Navigation Links
Performance Comparisons of Commercial RT-PCR Systems


prostar RT-PCR systems excel at amplifying challenging targets

Michael Borns Janice Cline Holly Hogrefe
Stratagene

We compared Stratagenes prostar RT-PCR systems to other commercially available RNA amplification kits to see how well they performed. The ProSTAR HF single-tube RT-PCR system, featuring the TaqPlus Precision DNA polymerase blend * and a convenient one-step, one-tube format, provides greater target-length capability and higher product yields than other one-tube RT-PCR kits. The ProSTAR ultra HF RT-PCR system, featuring PfuTurbo DNA polymerase** and a versatile two-step format, amplifies the longest targets and produces the lowest mutation frequencies of any RNA amplification system tested. The superior accuracy of the ProSTAR ultra HF RT-PCR system is directly related to the use of PfuTurbo DNA polymerase for cDNA amplification.

Stratagene recently introduced the ProSTAR line of RNA amplification reagents, featuring the ProSTAR HF (high fidelity) single-tube and the ProSTAR ultra HF (ultra-high fidelity) RT-PCR systems.1 The ProSTAR HF single-tube RT-PCR system combines the sensitivity and accuracy of the TaqPlus Precision DNA polymerase blend2 with the convenience of a one-step, one-tube procedure. With the ProSTAR ultra HF RT-PCR system, a versatile two-step format is used, which features PfuTurbo DNA polymerase,3 a special formulation of Pfu DNA polymerase and a novel PCR-enhancing factor*** for amplifying cDNAs with the highest replication fidelity possible. In a previous Strategies article, it was demonstrated that the ProSTAR RT-PCR systems are useful in applications requiring robust, sensitive, high-fidelity amplification of RNA from a variety of RNA sources.1

Here, we compare the performance of Stratagenes ProSTAR RT-
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
6. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
7. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
8. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
9. Highly Sensitive microRNA Array Performance
10. High Throughput RNA Isolation for High Performance Microarray Analysis
11. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Performance Comparisons Commercial PCR Systems

(Date:9/19/2014)... to move data would consume much less power ... as chips, transistor counts rise. , Of the ... emitters, modulators, and detectors emitters are the ... optical chips is molybdenum disulfide (MoS2), which has ... atom-thick layer. Other experimental on-chip light emitters have ...
(Date:9/19/2014)... SAN FRANCISCO , Sept. 19, 2014 /PRNewswire/ ... announced results from preclinical studies characterizing the analgesic ... oral, peripherally-acting kappa opioid receptor agonist molecules. The ... molecule polymer medicinal chemistry platform. The ... described in the medical literature. 1,2 Kappa ...
(Date:9/19/2014)... 2014 Cereal grain scientists, quality ... gather October 5–8, 2014, for the AACC International ... Center in Providence, Rhode Island. The comprehensive scientific ... food scientists, chemists, microbiologists, nutritionists, and those interested ... management. To date, 1,000 attendees have registered for ...
(Date:9/18/2014)... USA (PRWEB) September 18, 2014 Bipartisan ... of a bill designed to stimulate development and commercialization ... is being praised by leaders of SPIE, the ... the Revitalizing American Manufacturing and Innovation (RAMI) Act, now ... bill’s authors have said they are optimistic that it ...
Breaking Biology Technology:Toward optical chips 2Toward optical chips 3Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 2Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 3Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 4Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 5Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 2Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 3House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 2House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 3
... ... EDMONTON, May 9 /PRNewswire-FirstCall/ - BioMS Medical Corp (TSX: MS),a ... update on its pipeline products, HYC750 and development with,BioCyDex Inc., ... Bio. Inc. for HYC750, a technology based on hyaluronic acid that,has ...
... May 9 The Alexander von Humboldt,Foundation, one of ... has named Dr. Robert Knight as a winner of ... area of neurobiology.,It is the only prize that the ... Germany, the Humboldt Foundation reviews a,select group of international ...
... 9 ATS Medical, Inc. (Nasdaq:,ATSI), manufacturer and ... it will participate at the Rodman & Renshaw,5th ... Beach Plaza,Hotel in Monte Carlo on Tuesday, May ... Medical, will present at 9:20 a.m. local time., ...
Cached Biology Technology:BioMS Medical Provides Update On Pipeline Products 2BioMS Medical Provides Update On Pipeline Products 3NeuroFocus Chief Science Advisor Wins Humboldt Prize 2ATS Medical to Participate at the Rodman & Renshaw 5th Annual Global Healthcare Conference 2
(Date:9/18/2014)... ANTONIO, September 18, 2014 New research into the ... causes a severe hemorrhagic disease in humans similar to ... essential for CCHFV infection. This discovery has the potential ... the pathogen. , The research, reported in ... Pathogens and conducted by scientists at the Texas ...
(Date:9/18/2014)... prompts millions of Americans to undertake jaunts into the countryside ... little longer within a century, according to new research. , ... of the United States as summer temperatures linger later into ... Global Ecology and Biogeography . For instance, the paper birch ... of New Hampshire could change color one to three ...
(Date:9/18/2014)... across racial and ethnic groups describe losing eyesight ... day-to-day life, more so than other conditions including: ... of African-Americans, 49% of non-Hispanic whites, 43% of ... disease or ailment is the worst that could ... followed by AIDS/HIV. Hispanics and Asians ranked ...
Breaking Biology News(10 mins):Research milestone in CCHF virus could help identify new treatments 2Fall foliage season may be later, but longer on warmer Earth 2Fall foliage season may be later, but longer on warmer Earth 3Fall foliage season may be later, but longer on warmer Earth 4Americans rate losing eyesight as having greatest impact on their lives 2Americans rate losing eyesight as having greatest impact on their lives 3
... has won a $1.175 million grant that will support its ... Geurts, who has spent his career looking for clues to ... contents of gold, lead and other heavy atoms, said the ... constructing and commissioning a highly complex detector system to using ...
... that sheds new light on the causes of Parkinson,s disease, ... energy-producing machinery, the mitochondria. A shutdown in fuel can have ... of the body,s energy despite making up only 2 percent ... the mitochondria with FDA approved drugs early on may prevent ...
... billion last year in health research from all public ... That amount represents only 5.6% of the $2.47 trillion ... health dollarwhich varies no more than 0.2% from 2005 ... http://www.researchamerica.org/uploads/healthdollar09.pdf . The 2009 investment grew by ...
Cached Biology News:Grant advances quark-gluon plasma studies
 2Grant advances quark-gluon plasma studies
 3In Parkinson's disease, brain cells abandon mitochondria, researchers report 2In Parkinson's disease, brain cells abandon mitochondria, researchers report 3US invested $139 billion in health research in 2009 2
Roll of Mesh 5m x 25cm...
Oven mesh sheets, large, 23 x 23cm, pack of 5...
... (PPase-2A) is a serine/threonine phosphatase isolated from ... as the heterodimer of 60kDa (A) and ... to dephosphorylate the a-subunit of phosphorylase kinase. ... broad substrate specificity and may play a ...
Immulon 2 HB 1x8 Strip assembled, C-bottom; high binding irradiated surface to provide increased binding affinity for hydrophilic proteins and complexes....
Biology Products: