Navigation Links
Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system

e response (EC50 values) to dexamethasone in transduced cells compared favourably to that in stable cell lines, and similar Hill slope values were generated that indicate single-binding site stoichiometry in both cases.

Note: Setup of this experiment required use of different cell types and this should be considered when attempting to make direct comparisons of the resulting data.

Reproducibility of adenoviral transduction
Figure 7 shows normalized results obtained from three experiments in which transduced HeLa cells transiently expressing EGFP-GCCR were treated with varying doses of dexamethasone. The data show that adenoviral transduction is both robust and reproducible and that there is excellent agreement in EC50 values across the three experiments. Goodness-of-fit (R2) is well within acceptable limits.


Conclusion
The EGFP-GCCR chimera, when transiently expressed in host cells after adenoviral transduction, functions well in a translocation assay upon exposure to pharmacologically relevant stimuli. The response is both time and dose dependent. Use of the EGFP-GCCR adenoviral vector shows broad tropism of transduction in a range of target cells with subsequent assay results comparing favorably to those obtained in a stable cell line expressing GFP-tagged GCCR. Experimental variation, when carrying out adenoviral transduction followed by functional evaluation, is statistically acceptable.

The sequential process of viral transduction, exposure of cells to reagents, and data acquisition can be transcribed into suitable automated and integrated workflow systems with standard liquid-handling dispensing units as well as image capture and data analysis workstations.


References
1. Zhang, S. and Daniellsen, M. Steroid structures and activities [Online] http://nrr.georgetown.edu/NRR
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:10/30/2014)... October 30, 2014 2014 Deep ... Industryā€¯ is a professional and in-depth research report ... basic Naltrexone HCL information, including its definition, classification, ... industry overview. This research covers the international market ... global industry analysis covering macroeconomic environment & economic ...
(Date:10/30/2014)... 2014 Rancho BioSciences , the ... manually curated public data source TCGA for ... the data is now available for other oncology groups. ... and internal data sources for their Pharma, Government and ... the data that becomes more easily accessible. Rancho ...
(Date:10/30/2014)... York, NY (PRWEB) October 30, 2014 ... plan for its ICH Stability Testing and Package Test ... this investment centers on a dramatic increase in environmental ... volume of stability, storage and aging samples to be ... stage of this major capital expenditure has already taken ...
(Date:10/30/2014)... Oct. 30, 2014 Isis Pharmaceuticals, Inc. (NASDAQ: ... that management will present a company overview at the Nomura ... ET in Boston, MA. ... the "Investors & Media" section of the Company,s website, ... website within 48 hours and will be archived for a ...
Breaking Biology Technology:Naltrexone HCL Market & Nalbuphine HCL Industry Analysis for Global, China Regions Now at DeepResearchReports.com 2Naltrexone HCL Market & Nalbuphine HCL Industry Analysis for Global, China Regions Now at DeepResearchReports.com 3Rancho BioSciences Manually Curated TCGA Data Sets Available 2Whitehouse Labs Announces Major Capital Investment in Stability and Storage Testing Capacity 2Whitehouse Labs Announces Major Capital Investment in Stability and Storage Testing Capacity 3Isis Pharmaceuticals to Present at the Nomura Biotechnology Conference 2
... Interview with Josh Heling, co-founder, SecurePipe, Inc. ... variety of industries including manufacturing, healthcare, banking, environmental response, ... social engineering, raising money, and ecruiting a CEO. , ... a little about SecurePipe. , , Josh Heling ...
... in Entrepreneurship program is the WAVE of the future for ... Director of the Weinert School of Entrepreneurship ... behind in Wisconsin. It is a state that has a ... on here at the university, we have WARF handling, promoting ...
... companies who dont panic, that will continue to grow and ... working on a project for the Council for Continuous Improvement ... was arranging and conducting seminars for executives in the auto ... make or break situation with many quality control issues. During ...
Cached Biology Technology:WTN Exclusive Interview with Josh Heling 2WTN Exclusive Interview with Josh Heling 3WAVE of the Future? 2WAVE of the Future? 3A Race without a Finish Line! 2
(Date:10/29/2014)... all the rage. Thousands of scientists worldwide are conducting ... titanium dioxide nanoparticles from sun creams can get through ... from electronic products are as hazardous for the lungs ... food can get into the blood via the intestinal ... are flowing – and the number of scientific projects ...
(Date:10/29/2014)... An innovative workflow reveals new research potential of the ... recently published article in the Biodiversity Data ... BOLD in tabular format and imported these into a ... Writting Tool (PWT). Data were used to study ... wasps from the Microgastrinae subfamily. , BOLD is originally ...
(Date:10/29/2014)... of kidney cancer patients in Europe sheds light on ... an apparent link between exposure to aristolochic acid and ... research, by an international team led by scientists from ... Montreal, underscores the importance of investigating possible sources of ... of the Aristolochia genus, also has been suspected of ...
Breaking Biology News(10 mins):Nanosafety research: The quest for the gold standard 2Nanosafety research: The quest for the gold standard 3Go straight and publish: From Barcode of Life Data Systems to scholarly publishing systems 2Study sheds light on genetic architecture of kidney cancer 2Study sheds light on genetic architecture of kidney cancer 3
... Ph.D., member of the Basic Sciences Division at Fred ... in the American Academy of Arts & Sciences, or ... honorary societies and independent policy-research centers.She is among 190 ... the AAAS 2008 class of fellows. Drawn from the ...
... The key focus of the Diogenes study is to identify ... after initial successful weight loss. To participate in the ... least 8% of their body weight, before the whole family ... and randomised to one of five diets. 763 ...
... -- Ronald Hoenig and Aaron Welch, both graduate ... School of Marine UM Rosenstiel School students take ... Competitionand Atmospheric Science, won first place in the ... Leigh Rothschild Entrepreneurship Competition. The two Marine ...
Cached Biology News:Nobel laureate Dr. Linda Buck elected to American Academy of Arts & Sciences 2008 Class of Fellows 2Nobel laureate Dr. Linda Buck elected to American Academy of Arts & Sciences 2008 Class of Fellows 3How to stop regaining weight? That's the real question 2Aquaculture concept leaves judges 'goggle eyed' 2
Pronase Reagent...
CircuLex Anti-Human S100P Polyclonal Antibody Research Focus: proliferation Storage: -20C Shipping Temperature: 4C...
Rabbit polyclonal to ECSIT ( Abpromise for all tested applications). entrezGeneID: 51295 SwissProtID: Q96HQ7...
HAMP Immunogen: HAMP (AAH20612, 25 a.a. ~ 85 a.a) full length recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Biology Products: