Navigation Links
Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system

.g. see data for MCF7 cell line transduced for 24 h).

Agonist responses in HeLa cells transduced with the EGFP-GCCR adenoviral vector
Figure 5 shows agonist responses for a range of glucocorticoid compounds and other steroid analogues. The data show differential potency of agonists, with the expected high potency of dexamethasone (mean nucleus to cytoplasm ratio 6.33, SD 1.154) and prednisolone (mean 6.79, SD 0.938) at the GCCR. The high response to RU486 (mean 4.05, SD 0.348), which is reportedly an antagonist, appears to support the observation that RU486, in this system, is functioning as an activator of translocation, as has been noted before (2). Use of the EGFP-GCCR fusion protein enables facile identification of this phenomenon.

Function of gene sensors: Adenoviral transduction vs stable cell lines
The use of adenoviral vectors to generate the transient expression of key genes can avoid the laborious and time-consuming establishment of stable cell lines. It is therefore important to demonstrate that the functionality of the transiently expressed gene is comparable, within certain criteria, to equivalent genes expressed in stable cell lines.

Figure 6 summarizes the response to dexamethasone of target HeLa cells transiently transfected, at a range of MOI, with the EGFP-GCCR adenoviral vector. This was compared to equivalent responses in stable HEK 293 cell lines expressing GFP-GCCR. The data show MOI-dependent functional responses in target HeLa cells that have been transiently transfected with the EGFP-GCCR adenoviral vector. Maximum response, as measured by the nuclear to cytoplasm intensity ratio, was at 40 MOI. However, at all MOI values tested in this experiment, EC50 values calculated for the corresponding assays were comparable, indicating similar sensitivities to the detection of agonists across the range of MOI values employed.

Dos
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:1/22/2015)... California (PRWEB) January 22, 2015 Pipette.com ... Offer to their comprehensive portfolio of Eppendorf products. ... customers to purchase a Centrifuge 5424/5424 R and receive ... of either Eppendorf Research plus or Eppendorf Reference 2 ...
(Date:1/22/2015)... Dr. Greg Leyer of UAS Labs recently was ... Pre-Conference seminar on probiotics in San Diego, CA. , ... conference for health care professionals. This year’s pre-conference seminar was ... in health. Dr. Leyer spoke about the emerging topics and ...
(Date:1/22/2015)... January 22, 2015 Selexis SA, a ... Research Cell Banks (RCBs) used for drug discovery to ... Banks will include Next-Generation Sequencing (NGS) data ... de-risks biologic manufacturing by ensuring the integrity of the ...
(Date:1/22/2015)... Nueva Jersey , 22 de enero de 2015  El ... abre hoy su llamada a nominaciones para 2015. Este ... ha hecho, o tiene el potencial para hacer, contribuciones ... nominaciones se aceptarán hasta el 15 de marzo de ...
Breaking Biology Technology:Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4
... genetic association studies have uncovered a number of DNA ... these risk variants lie outside of genes, posing a ... cancer. In a report published online today in ... characterized a functional DNA element associated with prostate cancer, ...
... DARTMOUTH, NS , July 12 /PRNewswire/ - Ocean ... EPA/DHA, food and dietary supplement ingredients, announces today the acquisition of ... (GAT) Omega-3 microencapsulation-emulsion technology. , ... As a result of the agreement, ONC acquired the ...
... SEATTLE , July 12 Alaska Airlines ... ), Portland International Airport, Seattle-Tacoma International Airport, ... today announced a strategic initiative to promote aviation biofuel development ... kind in the United States , the "Sustainable Aviation ...
Cached Biology Technology:Prostate cancer risk variant found to be in a functional DNA sequence linked with disease 2Prostate cancer risk variant found to be in a functional DNA sequence linked with disease 3Ocean Nutrition Canada Announces Acquisition of Exclusive License to GAT Food Essentials, Omega-3 Microencapsulation-Emulsion Technology 2Ocean Nutrition Canada Announces Acquisition of Exclusive License to GAT Food Essentials, Omega-3 Microencapsulation-Emulsion Technology 3Transportation and Aviation Leaders Launch Sustainable Biofuels Initiative 2Transportation and Aviation Leaders Launch Sustainable Biofuels Initiative 3
(Date:12/17/2014)... 2014  HITLAB SM announced today its completion of ... adherence to current U.S. Food and Drug Administration (FDA) ... device and smart phone application trials utilizing the highest ... "HITLAB is determined to improve global healthcare access, quality, ...
(Date:12/17/2014)... Dec. 16, 2014 Research and Markets ( ... "Global Chemical Sensor Market 2015-2019" report to their ... One major trend upcoming in this market is ... Chemical sensors help in recording of patient data for ...
(Date:12/15/2014)... , Dec. 12, 2014 Research and Markets ... the "Global Facial Recognition Market 2015-2019" report ... Facial recognition is a technology used ... the overall facial feature of a person such as ...
Breaking Biology News(10 mins):HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3
... late spring, the 4000 elk of the Clarks Fork ... the greening grass into the highlands of the Absaroka ... vegetation fed by snowmelt. It,s a short trip (40-60 ... free of roads, fences, metropolitan areas, and other human-built ...
... the first time, researchers investigated enteral nutrition and caloric ... report published in the Journal of the Academy ... showed the value of including registered dietitians in the ... intestine, or enteral route, to critically ill adults has ...
... Higher levels of omega-3 fatty acids in the blood may ... research suggests. Scientists analyzed red blood cell samples from ... hip. The study showed that higher levels of omega-3 fatty ... cells were associated with a lower likelihood of having fractured ...
Cached Biology News:Declining fortunes of Yellowstone's migratory elk 2Declining fortunes of Yellowstone's migratory elk 3Declining fortunes of Yellowstone's migratory elk 4Registered dietitians help critically ill children get necessary nutrition for recovery 2Could a diet high in fish and flax help prevent broken hips? 2Could a diet high in fish and flax help prevent broken hips? 3
Trop-2 Purified Anti-Human clone 162-46, Isotype Mouse IgG 1 , 100 µg Consult technical datasheet for details....
... Enhancer System is used in ... optimize PCR of problematic and/or ... Enhancer Solution with 10X PCRX ... specificity broader magnesium concentration broader ...
... Clone/PAD: ZMD.473. Immunogen: Synthetic peptide ... the mouse junctophilin-2. Specificity: Specific ... Mouse (positive controls: mouse skeletal muscle ... Applications: Western blotting Immunohistochemistry(froz) ImmunofluorescenceStorage: ...
Abin-2 (N-16)...
Biology Products: