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Performance of fluorescent dyes with LEADseeker Multimodality Imaging System

Key words: fluorescence • LEADseeker • Cy • Alexa Fluor • TAMRA • Brij


Flexible and scalable uHTS programs demand that organizations be able to select the most appropriate or cost-effective fluorescent reagents for the task. This application note describes experiments to assess the performance of a variety of dyes with LEADseeker™ Multimodality Imaging System. Data was collected in the system's fluorescence intensity and fluorescence polarization modes. Lowest limits of detection (LLD) were determined for the dyes using either the standard filter sets supplied with the instrument, or a collection of filter configurations defined specifically for each dye type. Results demonstrate that a variety of dyes are fully compatible with the LEADseeker Multimodality Imaging System, rendering it a versatile detection system capable of supporting a broad range of uHTS applications.


Materials
Products used
LEADseeker Multimodality Imaging System 18-1140-71

Cy™3B; Cy5 PA63101; PA15101

Alexa FluorTM 532 optical configuration 11-0013-51
(for FP modality) consists of: Fluorescence
excitation filter 500 nm, bp 40 nm Fluorescence
perpendicular dichroic Fluorescence emission
filter 568 nm, bp 50 nm

Alexa Fluor 532 Fluorescence Epi-mirror dichroic 11-0013-73

Fluorescence Epi-mirror dichroic for 11-0013-50
BODIPYTM-TMR (in FP modality)


Other materials required

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