Navigation Links
Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System

Software)


Method
LEADseeker setup
A flat field correction was established using a [14C] Uniform Reference Plate. No background correction was required.

Plates were imaged on the LEADseeker Multimodality Imaging System for 60 s with coincident averaging and 3 × 3 binning.

Reagent preparation
1. The kinase enzyme was diluted using the antibody reagent supplied in the kit to give a range of working stocks yielding a final concentration range in the assay from 0.05 to 100 units/well.

2. The AMARA substrate and ATP were diluted with the assay buffer supplied in the kit to yield working stocks of 20-µM AMARA substrate, 40-µM ATP, and 400-µM AMP.

3. The chemiluminescent substrate (CLS) was prepared by mixing 1 part Galacton-Star™, 5 parts Emerald-II™, and 19 parts CLS diluent.

4. The enzyme donor (ED)/CLS mix was prepared by carefully mixing 1 part ED reagent with 1 part CLS and was used within 6 h.

5. The enzyme acceptor (EA) reagent was used as supplied.

Assay procedure

1. To the kinase assay wells, add 10 µl of kinase reaction buffer to each well. To the standard curve wells, add 10 µl of AMARA standard and 10 µl of AMARA antibody reagent.

2. Incubate plate in darkness for 60 min at room temperature (20–25 ºC).

3. Add 20 µl of ED/CLS to each well.

4. Incubate plate in darkness for 60 min (± 15 min) at room temperature.

5. Add 10 µl of EA reagent to each well.

6. Incubate plate in darkness at room temperature for at least 60 min or overnight.

7.
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:7/31/2014)... CHICAGO , July 31, 2014 Nearly ... 65 th Annual Meeting & Clinical Lab Expo ... The meeting featured never-before-seen breakthroughs in diagnostic research and ... treatment they need. As of Wednesday, July ... 2014 AACC Annual Meeting & Clinical Lab Expo, with ...
(Date:7/31/2014)... people over 60 are not donor candidates for bone ... time, making the elderly prone to life-threatening infection and ... now has discovered a reason why. , "We have ... blood-forming cells to maintain blood production over time in ... could be restored for rejuvenation therapies," said Emmanuelle Passegu, ...
(Date:7/31/2014)... (1912-1954), is famous for a number of breakthroughs, which ... he published a paper, which laid the foundation of ... computer algorithm. He next played a pivotal role in ... the German military codes, enabling the Allies to defeat ... late 1940,s he turned his attention to artificial intelligence ...
(Date:7/31/2014)... even the entire body to visualize long-range connections between ... a long-time dream of biologists. A study published by ... Cell has now made that dream a ... and human tissue biopsies transparent, while keeping the cellular ... way for a better understanding of brain-body interactions, more ...
Breaking Biology Technology:Research on Digital Health, Alzheimer's, Ebola Draws Nearly 20,000 Attendees to 2014 AACC Annual Meeting & Clinical Lab Expo 2Research on Digital Health, Alzheimer's, Ebola Draws Nearly 20,000 Attendees to 2014 AACC Annual Meeting & Clinical Lab Expo 3Key to aging immune system is discovered 2A mathematical theory proposed by Alan Turing in 1952 can explain the formation of fingers 2A mathematical theory proposed by Alan Turing in 1952 can explain the formation of fingers 3See-through organs and bodies will accelerate biomedical discoveries 2
... January 12 Bavarian Nordic,owns several United States ... core technology, MVA-BN(R), which is the basis for ... a vector for delivering,recombinant vaccines. Bavarian Nordic has ... infringement action. The claim in this case is ...
... ALAMEDA, Calif., Jan. 12 Singulex, Inc. today announced ... led by JAFCO Co., Ltd. and supported by existing ... round will support the commercialization of Singulex technologies in ... Hozoji, investment officer for JAFCO Life Science Investment, will ...
... Pharmaceuticals today,announced that it has achieved its first candidate ... GlaxoSmithKline. Anacor may,receive future milestone payments and royalties ... sales. , "We are pleased ... result of,the alliance we have built between our team ...
Cached Biology Technology:Bavarian Nordic's Case Against Oxford BioMedica 2Singulex Closes $19 Million Financing Round 2Anacor Achieves Milestone from GSK for Boron-Based Systemic Antibacterial Product Candidate 2
(Date:7/31/2014)... GAINESVILLE, Fla. --- By tracing nearly 3,000 genes ... moths, University of Florida scientists have created an ... to use large-scale, next-generation DNA sequencing. , ... more closely related to small moths than to ... how butterflies evolved. The study also found that ...
(Date:7/31/2014)... Latinos are the largest minority group in the United ... of Latinos are obese and are 1.2 times as ... , NYU College of Nursing student researcher Lauren Gerchow, ... factors that contribute to this problem by compiling a ... patterns in Latina women recently published in Nursing ...
(Date:7/31/2014)... known that biomass burning burning forests to ... , slash-and-burn agriculture and wildfires figures into ... until the release of a new study by ... Z. Jacobson, the degree of that contribution had ... in a paper published July 30 in the ...
Breaking Biology News(10 mins):UF study advances 'DNA revolution,' tells butterflies' evolutionary history 2UF study advances 'DNA revolution,' tells butterflies' evolutionary history 3NYU research looks to combat US Latina immigrant obesity 2Stanford professor finds that wildfires and other burns play bigger role in climate change 2Stanford professor finds that wildfires and other burns play bigger role in climate change 3Stanford professor finds that wildfires and other burns play bigger role in climate change 4
... are important vehicles for the transmission of enteric ... including human pathogens, are particularly abundant in bivalve ... procedures, thus representing a potential risk for human ... to affect the persistence of vibrios in these ...
... population of North Pacific right whales, found during the summer ... whale populations in the world. Commercial whaling in the 1800s ... these whales have been recognized as a different species from ... the Southern Hemisphere. , To help define areas and ecological ...
... in northern China starting as far back as 2400 ... farming combined with other crops, says University of Toronto ... with Chinese agriculture, most archeologists have, until recently, thought ... food associated with northern China. However, recent archeological research ...
Cached Biology News:Edible bivalves as a source of human pathogens: signals between vibrios and the bivalve host. 2Habitat use by North Pacific right whales, Eubalaena japonica, in the Bering Sea and Gulf of Alaska 2
... The CEQ 8800 Genetic Analysis System, ... samples. This automated system adds a higher ... The CEQ 8800, part of Beckman Coulters ... bar coding link with the Biomek series liquid ...
... is a research grade spectrofluorometer which offers ... speeds of up to 60,000nm/min. Its ... concentrated light beam along with low sample ... FL Solutions, PC fluorescence applications software, which ...
... cDNA Library Panels were designed for full-length ... of PCRs to identify the desired cDNA ... in a 96-well "Master Plate," where each ... Having identified the positive well(s) by gel ...
... lication: For use as ... purification and precipitation. Physical form: ... HCl, pH 7.4, 1 mM ... ethanol precipitated Concentration: ...
Biology Products: