Navigation Links
Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system

Key words: adenoviral vector • viral transduction • EGFP • IN Cell Analyzer • gene delivery


Biological assays developed in cultured and primary cells can greatly aid secondary screening as well as lead and target validation, but their development presents several challenges. Establishing stable cell systems to express target genes of interest at detectable levels is time-consuming. In addition, cellular signaling is complex, with data often difficult to interpret reproducibly.

Engineered adenoviral vectors mitigate these challenges by providing facile ways to deliver key genes into target cells. The Ad-A-Gene adenoviral vector gene delivery system rapidly delivers a range of signal pathway “sensors” into mammalian target cells via viral transduction (1, 2). These sensors comprise a panel of cellular genes, each of which is fused as a chimera to either enhanced green fluorescent protein (EGFP) or emerald fluorescent protein (eFP), or a response element controlling the expression of the E. coli B nitroreductase (NTR) reporter gene (3–7). The chimeric genes become transiently expressed within transduced target cells, allowing for the development of cellular assays that exploit the signal readouts from the sensor gene markers.

This application note describes the performance of an EGFP reporter assay for assessing the translocation of mitogen-activated protein kinase-activated protein kinase2 (MAPKAP-k2). MAPK signaling pathways are instrumental in the transduction of extracellular signals into intracellular responses. For instance, one specific set of pathways mediated by p38MAPK, are activated in response to stresses such as heat shock, ultraviolet light, bacterial lipopolysaccharide (LPS) and to pro-inflammatory cytokines, such as tumor necrosis factor alpha (TNFa) and interleukin 6 (IL-6). MAPKAP-k2, a direct substrate of p38MAPK
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:12/24/2014)... PharmaBoardroom,s new report, , Healthcare and Life ... free download , digs deep into this vibrant market, speaking ... today. One area where Algeria ... homegrown pharmaceutical manufacturing base, even if the government,s ambitious target ... A cursory comparison with neighboring Morocco ...
(Date:12/24/2014)... PARIS and NEW YORK ... data from its open-label pilot study of mazindol in ... Design, Development and Therapy in December 2014 . ... of mazindol in children with attention deficit/hyperactivity disorder" ( ... 2014 Dec 1;8:2321-2332. eCollection 2014 ) ...
(Date:12/22/2014)... Dec. 22, 2014 /PRNewswire/ - RepliCel Life Sciences Inc. ... company focused on the development of autologous cell therapies, ... the University of Calgary in conjunction with co-authors from ... further validates the company,s ongoing clinical research using dermal ... pattern baldness. The paper entitled " Hair ...
(Date:12/22/2014)... VIEJO, Calif. , Dec. 22, 2014  ( ... that CoPatient, an online medical bill review and advocacy ... offering in the FREE WellCard Savings discount health services ... medical bill that was more than they expected to ... members reduce health care costs, WellCard Savings is pleased ...
Breaking Biology Technology:PharmaBoardroom Releases New Algeria Pharmaceuticals Report 2PharmaBoardroom Releases New Algeria Pharmaceuticals Report 3Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 2Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 2Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 4Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 5WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2
... Experience to the Leader in ... Cellular Bioenergetic Profiling, N. BILLERICA, ... for real-time measurement of cellular,bioenergetics in microplates, announced today that ... company,s Board of,Directors. In this position, Keegan will apply his ...
... Cadus Corporation (OTC,Bulletin Board: KDUS) announced today financial results for ... for the second quarter of 2007 and for the same ... was $218,960, compared,to net income of $75,387 for the same ... quarter of 2007 was $0.02, compared to basic net,income per ...
... a Phase II study of R1507 ... for the Treatment of Sarcoma, ... its partner, Roche has initiated a Phase II,clinical study of R1507 ... created by Genmab under the company,s,agreement with Roche and initiation of ...
Cached Biology Technology:Seahorse Bioscience Appoints Former Molecular Devices Executive Dr. Joseph D. Keegan to Board of Directors 2Cadus Reports Second Quarter 2007 Results 2Cadus Reports Second Quarter 2007 Results 3Cadus Reports Second Quarter 2007 Results 4Genmab A/S - R1507 Antibody to Enter Phase II Study to Treat Sarcoma 2
(Date:12/22/2014)... DENVER , Dec. 22, 2014  The ... for the biometrics industry as Acuity Market Intelligence ... biometrics may finally be open. Acuity forecasts that ... mobile devices that incorporate biometrics will drive a ... 4.8 billion biometric devices by 2020.   ...
(Date:12/19/2014)... --Research and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) has ... North America Perimeter Security Systems Market" report to ... The North American perimeter security market is ... 2014 to 2019. Although the U.S. market holds a ... is expected to grow at a higher CAGR ...
(Date:12/19/2014)... 18, 2014   LaunchKey , the first decentralized ... and Internet of Things era, today announced the close ... round was led by Metamorphic Ventures with participation from ... VegasTechFund, and others.  LaunchKey has raised $4 million to ... its team and bring LaunchKey to market in 2015. ...
Breaking Biology News(10 mins):First Season of Holiday Shopping with Mobile Biometric Payments Wraps Up With a Present for Biometrics Industry: A Rosy Forecast for More Than 2 Billion Users of 4.8 Billion Mobile Biometric Devices by 2020 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3
... announced today that they have entered into ... its proprietary photocleavable biotin reagent for the ... The biggest constraint on successful ... is achieving sufficient sensitivity. SomaLogic,s innovative SOMAmer ...
... 29, 2011) The future brightened for organic chemistry when ... attach organic molecules to pristine graphene, making the miracle material ... lab of chemist James Tour, building upon a set of ... method that turned what was a single-atom-thick sheet of carbon ...
... A team of scientists from Worcester Polytechnic Institute ... Life Sciences and Bioengineering Center, have regenerated functional muscle ... clinical therapy to treat people who suffer major muscle ... coax mature human muscle cells into a stem cell-like ...
Cached Biology News:Graphene lights up with new possibilities 2Graphene lights up with new possibilities 3Body rebuilding: Researchers regenerate muscle in mice 2Body rebuilding: Researchers regenerate muscle in mice 3
...
Hygromycin resistance gene (hph), provided in a pORF plasmid. pORF is an expression plasmid selectable with Ampicillin....
... template is designed to allow neomycin/kanamycin selection ... FRT-PGK-gb2-neo-FRT template encodes the neomycin/kanamycin resistance gene ... expression of kanamycin resistance in E.coli ... expression of neomycin resistance in mammalian cells. ...
... This depletion cocktail is tailored ... cells from suspensions of rat ... using the StemSep (or compatible ... selection system. The CD4+ T ...
Biology Products: