Navigation Links
Performance of fluorescent dyes with LEADseeker Multimodality Imaging System

Key words: fluorescence • LEADseeker • Cy • Alexa Fluor • TAMRA • Brij


Flexible and scalable uHTS programs demand that organizations be able to select the most appropriate or cost-effective fluorescent reagents for the task. This application note describes experiments to assess the performance of a variety of dyes with LEADseeker™ Multimodality Imaging System. Data was collected in the system's fluorescence intensity and fluorescence polarization modes. Lowest limits of detection (LLD) were determined for the dyes using either the standard filter sets supplied with the instrument, or a collection of filter configurations defined specifically for each dye type. Results demonstrate that a variety of dyes are fully compatible with the LEADseeker Multimodality Imaging System, rendering it a versatile detection system capable of supporting a broad range of uHTS applications.


Materials
Products used
LEADseeker Multimodality Imaging System 18-1140-71

Cy™3B; Cy5 PA63101; PA15101

Alexa FluorTM 532 optical configuration 11-0013-51
(for FP modality) consists of: Fluorescence
excitation filter 500 nm, bp 40 nm Fluorescence
perpendicular dichroic Fluorescence emission
filter 568 nm, bp 50 nm

Alexa Fluor 532 Fluorescence Epi-mirror dichroic 11-0013-73

Fluorescence Epi-mirror dichroic for 11-0013-50
BODIPYTM-TMR (in FP modality)


Other materials required

'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:5/24/2013)... May 24, 2013 Senomyx , Inc. ... taste science technologies to discover, develop, and commercialize novel ... , Ph.D., the Company,s Vice President, Biology and ... Relations & Corporate Communications, will present an overview of ... 10:25 a.m. Eastern Time (7:25 a.m. Pacific Time) during ...
(Date:5/24/2013)... 2013 Many factors impact tissues targeting ... efflux transporters at the blood-tissue barrier. Even when tissue ... fractions in the tissue rather than the total tissue ... is required to guide lead optimization, predict in vivo ... variables that may impact the likely human clinical dose. ...
(Date:5/24/2013)... Vestiage, Inc. (stock symbol "VEST") ... science-based research and development, sales and marketing of ... announced today that it is awarding exclusive Vestiage ... of Reluma Skin Illuminating Facial Care. Qualified applicants ... sending an email to info(at)vestiageinc(dot)com and putting RELUMA ...
(Date:5/23/2013)... , May 24, 2013 ... angefertigt – Bei der englischen ... , 91 % der Teilnehmer einer groß angelegten ... 20 % laut GINA [*] -Definition [1] als ... Menschen mit Asthma generell einer von vier Denkweisen zugeordnet ...
Breaking Biology Technology:SENOMYX TO WEBCAST CORPORATE PRESENTATION AT THE CITI 2013 GLOBAL CONSUMER CONFERENCE 2DMPK for Targeted Tissue Delivery: Solutions for the Most Challenging Part of Outsourcing, New Life Science Webinar Hosted by Xtalks 2Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 2Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 3Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 4Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 5Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 2Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 3Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 4Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 5Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 6Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 7
... Inc. (Nasdaq: LXRX ) today announced that Arthur ... will present,at the BIO InvestorForum at The Palace Hotel ... 11:00 a.m. Pacific Time. A live webcast of ... http://www.lexpharma.com . An archived version of the,webcast will ...
... GENZ ) announced today that it has ... the treatment of Hunter syndrome.,Elaprase is an enzyme ... and Genzyme is commercializing the product in Japan ... Shire. Genzyme intends,to launch Elaprase this quarter, following ...
... COHR ) today announced that, as anticipated, on ... letter indicating that,the Company is not currently in compliance ... 4350(e) and 4350(g) due to,its failure to hold an ... 30, 2007. The notice stated that the failure could,serve ...
Cached Biology Technology:Lexicon Pharmaceuticals to Present at Bio InvestorForum 2Genzyme Receives Approval to Market Elaprase(R) in Japan 2Genzyme Receives Approval to Market Elaprase(R) in Japan 3Genzyme Receives Approval to Market Elaprase(R) in Japan 4Coherent, Inc. Receives Expected Letter from NASDAQ 2
(Date:5/22/2013)... to improve durability in fuel cell powered buses, ... discovered links between electrode degradation processes and bus ... effects of electrode degradation stressors in the operating ... , The findings of the study, led by ... in a long-term study at Burnaby-based Ballard Power ...
(Date:5/22/2013)... genetic engineering drastically reduces the time and effort ... workhorses of biotechnology, scientists are reporting. Published in ... method paves the way for more rapid development ... and other activities. , Keith Shearwin and colleagues ... the genetic material DNA into a bacterium,s genome ...
(Date:5/22/2013)... details on how climate change will affect interactions between ... Journal of Animal Ecology . This knowledge, they ... policymakers of how species are likely to be impacted ... growing recognition among biologists that climate change is affecting ... is going to have very important consequences for the ...
Breaking Biology News(10 mins):Tests lead to doubling of fuel cell life 2UCLA life scientists present new insights on climate change and species interactions 2UCLA life scientists present new insights on climate change and species interactions 3UCLA life scientists present new insights on climate change and species interactions 4UCLA life scientists present new insights on climate change and species interactions 5
... -- (Nov. 14, 2011) -- To protect Houston and ... experts recommends building a floodgate across the Houston Ship ... on Galveston Island and the developed west side of ... coastal recreation area to sustainably use wetlands that act ...
... 14, 2011Mary Ann Liebert, Inc., publishers ( http://www.liebertpub.com ... and Technology , a highly innovative, bimonthly peer-reviewed ... has the potential to significantly improve human health, ... and innovative results, essential data, cutting-edge discoveries, thorough ...
... Hull, Bristol and Frankfurt have shown that a new ... on small samples of biological proteins, particularly proteins that ... enhanced form of nuclear magnetic resonance (NMR) spectroscopy, could ... within hours, rather than weeks or months, radically speeding ...
Cached Biology News:Protecting Houston from the next big hurricane 2Protecting Houston from the next big hurricane 3New journal on disruptive science and technology launching in 2012 2New technique enables study of 'challenging' proteins 2
E2F2 protein...
Anti-DDDDK-Tag (Enterokinase Cleavage Site), Agarose Conjugate Research Focus: tag Storage: 4C Shipping Temperature: 4C...
Goat polyclonal to XAGE1 ( Abpromise for all tested applications). Antigen: Synthetic peptide: CGFGFRRQGEDNT, corresponding to C terminal amino acids 149-160 of Human XAGE1 Entrez Gene ID: 9...
Sheep polyclonal to Staphylococcus DNase ( Abpromise for all tested applications). Antigen: Full length native protein (purified)....
Biology Products: