Navigation Links
Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

tive protocol exists for their quantitation. By loading a chip with a series of known concentrations of a purified protein, the user can generate a calibration curve. An equation derived from the linear fit of the data points comprising this curve is then used to quantitate each protein in the other sample wells. This form of absolute quantitation performs best when the protein used for calibration is the same as that undergoing quantitation.

We applied this absolute quantitation approach using both microfluidic systems to see if the quantitation accuracy for CA could be improved. The software of each system was directed to generate a calibration curve from a dilution series, and then use a linear fit of the data to quantitate two concentrations of CA. Using this approach, accuracy of quantitation by the Pro260 assay was significantly improved, to within 15.8% of the UV-determined concentration, and the protein 200 plus assay, to within 10.5% (Table 2).

Resolution
Another important parameter that defines the overall performance of a protein separation system is its capacity for resolving one protein from another. Identification and quantitation improve as a protein of interest is separated to a greater degree from neighboring proteins.

To evaluate the resolution capabilities of each automated electrophoresis system, a 2 mg/ml E. coli protein lysate was separated with the Pro260 and protein 200 plus LabChip kits, and the total number of peaks detected by the software was recorded. An average of 35 protein peaks were identified with the Pro260 assay upon separation of this complex protein sample; essentially the same number of protein bands were observed when the same sampl
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:9/1/2015)... , Sept. 1, 2015   Medfusion, Inc., ... the relationship between doctors and patients, today announced ... Durham, N.C. -based venture capital firms.   ... business association with Bull City Venture Partners and ... Medfusion Founder and Executive Chairman. "Both firms, experience ...
(Date:9/1/2015)... ... September 01, 2015 , ... GenoCAD, a leading provider ... version of its flagship DNA design platform, which has been enhanced to boost ... numerous genetic parts, which in turn can be recombined using specific sets of ...
(Date:8/31/2015)... ... August 31, 2015 , ... In ... its properties such as low carbon footprints and compostability. These properties can help ... based plastics. On the basis of applications, the market for biodegradable plastics can ...
(Date:8/31/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/53wxp3/israel_biomedical ) ... Sensors Market - Growth, Trends & Forecasts (2014-2020)" ... Biomedical Sensors market is estimated at $0.19 billion by ... 2014-2020 The near future will bring biomedical ... each individual. These sensors can be set to trigger ...
Breaking Biology Technology:Medfusion Secures Funding to Continue Evolution and Advance Mobile Strategy 2Medfusion Secures Funding to Continue Evolution and Advance Mobile Strategy 3GenoCAD Releases Combinatorial Design Tool to Optimize Protein Expression and Streamline DNA Synthesis Orders 2Biodegradable Plastics Market Growing at 10.8% CAGR to 2020 2Biodegradable Plastics Market Growing at 10.8% CAGR to 2020 3Biodegradable Plastics Market Growing at 10.8% CAGR to 2020 4Biodegradable Plastics Market Growing at 10.8% CAGR to 2020 5Israel Biomedical Sensors Market 2015 - Growth, Trends & Forecasts to 2020 2
... ABBOTT PARK, Ill. and SAN DIEGO , ... ) and Neurocrine Biosciences, Inc. (Nasdaq: NBIX ) ... agreement to develop and commercialize elagolix for the treatment ... gonadotropin-releasing hormone (GnRH) antagonist, which has recently completed a ...
... , ... The mother of a previously healthy 20 year old woman found dead four ... United States Supreme Court case where the Court has been asked to bar ... the Court were to bar such lawsuits, manufacturers would have a lessened incentive ...
... 11, 2010 Researchers at Texas A&M University College of ... with the successful delivery of a foal using oocytes from ... The delivery of the foal highlights Texas A&M,s long tradition ... great experience for the owner of the new foal., "I,ve ...
Cached Biology Technology:Abbott and Neurocrine Announce Global Agreement to Develop and Commercialize Elagolix for the Treatment of Endometriosis 2Abbott and Neurocrine Announce Global Agreement to Develop and Commercialize Elagolix for the Treatment of Endometriosis 3Abbott and Neurocrine Announce Global Agreement to Develop and Commercialize Elagolix for the Treatment of Endometriosis 4Abbott and Neurocrine Announce Global Agreement to Develop and Commercialize Elagolix for the Treatment of Endometriosis 5Supreme Court Amicus Brief Filed Opposing Lawsuit Protection for Manufacturers of Defectively Designed Vaccines 2Texas A&M veterinary researchers achieve cloning first 2Texas A&M veterinary researchers achieve cloning first 3
(Date:8/19/2015)...  VOXX International Corporation (Nasdaq: VOXX ) ... Definitive Agreement to purchase the assets, inclusive of ... EyeLock through an acquiring entity.  Upon consummation of ... in the acquiring entity. The closing of the ...  Expanding on its existing supply chain and distribution ...
(Date:8/12/2015)... 12, 2015 As the digital ... innovation and advanced biometrics technology is accelerating beyond expectations.  ... rely on using their credit cards, the digital currency, ... for payment services led by companies in the space ... Google, Inc. (NASDAQ: GOOG ), Apple Inc. (NASDAQ: ...
(Date:8/10/2015)... The latest 364 page report from business intelligence provider ... border security market . Visiongain assesses that this ... Now: Border security is hard to achieve and harder to ... you need to know about - and more importantly, you ... this will impact your company and the border security industry ...
Breaking Biology News(10 mins):VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 2VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 3VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 4VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 5Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 2Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 3Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 4Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 5Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 6Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 2Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 4Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 5Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 6Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 7Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 8Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 9
... REDWOOD CITY, Calif., Dec. 2 DigitalPersona, Inc., a ... an agreement with IBM to deploy a new model ... 500 retail systems. The DigitalPersona U.are.U® 4500HD offering allows ... so that specific transactions can be linked to the ...
... of carbon about a nanometer in diameterhave been highly ... wires in molecular devices, or hydrogen storage components for ... at the National Institute of Standards and Technology (NIST) ... to the list: detection and destruction of an aggressive ...
... at the University of Southampton,s School of Ocean and ... (NOCS) has formally described four new species of king ... the new species in the Smithsonian Collections of the ... significance of the find, she said: "King crabs include ...
Cached Biology News:DigitalPersona Fingerprint Sensor Technology Ships on IBM SurePOS 500 Retail Systems 2Combining nanotubes and antibodies for breast cancer 'search and destroy' missions 2King crab family bigger than ever 2
Mouse polyclonal antibody raised against a partial recombinant MHC2TA. NCBI Entrez Gene ID = 4261...
... Major Science Smart Minis 300V power ... with ever higher voltage, sufficient for SDS-PAGE, ... specifications ensure two pairs of terminator pairs ... Supply is designed for individuals to have ...
... NightOWL is a dedicated imaging system ... size. Equipped with a very sensitive camera ... or plants in tumor research, infectious disease ... Western blots. NightOWL is the only imager ...
Mouse polyclonal antibody raised against a partial recombinant IFNA13. NCBI Entrez Gene ID = 3447...
Biology Products: