Navigation Links
Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system

tes were incubated at 37 ºC for 5 h, after which 10-μM CytoCy5S substrate in assay medium (10 μl) was added to give a final concentration of 1 μM. The solution was incubated for an additional 3 h at 37 ºC.

Solutions were decanted from the wells and fixing solution was added (100 μl/well), followed by 15-min incubation at room temperature. Fixing solution was decanted from all wells, and the cells were washed with PBS (200 μl/well). Finally, Hoechst nuclear dye (2.5 μM) in PBS was added (100 μl/well), and the wells were incubated for 20 min at room temperature (the plates can be stored at 2–8 °C at this stage if imaging is not performed immediately).

Plates were imaged on IN Cell Analyzer 1000 using 620/60-nm excitation and 700/75-nm emission filters (CytoCy5S). Images were analyzed using the Object Intensity Analysis Module. Plates were read on a Tecan Ultra using 610/20-nm excitation and 680/30-nm emission filters (CytoCy5S).

†Time-controlled dispensing of compound solutions to the cells can be avoided by completing the assay using live cells and then fixing the cells prior to imaging. Cells should be fixed at the peak induction time point. Fixing of cells reduces the handling requirements for post-assay material to BSL-1 categorization.


Assay protocol (kinetic live-cell)
Test compound (micromolar) was prepared from stocks in assay buffer, medium was removed from the cells, and the cells were washed with PBS. Test wells were treated with 90 μl of forskolin in assay medium; 90 μl of medium alone was added to control wells. Plates were incubated at 37 ºC for 5 h, after which 10-μM CytoCy5S substrate in assay medium (10 μl) was added to give a final concentration of 1 μM. The solution was incubated for an additional 3 h at 37 ºC followed by reading on a Tecan Ultra.

Note: At all stages of the live-cell ass
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:8/29/2014)... August 29, 2014 The global ... billion in 2019. It is expected to grow ... 2019, and was valued at $1.8 billion in ... Transparency Market Research. , For more information regarding ... . , The research report, titled “Companion Diagnostics ...
(Date:8/29/2014)... Pittcon is pleased to announce that the ... Horiba Scientific, have organized a session on nanotechnology for ... , Asia’s largest analytical and scientific instruments show, will ... , The four speakers in this U.S. symposium, ... to Single Cells” will present their use of nanotechnology ...
(Date:8/29/2014)... 2014 Local veterinarian, Dr. Keith Clement, ... seeking candidates to participate in an investigational study of ... ultimate goal of this study is to determine if ... or two arthritically affected joints can help reduce pain ... for the current investigational study must be older than ...
(Date:8/29/2014)... Due to a misstatement posted by the Family ... Technology Center, LLC ( ASCTC ) was reported as an ... might benefit ALS patients. Because of ASCTC's exclusive focus ... and therapeutic applications, FRC listed ASCTC as one of several ... wish not to support research that requires the death of ...
Breaking Biology Technology:Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 2Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 3Fifth Annual U.S. Symposium at JASIS Organized by Pittcon’s Program Chairman Hub MacDonald 2Burnt Hills Veterinary Hospital Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2The Adult Stem Cell Technology Center, LLC Declines ALS Ice Bucket Challenge Donors Wishing to Support Ethical Research for New Stem Cell Therapies 2The Adult Stem Cell Technology Center, LLC Declines ALS Ice Bucket Challenge Donors Wishing to Support Ethical Research for New Stem Cell Therapies 3
... 8 Edwards Lifesciences,Corporation (NYSE: EW ), the ... the company will celebrate a number of cardiac surgery,milestones ... the American,Association for Thoracic Surgery (AATS), May 10-14 in ... -- which this year celebrates,its 50th anniversary of partnering ...
... Listing and Attract Additional Investors ... as Company Enters Next Growth Phase, SAN DIEGO, May 8 ... (ICG),technology, today announced the reverse split of 1 for 7 shares ... Company,s,Annual Shareholders Meeting earlier today., The record date for the ...
... ... U.S. ThermaCool NXT System Sales ... THRM ), a leader in non-invasive tissue tightening in the ... 31, 2008., Revenue for the first quarter of 2008 was ...
Cached Biology Technology:Edwards Lifesciences Celebrates One Million Heart Valve Patients and More at AATS 2008 2Edwards Lifesciences Celebrates One Million Heart Valve Patients and More at AATS 2008 3Edwards Lifesciences Celebrates One Million Heart Valve Patients and More at AATS 2008 4CardioDynamics Shareholders Approve One-for-Seven Reverse Split of Company's Common Stock and Trading in Post-split Shares Begins May 9, 2008 2CardioDynamics Shareholders Approve One-for-Seven Reverse Split of Company's Common Stock and Trading in Post-split Shares Begins May 9, 2008 3Thermage Announces First Quarter 2008 Financial Results 2Thermage Announces First Quarter 2008 Financial Results 3Thermage Announces First Quarter 2008 Financial Results 4Thermage Announces First Quarter 2008 Financial Results 5Thermage Announces First Quarter 2008 Financial Results 6Thermage Announces First Quarter 2008 Financial Results 7Thermage Announces First Quarter 2008 Financial Results 8Thermage Announces First Quarter 2008 Financial Results 9
(Date:8/28/2014)... The Entomological Society of America (ESA) is pleased to ... Honorary Member of the Society. Honorary Membership acknowledges those ... through significant involvement in the affairs of the Society ... honor are selected by the ESA Governing Board and ... will be honored at the Awards Ceremony at Entomology ...
(Date:8/28/2014)... neighbors than traditional cigarettes, but still release toxins ... from USC. , Scientists studying secondhand smoke from ... to harmful particles, with close-to-zero exposure to organic ... metals in second-hand e-cigarette smoke were found to ... high levels of polycyclic aromatic hydrocarbons cancer-causing ...
(Date:8/28/2014)... Access to Research Careers) Program has announced the ... America,s 27th Annual Mouse Molecular Genetics Conference from ... Grove, California. These awards are meant to ... scientists from underrepresented groups into the mainstream of ... participation of young scientists at the Genetics Society ...
Breaking Biology News(10 mins):Fred Baxendale selected as Honorary Member of the Entomological Society of America 2Second-hand e-cig smoke compared to regular cigarette smoke 2
... John Wiley & Sons, Inc., has announced a new partnership ... Access journal Ecology and Evolution . This latest partnership ... access title to 16. Eleven of these partner titles are ... for Scientific Information (ISI). BES journals will join other ...
... Warmer oceans in the future could significantly alter populations ... on climate change. In the current issue of ... the end of the 21st century, warmer oceans will cause ... and may shrink in equatorial waters. Since phytoplankton play a ...
... A new University of Michigan study shows that when ... likelihood of forming new collaborations and obtaining funding increases ... well., "Our analyses clearly show that there are benefits ... sociology and organizational studies. Researchers who occupy the ...
Cached Biology News:British Ecological Society partners with Wiley open access journal Ecology and Evolution 2Small organisms could dramatically impact world's climate 2Sharing space: 2Sharing space: 3
... Labsystems Wellmix is a high-quality four-place microplate ... The shaking speed is adjustable from 100 ... speed and a small orbit ensure effective ... time is quick and easy to set ...
Mouse monoclonal [AZ24] to Polo like kinase ( Abpromise for all tested applications)....
... The dotLab System brings together ... diffraction and immobilized capture surfaces. This ... simple technique for the detection of ... of fluorescent labels. , Protein-specific capture ...
... LabWorks Software provides a comprehensive experiment ... use drop down menus yet sophisticated and ... with dozens of CCD cameras for easy ... , Automated Digital Video Playback ...
Biology Products: