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Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

analyzed on each Experion chip displayed an r2 value of 0.98. A representative plot of the data from one Pro260 analysis is shown in Figure 4. Though plots of the data from the same samples run on the bioanalyzer system also yielded an average r2 of 0.98, the protein 200 plus assay did not detect the 2.5 ng/l peak or clearly distinguish the 10 ng/l peak; therefore, graphs drawn from these data covered the smaller 502,000 ng/l range rather than the full range tested.

Sensitivity
The sensitivity, or limit of detection, of each system is also an important performance specification and is one factor that distinguishes the two automated electrophoresis systems.

Sensitivity becomes particularly important when protein concentrations are low or when purity is being evaluated. The separation of the E. coli lysate on both systems (Figure 2) illustrates that the Experion system is more sensitive than the bioanalyzer; when an identical sample was loaded onto both systems, the peaks in the Experion electropherogram were of a much higher intensity than those in the bioanalyzer electropherogram.

The sensitivity of both systems was also evaluated by comparing the signal-to-noise ratio produced by a dilute sample of CA (2.5 ng/l). The peak generated by this sample was not identified by the protein 200 plus assay (Figure 5); however, based on the peak height generated on the Experion system, a mean signal-to-noise ratio of 20.5 was calculated. The electropherogram shown in Figure 5A illustrates the extent to which the fluorescence signal associated with this CA sample rises above the background with the Experion system. The low detection limit of the Experion Pro260
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