Navigation Links
Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

e was separated by SDSPAGE on 420% gradient gels (data not shown). On the other hand, the protein 200 plus assay detected an average of only 23 protein peaks from the same sample. Representative electropherograms are shown in Figure 2 to demonstrate the superior resolving capabilities of the Experion system.

The resolution of two symmetrical peaks can also be defined using a resolution value, Rs, which is defined by the equation Rs = 1.17(t2 t1) /(w1 + w2), where t1 and t2 = migration times of the protein peaks, and w1 and w2 = widths of the peaks at half height. Using this equation, Rs > 1.5 indicates that two adjacent peaks are baseline resolved.

To calculate the Rs that might theoretically be achieved with both systems, we used the migration time and peak width data collected from the Pro260 ladder separations as reference points. For this analysis, the Rs achievable by each system was calculated for each ladder peak (within each systems respective separation range) and a hypothetical protein 10% larger in MW but producing a peak of the same shape. Experion software exported the migration times and peak width data necessary for solving the equation; however, these data had to be manually determined for the bioanalyzer. Note that resolution numbers obtained were theoretical and that, in reality, the apparent resolution for chip separation is dependent on and limited by several factors. Such factors include the heterogeneity of a protein population, and modification of proteins in the sample, which can cause peak broadening and can alter protein migration.

The
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:1/15/2014)... (PRWEB) January 15, 2014 The Microcompetition with ... major disease. One of these latent viruses is the Epstein ... arthritis (RA). Rheumatoid arthritis (RA) is a chronic inflammatory disease ... a study found that RA patients have high concentrations of ...
(Date:1/15/2014)... 2013 was a banner year of continued ... saw continued independent research led by the team at ... $1 million grant from the Susanne Marcus Collins Foundation, ... peer reviewed journal, Amy Grant highlighted Brainwave Optimization® in ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 AudioNotch is ... new therapy for the treatment of tinnitus. Patients listen ... frequency, and over a period of weeks to months, their ... Therapy in two forms: Notched Music and Notched White Noise. ...
(Date:1/14/2014)... PA (PRWEB) January 14, 2014 Date: Friday, ... , Location: Warrington Country Club, 1360 Almshouse Road, Warrington, Pa. ... national nonprofit organization solely dedicated to finding a cure for ... affected worldwide, will host its annual Crystal Ball on Friday, ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2
... Global Business Potential, SOUTH SAN FRANCISCO, Calif., ... company, today announced that it has been,selected by ... Private Companies for,2007. The first-annual elite GoingGreen 100 ... order to be eligible for the GoingGreen 100,list, ...
... JOLLA, Calif., Sept. 11 TorreyPines Therapeutics,Inc. (Nasdaq: ... Kurtz, President and,Chief Executive Officer of TorreyPines, will ... Tuesday, September 18th at 2:30 p.m., local,time (5:30 ... presentation will be available live. You can,access the ...
... Induce and Monitor Humoral and Cellular ... LEIDEN, The Netherlands, September 11 A new ... conducted by a,multi-member Dutch consortium with expertise in the ... the project is to develop,novel strategies to induce and ...
Cached Biology Technology:Solazyme, Inc. Selected by AlwaysOn as a GoingGreen 100 Top Private Company Award Winner 2Solazyme, Inc. Selected by AlwaysOn as a GoingGreen 100 Top Private Company Award Winner 3A Large-Scale Research Project by a Dutch Consortium Addressing a Global Problem 2A Large-Scale Research Project by a Dutch Consortium Addressing a Global Problem 3A Large-Scale Research Project by a Dutch Consortium Addressing a Global Problem 4A Large-Scale Research Project by a Dutch Consortium Addressing a Global Problem 5
(Date:4/17/2014)... such as heart disease, epilepsy and pain, making them ... is still much scientists do not know about the ... into the structure of sodium channels and, specifically, one ... responsible for ,fine-tuning, the activity of the channel. The ... the Journal of Biological Chemistry . , Nerves ...
(Date:4/17/2014)... in the Cell Press journal Current Biology ... rather novel sex lives. The Brazilian insects, which represent ... Neotrogla , are the first example of an animal ... been identified in several different animals, Neotrogla ... is also reversed," says Kazunori Yoshizawa from Hokkaido University ...
(Date:4/17/2014)... whereby the genetic information of DNA is used ... have numerous different functions in living organisms. Messenger ... gene expression, by relating the genetic information of ... proteins. , By examining the different types ... organism at a given time, researchers can determine ...
Breaking Biology News(10 mins):Structure of sodium channels different than previously believed 2In sex-reversed cave insects, females have the penises 2Rapid and accurate mRNA detection in plant tissues 2
... researchers at Cincinnati Children,s Hospital Medical Center has developed ... that often afflict hospitalized patients who receive central lines. ... Cincinnati Children,s, and her team developed the antibody, which ... thereby stopping the formation of biofilm in a rat ...
... the El Nio Southern Oscillation (ENSO), altering the cycles of ... and flooding to Australia and many other Pacific-rim countries., New ... from Kiribati has revealed how the ENSO cycle has changed ... changes have an impact on the strength and timing of ...
... winners of its 2013 awards. The awards will be presented ... Society of America in Austin, TX from November 10-13, 2013. ... OF HONOR This award is the highest award presented by ... attained preeminence in the field through outstanding contributions. Dr. John ...
Cached Biology News:Global warming could change strength of El Niño 2Entomological Foundation announces 2013 Insect Science Award winners 2Entomological Foundation announces 2013 Insect Science Award winners 3Entomological Foundation announces 2013 Insect Science Award winners 4Entomological Foundation announces 2013 Insect Science Award winners 5Entomological Foundation announces 2013 Insect Science Award winners 6Entomological Foundation announces 2013 Insect Science Award winners 7Entomological Foundation announces 2013 Insect Science Award winners 8Entomological Foundation announces 2013 Insect Science Award winners 9Entomological Foundation announces 2013 Insect Science Award winners 10Entomological Foundation announces 2013 Insect Science Award winners 11
... This CLS number is ... created to easily match ... showing no availability yet, ... old Sigma-Aldrich number (Z71,448-8) ...
A convenience pack containing one each of the most popular sizes of Cat. No. 4980 flask. Designed for the low volume user, a case contains one each of five sizes: 50mL, 125mL, 250mL, 500mL, and 1L....
Narrow-mouth has heavy-duty rim. Approximate graduations are in durable white enamel. Extra large marking space. capacity 125 mL Special grade: student grade...
... number is a new product ... Cornings product number. If showing ... under the old Sigma-Aldrich number ... for assistance. ID clarifier: barcoded ...
Biology Products: