cross provides total healthcare solutions that ...
(Date:3/25/2015)... , March 25, 2015  S&P Capital ... Factual Stock Report coverage on Abattis Bioceuticals ... ATTBF; CSE: ATT): is a specialty biotechnology company ... cultivating, licensing and marketing proprietary ingredients, bio-similar compounds, ... in North America . The ...
Breaking Biology Technology:SQI Diagnostics Inc. Announces Adjournment of Meeting 2PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 2PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 3PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 4S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 2S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 3S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 4S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 5
... three more days before I can share the details of the ... have to trust me that this is an exciting lot of ... not too late. See below for details.) , ,So while I ... products I discovered last week while keynoting the HP Imaging and ...
... required regulatory approvals for the $2.3 billion acquisition of ... divest of two divisions of the company they are ... manufacturer plans to integrate Instrumentarium it into its ... CEO, Dow Wilson.. , ,The final required ...
... you like some fries with your Internet connection? ... began installing wireless fidelity (Wi-Fi) Internet connections in five ... These Milwaukee locations join select McDonald's restaurants in the ... Chicagoland, and Canada offering the new McDonald's Wireless Internet ...
Cached Biology Technology:Two Cool Products Advance Printing Capabilities 2Wisconsin McDonalds Go Wireless 2
(Date:3/24/2015)... , Mar. 24, 2015 Research and Markets ... and Voice Biometrics - Global Strategic Business Report" report to ... Face and Voice Biometrics in US$ Thousands by the following Segments: ... for the US, Canada , Japan ... , Middle East & Africa ...
(Date:3/23/2015)... 2015  In the 2014 fiscal year, irs.gov recorded ... This figure accounts for a fraction of the estimated ... Report, exceeds $11 billion globally. The polygraph has traditionally ... has occurred. Investor Mark Saint Juste along ... Paolella partnered to administer the test in a ...
(Date:3/20/2015)... --Research and Markets ( http://www.researchandmarkets.com/research/vkzvcs/iris_biometrics ) has announced ... Business Report" report to their offering. This ... Thousands. The report provides separate comprehensive analytics for the US, ... Europe , Asia-Pacific , ... America . Annual estimates and forecasts are ...
Breaking Biology News(10 mins):Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6Polygraph Test Now Used to Protect Investors From Fraud 2Polygraph Test Now Used to Protect Investors From Fraud 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 2Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 5
... payloads of cancer-fighting drugs to tumors in the body ... scientists are still at a relatively early stage in ... developing nanoparticles that work as "magic bullets" selectively ... still the goal, the reality is that most of ...
... from University of Utah School of Medicine researchers show that the ... who have chronic fatigue syndrome (CFS). These findings contradict a ... XMRV. The study, performed by a team of U of ... of pathology, was published May 4, 2011, in the Journal of ...
... Several members of the American Society of Plant Biologists ... of the U.S. National Academy of Sciences in recognition ... Three current ASPB members were elected to this ... Professor of Biological Sciences, University of MissouriColumbia ...
Cached Biology News:Hitting target in cancer fight now easier with new nanoparticle platform, UCLA scientists say 2Comprehensive study finds no link between XMRV retrovirus and chronic fatigue syndrome 2Comprehensive study finds no link between XMRV retrovirus and chronic fatigue syndrome 3
... The new MALDI O-TOF creates a new ... an orthogonal platform that has been licensed ... University of Manitoba. , As the first ... design and collisional cooling, the prOTOF 2000 ...
Rat Lung Fibroblasts (RLF) (>500,000 cells)...
Rat Aortic Endothelial Cells (RAOEC) (>500,000 cells)...
...
Biology Products: