To further emphasize the minimal variance of the Pro260 analysis in the presence of higher salt concentrations, data from individual chips were plotted (Figure 6). Evident from the plot are the consistent CA concentration determinations and clustering of the data points for the Pro260 analysis at all three salt concentrations.
Overall, the Experion Pro260 analysis was equivalent to or even superior to the protein 200 plus assay in every perfor`mance category examined. The Pro260 analysis displayed a broader separation range, allowing for the analysis of a larger population of proteins, and delivered accurate and reproducible sizing and relative quantitation of proteins, even in the presence of high concentrations of salt.
Accurate absolute quantitation was also achieved using the internal calibration curve function in the Experion software. Moreover, the Experion system provided enhanced resolution capabilities and up to 4-fold higher sensitivity, permitting the separation and detection of more sample proteins. The Experion system also provided a linear response over a concentration range of nearly three orders of magnitude, giving greater flexibility with sample loads. Taken together, the integrated, highquality components of the Experion system and Pro260 analysis kit offer an automated platform that provides superior data quality for a variety of protein applications.
Bio-Rad bulletin 1069, Colorimetric protein assays
Bradford MM, A rapid and sensitive method f