( viral gene delivery are relatively simp...)Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system

viral gene delivery are relatively simple compared to plasmid-based transfection techniques and data can be obtained more rapidly. Transfection efficiency is efficent and uniform, with each target cell carrying at least one copy of the CRE-NTR gene under the conditions employed. In addition, this work suggests that the sequential process of viral transduction, exposure of cells to reagents, and data acquisition can be transcribed into suitable automated and integrated workflow systems with standard liquid-handling dispensing units as well as image capture and data analysis workstations.

References
1. Graham F.L., et al,. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36, 59–74 (1977).

2. Krougliak, V. and Graham, F.L., Development of cell lines capable of complementing E1, E4, and protein IX defective adenovirus type 5 mutants. Hum. Gene Ther. 6, 1575–86 (1995).

3. Kozarsky, K.F. and Wilson, J.M., Gene therapy: adenovirus vectors. Curr. Opin. Genet.. Dev. 3, 499–503 (1993).

4. Lochmuller, H., et al., Emergence of early region 1-containing replication-competent adenovirus in stocks of replication-defective adenovirus recombinants (delta E1 + delta E3) during multiple passages in 293 cells. Hum. Gene Ther. 5, 1485–91 (1994).

5. Ng, P. et al., An enhanced system for construction of adenoviral vectors by the two plasmid rescue method. Hum. Gene Ther. 11, 693–699 (2000).

6. Zhu, J. et al., Characterization of replication-competent adenovirus isolates from large-scale production of a recombinant adenoviral vector. Hum. Gene Ther. 10, 113–21 (1999).

7. Russell, W.C.,Update on adenovirus and its vectors. J. Gen. Virol. 81 (11), 2573–604 (2000).

'"/>

Source:

Page: All 1 2 3 4 5 6 7 8
Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis