Navigation Links
Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

otein 200 plus assay and bioanalyzer was less reproducible (CV of 19.0% and 18.2%) for the same two sample preparations. To further illustrate the difference in quantitation reproducibility for the two systems, all data points acquired for BSA quantitation were plotted (Figure 1). The clustering of the Pro260 assay data indicates that the interchip and intrachip reproducibility of quantitation by this assay was superior to that observed with the protein 200 plus assay.

For both protein analysis systems, the accuracy of relative quantitation was much greater for the BSA sample than for CA. The Pro260 assay produced deviations in quantitation of approximately 7% and 71% for BSA and CA, respectively, and the protein 200 plus assay quantitated these same two protein samples to within approximately 20% and 48% of the spectroscopically measured concentration (Table 2).

Such differences in quantitation accuracy for different proteins also occur with other commonly used protein quantitation methods, such as the Bradford and Lowry assays (bulletin 1069, Bradford 1976, Lowry et al. 1951), and are often due to differences in protein staining efficiency. Unique protein characteristics, such as primary sequence, amino acid composition, isoelectric point, and presence of modified amino acids and prosthetic groups, can affect the levels of staining and colorimetric signals produced. Both microfluidic systems perform quantitation using a single-point calibration to an internal upper marker protein; thus, relative quantitation accuracy is often highest for proteins that stain with similar efficiency to the upper marker protein.

Because proteins exhibit differences in staining behavior, an alterna
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:11/22/2014)... Nov. 21, 2014  The element of surprise has ... i.e., they strike without warning. Until now, epidemiologists had ... a viral outbreak, resulting in suboptimal responses to both ... Photo - http://photos.prnewswire.com/prnh/20141121/160242 ... been removed because virus gene structural changes have been ...
(Date:11/21/2014)... Quebec , November 21, 2014 ... como responsable comercial   Mariano Rodríguez es ...   KLOX está en marcha para comenzar ... de cura de heridas de reciente aprobación en Europa   ... "la compañía") se complace al anunciar los siguientes nombramientos: ...
(Date:11/21/2014)... (PRWEB) November 19, 2014 The science ... lives was shared with more than 200 primary students ... students from the University of Otago Physics Department and ... The Otago students, members of the university’s OSA/SPIE Student ... in early September, giving children from small, rural schools ...
(Date:11/21/2014)... 20, 2014 Author Matthew J. Pallamary’s ... the Other Side” published as a tribute to ... the “Fiction: Short Story” category of the 2014 ... and CEO of USA Book News, said this year’s ... publishers, including Simon & Schuster, Penguin, John Wiley & ...
Breaking Biology Technology:Viruses' Advantage of Surprise is Lost 2KLOX Technologies anuncia sus nombramientos ejecutivos 2KLOX Technologies anuncia sus nombramientos ejecutivos 3KLOX Technologies anuncia sus nombramientos ejecutivos 4KLOX Technologies anuncia sus nombramientos ejecutivos 5Optics and photonics outreach programs share science, spark fascination with light 2Optics and photonics outreach programs share science, spark fascination with light 3Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 2Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 3Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 4
... www.WhatWillYourPlanBe.com offers post college-aged women expert guidance in ... 17 Many young women enter the "real ... plan, particularly when it comes to their careers, ... that the unexpected often happens -- a looming ...
... Sales Grow by 10.7% to EUR1.06bn, - Adjusted Earnings per Share ... - Adjusted EBITDA Margin Rises From 19.0 to 19.5%, ... again considers itself well equipped to meet the global challenges in, ... of 6% to 7% in, the core business.", , ...
... test aids in identifying connection between improved lipid profile ... Using the VAP Cholesterol Test from ... found an association between a rare gene mutation and ... could help in the development of heart disease risk-lowering ...
Cached Biology Technology:Helping Young Women Succeed in Today's Unpredictable World 2Helping Young Women Succeed in Today's Unpredictable World 3Helping Young Women Succeed in Today's Unpredictable World 4Following a Record Year in 2008 Gerresheimer Again Sets its Sights on Substantial Growth in 2009 2Following a Record Year in 2008 Gerresheimer Again Sets its Sights on Substantial Growth in 2009 3Following a Record Year in 2008 Gerresheimer Again Sets its Sights on Substantial Growth in 2009 4VAP Cholesterol Test Helps In Search For New Heart Disease Drugs 2VAP Cholesterol Test Helps In Search For New Heart Disease Drugs 3VAP Cholesterol Test Helps In Search For New Heart Disease Drugs 4
(Date:11/21/2014)... 2014  Earlier this year Donald Spector , ... one of the most prolific inventors in the world, ... from Smartphones to third party agencies. Spector envisioned this ... of the earliest known patents in this area. Now ... military, child care, elder care and hospital applications. This ...
(Date:11/21/2014)... , Nov. 20, 2014 C-Labs LLC, a ... Internet of Things (IoT), today announced the appointment of ... operating officer. Previously a strategic advisor to the firm, ... operations. Mr. Traynor is based out of the C-Labs ... reports to Chris Muench , Chief Executive Officer. ...
(Date:11/18/2014)... WASHINGTON , Nov. 18, 2014  The ... Security Industry Association today jointly announce the formation ... and release of its Identity and Biometric ... (Framework). The Framework received official support from BORDERPOL, ... governments to improve and provide expertise regarding border ...
Breaking Biology News(10 mins):Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4C-Labs Names Former Microsoft and Bsquare Executive as Chief Operating Officer 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3
... in the world, but only a handful are responsible ... in agricultural damage globally every year. Nematodes are small ... living cells. Now, Iowa State University researchers have ... one of the most destructive nematodes: Meloidogyne incognita ...
... Sept. 4, 2008 As the anniversary of the ... has issued a new podcast describing an array of ... The podcast is the sixth episode in ACS,s ... episodes posted twice monthly, Global Challenges examines daunting problems ...
... including researchers at the HudsonAlpha Institute and Stanford ... other organizations, today publishes a comprehensive analysis of ... results are the first from the Cancer Genome ... by the National Cancer Institute and the National ...
Cached Biology News:ISU researchers help map first plant-parasitic nematode genome sequence 2New 'chemical radar' among national security innovations in ACS podcast 2New 'chemical radar' among national security innovations in ACS podcast 3Collaboration between researchers yields more comprehensive portrait of brain cancer 2
...
Mouse polyclonal antibody to PRDM2 - PR domain containing 2, with ZNF domain...
Anti Human S14G-Humanin Polyclonal Antibody...
... interface for data capture, recording, manipulation, ... from paper to this powerful solution ... an integrated LIMS, notary service, customer ... functions allow LABTrack to achieve in ...
Biology Products: