Navigation Links
Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

tive protocol exists for their quantitation. By loading a chip with a series of known concentrations of a purified protein, the user can generate a calibration curve. An equation derived from the linear fit of the data points comprising this curve is then used to quantitate each protein in the other sample wells. This form of absolute quantitation performs best when the protein used for calibration is the same as that undergoing quantitation.

We applied this absolute quantitation approach using both microfluidic systems to see if the quantitation accuracy for CA could be improved. The software of each system was directed to generate a calibration curve from a dilution series, and then use a linear fit of the data to quantitate two concentrations of CA. Using this approach, accuracy of quantitation by the Pro260 assay was significantly improved, to within 15.8% of the UV-determined concentration, and the protein 200 plus assay, to within 10.5% (Table 2).

Resolution
Another important parameter that defines the overall performance of a protein separation system is its capacity for resolving one protein from another. Identification and quantitation improve as a protein of interest is separated to a greater degree from neighboring proteins.

To evaluate the resolution capabilities of each automated electrophoresis system, a 2 mg/ml E. coli protein lysate was separated with the Pro260 and protein 200 plus LabChip kits, and the total number of peaks detected by the software was recorded. An average of 35 protein peaks were identified with the Pro260 assay upon separation of this complex protein sample; essentially the same number of protein bands were observed when the same sampl
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:8/19/2014)... DUBLIN , Aug. 19, 2014 Research and ... Culture Supplies - Global Strategic Business Report" report to their ... Cell and Tissue Culture Supplies in US$ Thousands by the following ... separate comprehensive analytics for the US, Canada , ... Asia-Pacific , and Latin America . ...
(Date:8/19/2014)... , Aug. 19, 2014 Research and ... Fuel Cells (SOFCs) - Global Strategic Business Report" report ... worldwide markets for Solid Oxide Fuel Cells (SOFCs) in US$ ... Canada , Europe , ... estimates and forecasts are provided for the period 2012 through ...
(Date:8/19/2014)... 2014 Research and Markets ... "Spectroscopy Equipment and Accessories - Global ... offering. This report analyzes ... Accessories in US$ Thousands by the following ... Spectrometers, NMR-EPR Spectrometers, Near Infrared (NIR) Spectrometers, ...
(Date:8/19/2014)... 19, 2014   Synthetic Biologics, Inc. ... anti-infective biologic and drug programs targeting specific pathogens ... that its novel C. difficile development ... Interscience Conference on Antimicrobial Agents and Chemotherapy ... Synthetic Biologics, Senior Vice ...
Breaking Biology Technology:Global Cell and Tissue Culture Supplies - Strategic Business Report 2014 2Global Solid Oxide Fuel Cells (SOFCs) - Strategic Business Report 2014 2Global Spectroscopy Equipment and Accessories - Strategic Business Report 2014 2Synthetic Biologics Announces Late-Breaking Poster Presentation for C. difficile Program at 54th ICAAC 2Synthetic Biologics Announces Late-Breaking Poster Presentation for C. difficile Program at 54th ICAAC 3Synthetic Biologics Announces Late-Breaking Poster Presentation for C. difficile Program at 54th ICAAC 4
... Almost by accident, I find myself on what must surely ... the past few months, I have tried the best designs ... I've tried has great strengths and a few tradeoffs, usually ... along comes a Bluetooth wireless headset from a newcomer to ...
... major advantages , ,MADISON, Wis. Wisconsins biotechnology ... feature appearing in the June issue of Modern Drug ... clusters in Madison and Milwaukee, and attributes the growth ... and universities, the Wisconsin Alumni Research Foundation , ...
... lack of funding cited as problems , ,MADISON ... and keep customers happy isnt exactly a revolutionary practice. ... lack funding, even the smallest technological alteration can be ... Matt Miszewski and other speakers described this paradox and ...
Cached Biology Technology:The quest for the perfect headset 2The quest for the perfect headset 3Wisconsin biotech sector featured in scientific magazine 2Wisconsin biotech sector featured in scientific magazine 3State CIO discusses IT and government 2
(Date:8/20/2014)... Aug. 20, 2014 /CNW/ -  Issue ... can quickly become life threatening for some children.    ... 1.2 million Canadians have food allergies and this number may ... of children are estimated to have food allergies.  ... skin irritations and hives to breathing difficulties and loss of ...
(Date:8/20/2014)... Area Lyme Foundation, which aims to make Lyme disease ... research published in an upcoming issue of the Elsevier ... The findings show that ticks that carry Lyme ... making the threat of Lyme disease year-round. The research ... Health (CDPH) Vector-borne Disease Section and University of California, ...
(Date:8/20/2014)... sunscreen and seawater signals a relaxing trip to the ... beach vacation comes with an environmental hitch. When certain ... they can become toxic to some of the ocean,s ... other marine animals. Their study appears in the ACS ... Tovar-Sanchez and David Snchez-Quiles point out that other than ...
Breaking Biology News(10 mins):Information Update - Allergens and food safety at school - What's in your child's lunch? 2Information Update - Allergens and food safety at school - What's in your child's lunch? 3Lyme disease risk is year-round in Northwest California, according to new study 2Lyme disease risk is year-round in Northwest California, according to new study 3
... Wisconsin research team, led by John W. Lough, Ph.D., professor ... stem cells (ES cells) in animals can be cultivated to ... to replace tissue damaged as a result of a heart ... or diseased cells in adult tissues has caused extraordinary interest ...
... Durham, N.C. ?A new understanding of the causes for symptoms ... 600 African-Americans, has resulted from a study by researchers at ... Their findings may lead to a new, more direct method ... that an inability of red blood cells to relax blood ...
... each year worldwide, causing one million deaths. Around 90% ... children. One of the greatest challenges facing Africa in ... chloroquine (CQ), the cheapest and most widely used antimalarial, ... the first-developed and least expensive alternative to CQ, is ...
Cached Biology News:Stem Cell Research Shows Potential for Replacing Tissue Damaged in Heart Attacks 2Discovery Promises Simpler Therapy for Sickle Cell Disease 2Discovery Promises Simpler Therapy for Sickle Cell Disease 3Reducing malarial transmission in Africa 2
Recommend to use at up to 5000X dilutions for cell culture (e.g. Add 1 ul to 1 ml of culture medium.)...
... chromatographically purified from egg white. The extinction coefficient ... is in 10 mM phosphate-buffered saline, ... 0.05% sodium azide and 50% glycerol. Avidin (from ... is purified by molecular sieving and ...
... Blunt TOPO PCR Cloning ... designed for cloning and ... PCR products. The pCR4Blunt-TOPO ... Cloning and greater than ...
Recommend to use at 1000X dilutions for cell culture (e.g. Add 1 ul to 1 ml of culture medium.)...
Biology Products: