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Performance of fluorescent dyes with LEADseeker Multimodality Imaging System

a obtained for a variety of dyes using the standard optical configurations available with LEADseeker Multimodality Imaging System.

Performance was also assessed in fluorescence polarization mode. Here, too, we calculated the mean value of the signal and standard deviation at each concentration and then determined the LLD (where LLD was the lowest dye concentration with an observed polarization value consistent with that used as the reference value for that dye and with a standard deviation value of less than 10 mP for the replicates). Table 2 shows the data obtained for a variety of dyes using the standard optical configurations available on LEADseeker Multimodality Imaging System; data obtained using the dye-specific optical configurations is shown in Table 3.



Conclusion
• A variety of fluorescent dyes can be used in either fluorescence intensity or fluorescence polarization mode with LEADseeker Multimodality Imaging System.

• The ability to choose from standard filter sets or from dyespecific optical configurations enhances the flexibility of LEADseeker Multimodality Imaging System.



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