Navigation Links
Performance Comparisons of Commercial RT-PCR Systems


prostar RT-PCR systems excel at amplifying challenging targets

Michael Borns Janice Cline Holly Hogrefe
Stratagene

We compared Stratagenes prostar RT-PCR systems to other commercially available RNA amplification kits to see how well they performed. The ProSTAR HF single-tube RT-PCR system, featuring the TaqPlus Precision DNA polymerase blend * and a convenient one-step, one-tube format, provides greater target-length capability and higher product yields than other one-tube RT-PCR kits. The ProSTAR ultra HF RT-PCR system, featuring PfuTurbo DNA polymerase** and a versatile two-step format, amplifies the longest targets and produces the lowest mutation frequencies of any RNA amplification system tested. The superior accuracy of the ProSTAR ultra HF RT-PCR system is directly related to the use of PfuTurbo DNA polymerase for cDNA amplification.

Stratagene recently introduced the ProSTAR line of RNA amplification reagents, featuring the ProSTAR HF (high fidelity) single-tube and the ProSTAR ultra HF (ultra-high fidelity) RT-PCR systems.1 The ProSTAR HF single-tube RT-PCR system combines the sensitivity and accuracy of the TaqPlus Precision DNA polymerase blend2 with the convenience of a one-step, one-tube procedure. With the ProSTAR ultra HF RT-PCR system, a versatile two-step format is used, which features PfuTurbo DNA polymerase,3 a special formulation of Pfu DNA polymerase and a novel PCR-enhancing factor*** for amplifying cDNAs with the highest replication fidelity possible. In a previous Strategies article, it was demonstrated that the ProSTAR RT-PCR systems are useful in applications requiring robust, sensitive, high-fidelity amplification of RNA from a variety of RNA sources.1

Here, we compare the performance of Stratagenes ProSTAR RT-
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
6. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
7. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
8. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
9. Highly Sensitive microRNA Array Performance
10. High Throughput RNA Isolation for High Performance Microarray Analysis
11. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Performance Comparisons Commercial PCR Systems

(Date:8/27/2015)... The National Necrotizing Fasciitis Foundation ... by doctors at Vanderbilt University Hospital in ... and Dr. John Crew , director of the ... Center in Daly City, CA. ... pioneered by Dr. Crew, that had previously saved the ...
(Date:8/27/2015)... ... August 27, 2015 , ... Inc. Magazine released its annual list of the ... 1,361 in growth for the three years through 2014. Being named to the Inc. ... organizations in the country. , “We are thrilled to make the Inc. 5000 ...
(Date:8/26/2015)... - iCo Therapeutics ("iCo" or "the Company") (TSX-V: ICO) ... quarter ended June 30, 2015. Amounts, unless specified ... under International Financial Reporting Standards ("IFRS"). ... Oral Amp B program, rapidly advancing towards the ... Rae , President & CEO of iCo Therapeutics. "We ...
(Date:8/26/2015)... 2015 Intrexon Corporation (NYSE: XON ), ... of its previously announced public offering of common stock, ... their option to purchase an additional 731,707 shares of ... per share.  The exercise of the underwriters, option brought ... by Intrexon to 5,609,756 shares and increased the total ...
Breaking Biology Technology:Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3iLab Solutions Named to 2015 Inc. 5000 List 2iLab Solutions Named to 2015 Inc. 5000 List 3iCo Therapeutics Announces Second Quarter 2015 Financial Results 2iCo Therapeutics Announces Second Quarter 2015 Financial Results 3Intrexon Announces Closing of $230 Million Public Offering of Common Stock including Full Exercise of Underwriters' Option to Purchase Additional Shares 2Intrexon Announces Closing of $230 Million Public Offering of Common Stock including Full Exercise of Underwriters' Option to Purchase Additional Shares 3Intrexon Announces Closing of $230 Million Public Offering of Common Stock including Full Exercise of Underwriters' Option to Purchase Additional Shares 4
... Highlights: -- $1.2 million contract signed for Phase III schizophrenia study, ... ... million -- $3 ... CogState Ltd (ASX: CGS),today announced that it has signed a contract with an ...
... and GUANGZHOU, China, August 12, - Major ... in U.S. Animal Models, An internationally renowned ... from experiments conducted by his,research team. This study ... in the treatment and prevention of,H5N1, H1N1 and ...
... BioSpecifics,Technologies Corp. (OTC Bulletin Board: BSTC.OB), a biopharmaceutical,company ... results for the second quarter ended June 30, 2008., ... for the quarter ended June,30, 2008, or 14 cents ... of,$478,000, or 9 cents per diluted common share, for ...
Cached Biology Technology:Sales Contracts Worth $2.3 Million Signed Since 1 July 2008 2Canopus Biopharma and Leading Chinese Researcher Team Up to Treat Avian Influenza, to Prevent Possible Pandemic 2Canopus Biopharma and Leading Chinese Researcher Team Up to Treat Avian Influenza, to Prevent Possible Pandemic 3Canopus Biopharma and Leading Chinese Researcher Team Up to Treat Avian Influenza, to Prevent Possible Pandemic 4BioSpecifics Technologies Corp. Reports Second Quarter 2008 Financial Results 2BioSpecifics Technologies Corp. Reports Second Quarter 2008 Financial Results 3BioSpecifics Technologies Corp. Reports Second Quarter 2008 Financial Results 4BioSpecifics Technologies Corp. Reports Second Quarter 2008 Financial Results 5BioSpecifics Technologies Corp. Reports Second Quarter 2008 Financial Results 6
(Date:8/10/2015)... The latest 364 page report from business intelligence provider ... border security market . Visiongain assesses that this ... Now: Border security is hard to achieve and harder to ... you need to know about - and more importantly, you ... this will impact your company and the border security industry ...
(Date:8/5/2015)... , August 5, 2015 Transparency ... "Facial Recognition Market - Global Industry Analysis, Size, Share, ... market for facial recognition is forecast to reach US$ ... increased demand for surveillance systems by civil and government ... of crimes and terrorist activities across the globe that ...
(Date:7/31/2015)... Den 10. internationale konference om Genomik (ICG-10, ... - 25. oktober i Shenzhen , ... i år. Siden starten i 2006 er ICB blevet ... og er en af de mest dynamiske, entusiastiske og ... nylige gennembrud og fremskridt inden for ,omik,-forskning, som yderligere ...
Breaking Biology News(10 mins):Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 2Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 4Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 5Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 6Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 7Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 8Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 9Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 2Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 3Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 4Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 5Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 6Den 10. internationale konference om Genomics (ICG-10) åbner i oktober 2
... feeds on a native tree from the western Pacific island of ... are out of reach for its only local natural enemy," said ... ecologist Thomas Marler to study the relationship between the native cycad ... alien insect pest called cycad aulacaspis scale (CAS). The pest arrived ...
... comparative analysis to date of bacterial communities inhabiting the ... into why some individuals become colonized with pathogens while ... in mBio the online open-access journal published by the ... are important sites of pathogen colonization, yet the microbiota ...
... that increasing certain proteins in the blood vessels of ... The study provides new avenues for research that may ... demonstrates that cytochrome P450 plays an important role in ... enormous public health concern," said Darryl Zeldin, M.D., acting ...
Cached Biology News:Cycad pest uses small size to hide from predators 2Study examines, compares bacteria in the nose and throat 2NIH-supported study finds novel pathway may open doors for new blood pressure treatments 2
... be upgraded to the GenePix Autoloader 4200AL. ... and saves results for up to 36 ... the batch is complete. Line-by-line autofocus ensures ... GenePix Pro gives you complete flexibility to ...
... Maximize the value of your microarray ... the support you need from consultation to ... to date methods. Utilize our expertise in ... or from your own microarray experiments., ,Normalization ...
... the value of your microarray data ... the support you need from consultation ... most up to date methods. Utilize ... Gene Expression Profiling Service or from ...
... CLS number is a new ... match Cornings product number. If ... order under the old Sigma-Aldrich ... service for assistance. Material: ...
Biology Products: