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Performance Comparisons of Commercial RT-PCR Systems


prostar RT-PCR systems excel at amplifying challenging targets

Michael Borns Janice Cline Holly Hogrefe
Stratagene

We compared Stratagenes prostar RT-PCR systems to other commercially available RNA amplification kits to see how well they performed. The ProSTAR HF single-tube RT-PCR system, featuring the TaqPlus Precision DNA polymerase blend * and a convenient one-step, one-tube format, provides greater target-length capability and higher product yields than other one-tube RT-PCR kits. The ProSTAR ultra HF RT-PCR system, featuring PfuTurbo DNA polymerase** and a versatile two-step format, amplifies the longest targets and produces the lowest mutation frequencies of any RNA amplification system tested. The superior accuracy of the ProSTAR ultra HF RT-PCR system is directly related to the use of PfuTurbo DNA polymerase for cDNA amplification.

Stratagene recently introduced the ProSTAR line of RNA amplification reagents, featuring the ProSTAR HF (high fidelity) single-tube and the ProSTAR ultra HF (ultra-high fidelity) RT-PCR systems.1 The ProSTAR HF single-tube RT-PCR system combines the sensitivity and accuracy of the TaqPlus Precision DNA polymerase blend2 with the convenience of a one-step, one-tube procedure. With the ProSTAR ultra HF RT-PCR system, a versatile two-step format is used, which features PfuTurbo DNA polymerase,3 a special formulation of Pfu DNA polymerase and a novel PCR-enhancing factor*** for amplifying cDNAs with the highest replication fidelity possible. In a previous Strategies article, it was demonstrated that the ProSTAR RT-PCR systems are useful in applications requiring robust, sensitive, high-fidelity amplification of RNA from a variety of RNA sources.1

Here, we compare the performance of Stratagenes ProSTAR RT-
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