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Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system

as monitored by analyzing target cells transduced with the adenoviral vector, EGFP-GCCR. Monitoring the cellular position and signal intensity of the transgene product allowed examination of the distribution of GCCR within the nucleus and cytoplasm. Cellular distribution of EGFP-GCCR protein was shown to alter in response to drugs, such as dexamethasone and prednisolone.

Quantitation of the EGFP signal from the EGFP-GCCR fusion protein within the cells when exposed to chemical stimuli, gives an indication of the potency of those stimuli. Signal monitoring and quantitation was performed on the IN Cell Analyzer 1000 and IN Cell Analyzer 3000 imaging systems.


Materials
Reagents and instrumentation
Ad-A-Gene EGFP-Glucocorticoid Receptor GDS 20008

IN Cell Analyzer 1000 25-8010-26

IN Cell Analyzer 1000 Seat License* 25-8098-22

IN Cell Analyzer 3000 25-8010-11

IN Cell Analyzer 3000 Seat License* 63-0055-97

Object Intensity Analysis Module 25-8010-56
(for IN Cell Analyzer 1000)

Object Intensity Analysis Module 63-0048-93
(for IN Cell Analyzer 3000)

Nuclear Trafficking Analysis Module 25-8010-31
(for IN Cell Analyzer 1000)

Nuclear Trafficking Analysis Module 63-0048-94
(for IN Cell Analyzer 3000)

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