Navigation Links
Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system

viral gene delivery are relatively simple compared to plasmid-based transfection techniques and data can be obtained more rapidly. Transfection efficiency is efficent and uniform, with each target cell carrying at least one copy of the CRE-NTR gene under the conditions employed. In addition, this work suggests that the sequential process of viral transduction, exposure of cells to reagents, and data acquisition can be transcribed into suitable automated and integrated workflow systems with standard liquid-handling dispensing units as well as image capture and data analysis workstations.


References
1. Graham F.L., et al,. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36, 59–74 (1977).

2. Krougliak, V. and Graham, F.L., Development of cell lines capable of complementing E1, E4, and protein IX defective adenovirus type 5 mutants. Hum. Gene Ther. 6, 1575–86 (1995).

3. Kozarsky, K.F. and Wilson, J.M., Gene therapy: adenovirus vectors. Curr. Opin. Genet.. Dev. 3, 499–503 (1993).

4. Lochmuller, H., et al., Emergence of early region 1-containing replication-competent adenovirus in stocks of replication-defective adenovirus recombinants (delta E1 + delta E3) during multiple passages in 293 cells. Hum. Gene Ther. 5, 1485–91 (1994).

5. Ng, P. et al., An enhanced system for construction of adenoviral vectors by the two plasmid rescue method. Hum. Gene Ther. 11, 693–699 (2000).

6. Zhu, J. et al., Characterization of replication-competent adenovirus isolates from large-scale production of a recombinant adenoviral vector. Hum. Gene Ther. 10, 113–21 (1999).

7. Russell, W.C.,Update on adenovirus and its vectors. J. Gen. Virol. 81 (11), 2573–604 (2000).

'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:8/29/2014)... 2014 Pittcon is pleased to announce ... Koichiro Matsuda, Horiba Scientific, have organized a session on ... JASIS , Asia’s largest analytical and scientific instruments ... Messe, Japan. , The four speakers in this ... Single Molecules to Single Cells” will present their use ...
(Date:8/29/2014)... 29, 2014 A major challenge before ... start-up dense realm of Boston-Cambridge, is gaining visibility that ... James Sherley, the Director of Boston’s Adult Stem Cell ... and national visibility of his company an important priority ... In addition to a social media marketing campaign ...
(Date:8/28/2014)... Lawrence, MA (PRWEB) August 28, 2014 “This ... single test to screen from 0 to 150 ppb,” said ... test is a significant benefit to plant owners and USDA-GIPSA ... corn and other commodities. Testing can take place in a ... Not only can the plant test the feed and grain ...
(Date:8/28/2014)... BOSTON , Aug. 28, 2014  Next month, executives ... at three key industry events beginning with Patient-Centered Clinical ... Hotel in Boston , September 4-5. Patient ... , and Aaron Fleishman will share insights on ... how a variety of tactics – from media to mobile ...
Breaking Biology Technology:Fifth Annual U.S. Symposium at JASIS Organized by Pittcon’s Program Chairman Hub MacDonald 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 3The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 4BBK Worldwide Leads Sessions at Key September Events 2
... Funding Milestone Continues Progress at Seneca, Illinois ... ... NBF ) announced today that it has satisfied the conditions ... million,senior secured credit facility with WestLB AG, one of the world,s ...
... Phase 2b Trial Expected to Begin in the ... 4 Mpex Pharmaceuticals, Inc. today,announced that the ... Products Development has granted Mpex orphan drug designation ... pulmonary,infections due to Pseudomonas aeruginosa and other bacteria ...
... the Chinese Pharmaceutical ... CHENGDU, China, March 4 /Xinhua-PRNewswire-FirstCall/ -- Tianyin,Pharmaceutical, ... and,supplier of modernized traditional Chinese medicine ("TCM") based ... members:,Professor Zunjian Zhang, Ph.D., Professor Jianping Hou, Ph.D. ...
Cached Biology Technology:Nova Biosource Fuels Announces First Funding Under $41 Million Senior Secured Credit Facility 2Mpex Candidate, MP-376, Granted U.S. Orphan Drug Status for the Treatment of Cystic Fibrosis 2Mpex Candidate, MP-376, Granted U.S. Orphan Drug Status for the Treatment of Cystic Fibrosis 3Tianyin Pharmaceutical Appoints Three New Independent Board Members 2Tianyin Pharmaceutical Appoints Three New Independent Board Members 3Tianyin Pharmaceutical Appoints Three New Independent Board Members 4
(Date:8/28/2014)... A free mobile app called TX Invasives is now ... The University of Texas at Austin for identifying harmful ... , Invasive species in the United States cause about ... they destroy agricultural crops, clog waterways, kill native plants ... TX Invasives app for reporting where invasive species occur, ...
(Date:8/28/2014)... German . ... to a striking pattern of blue stripes alternating with golden ... cells, and yellow cells emerge during growth in the skin ... mosaic to compose the characteristic colour pattern. ,While ... interact to form proper stripes, the embryonic origin of the ...
(Date:8/28/2014)... 2014) Researchers at the University of Minnesota have ... (FSHD) to be used for muscle regeneration research as ... for FSHD. , The research is published in the ... , There is no treatment for FSHD, which is ... of muscular dystrophy. FSHD is an unusual genetic disorder ...
Breaking Biology News(10 mins):Mobile app makes ID of harmful plants, insects in Texas a snap 2How the zebrafish gets its stripes 2How the zebrafish gets its stripes 3UMN researchers find animal model for understudied type of muscular dystrophy 2
... The tummy,s taste for red wine with red meat , ... happens when red wine meets red meat? If the rendezvous ... bounty of healthful chemical compounds may thwart formation of harmful ... study, which reinforces the benefits of consuming wine and other ...
... closer to understanding the recent demise of billions of honey ... a common bee virus. Deformed wing virus (DWV) is passed ... parasitic mite called Varroa destructor when it feeds. However, research ... General Virology suggests that the virus does not replicate ...
... of Crohn,s disease researchers has combined data from ... variants associated with the inflammatory bowel disorder, bringing ... Several of these are involved with the ... evidence that disruptions in a process called autophagy ...
Cached Biology News:American Chemical Society's Weekly PressPac -- June 25, 2008 2American Chemical Society's Weekly PressPac -- June 25, 2008 3American Chemical Society's Weekly PressPac -- June 25, 2008 4American Chemical Society's Weekly PressPac -- June 25, 2008 5American Chemical Society's Weekly PressPac -- June 25, 2008 6American Chemical Society's Weekly PressPac -- June 25, 2008 7American Chemical Society's Weekly PressPac -- June 25, 2008 8American Chemical Society's Weekly PressPac -- June 25, 2008 9Bee disease a mystery 2International team identifies 21 new genetic risk factors for Crohn's disease 2
...
Red Standard is sodium salt of resorufin....
X-Gal, 100 mg. 5-Bromo-4-chloro-3-indolyl-b-d-galactoside.Histochemical substrate for b-galactosidase, used in blue/white screening.Store at -20 C. Protect from moisture. Category: Nucleotides & Enzy...
Oligodendrocyte Marker O1 MAb (Clone O1)...
Biology Products: