Navigation Links
Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

Marie Nguyen and William Strong, Bio-Rad Laboratories, Inc., 6000 James Watson Drive, Hercules, CA 94547 USA


Introduction
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is an established technique employed for protein separation. However, this traditional gel-based method involves multiple manual steps and several hours to complete. New microfluidics-based technologies, such as the LabChip platform developed by Caliper Life Sciences, Inc., have advanced protein separations by miniaturizing and automating the time-consuming separation, staining, imaging, and analysis steps required to complete the SDS-PAGE process. Microfluidic chip-based automated electrophoresis systems, such as the Experion system and the Agilent 2100 bioanalyzer, utilize the LabChip platform to perform SDS-PAGE-like separation and analysis of protein samples.

The Experion system uses the Experion Pro260 analysis kit for the separation of 10260 kD proteins under denaturing conditions. Typically, a Pro260 separation and analysis of up to ten samples is accomplished in approximately 30 min. This automated system can be used to perform a variety of tasks, such as monitoring purification procedures (by measuring the concentration and checking the purity of protein fractions) and performing one-dimensional differential expression studies, degradation assessments, cleavage investigations, and analysis of immunoprecipitation experiments.

This tech note presents a comparison of the performance of the Experion Pro260 analysis kit with the Agilent protein 200 plus LabChip kit, which is employed by the 2100 bioanalyzer, for protein analysis. Specifically, the accuracy and reproducibilit
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:8/31/2014)... Available spectra is similar for all L-series products ... a wide range of plants. The spectra is ... and pending wide spectrum technology. The single chip ... in contrast to growth spectra made from monochromatic ... large variety of differently sized and shaped growing ...
(Date:8/29/2014)... but one of the most promising technologies for future ... light (photons) instead of electrons. First, it is necessary ... photons and control their direction. Researchers around the world ... control, but now scientists at the Niels Bohr Institute ... emitted one at a time and in a particular ...
(Date:8/29/2014)... NY (PRWEB) August 29, 2014 Local ... Hills Veterinary Hospital are seeking candidates to participate in ... with osteoarthritis. The ultimate goal of this study ... stem cells into one or two arthritically affected joints ... joints. , Candidates for the current investigational study ...
(Date:8/28/2014)... SPIE Fellow Glenn Boreman, Professor and ... and Director of the Center for Optoelectronics and Optical ... and co-founder and Chairman of the Board of Plasmonics, ... Vice President of SPIE, the international society for optics ... recent election results at the Annual General Meeting of ...
Breaking Biology Technology:Breakthrough in light sources for new quantum technology 2Burnt Hills Veterinary Hospital Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2Glenn Boreman, UNC Professor and Plasmonics Co-Founder, Elected to SPIE Presidential Chain 2
... Md., May 4 Meso Scale Diagnostics, LLC. (MSD) ... in detecting the first identified case of infection with ... of Care Influenza Test, which is being developed under ... Prevention (CDC), is the investigational device referred to in ...
... Circ Pharma Limited ("Circ Pharma") and,Elan Corporation ... into a,Development Agreement to develop a chronotherapeutic formulation ... severe pain. , The ... once daily dosing. Circ,s technology provides for,minimized delivery ...
... Corporation, an orthopedic implants company focused on silicon ... received a CE (Conformite Europeene) mark for initial ... CSC (cancellous-structured ceramic) technology. The CSC technology, ... a porous cancellous trabecular structure designed to promote ...
Cached Biology Technology:Meso Scale Diagnostics' Point of Care Influenza Test Led to the First Identification of A(H1N1) Swine Flu Infection 2Circ Pharma Limited Enters Into Development Agreement for Chrono Tramadol With Elan Corporation Plc 2Amedica Receives CE Mark for Valeo(TM) Ceramic Spinal Implant System Incorporating CSC Technology 2
(Date:8/31/2014)... are not busy attacking us, germs go after each ... spell disaster for the infected microbes: Sometimes viruses actually ... say, expand its diet or better attack its own ... an immune system would robotically destroy anything it recognized ... University have now revealed that one variety of the ...
(Date:8/29/2014)... some surprising discoveries about the body,s initial responses ... the team found that specialized cells in the ... viral invasion and are the source of gut ... (IL-1β). , Though aimed at the presence ... epithelium that provides a barrier to protect the ...
(Date:8/29/2014)... number of connections in transcription factor networks (TFNs) to ... study published in PLOS Computational Biology in ... contributes to a network,s resilience against mutations. , ... of TFN models has a greater effect on robustness ... assortativity," said Dov A. Pechenick, PhD, lead author and ...
Breaking Biology News(10 mins):Discovery reveals how bacteria distinguish harmful vs. helpful viruses 2Discovery reveals how bacteria distinguish harmful vs. helpful viruses 3The early cost of HIV 2Assortativity signatures of transcription factor networks contribute to robustness 2
... Advanced Technology Microwave Sounder (ATMS) on board NASA,s newest Earth-observing ... The image shows the ATMS channel 18 data, which measures ... visible in the data, as the patch of blue, in ... The data were processed at the NOAA Satellite Operations Facility ...
... floating weed that clogs waterways around the world has at ... intended for boats and submarines. The Brazilian fern ... in part because its surface is dotted with oddly shaped ... stay afloat. In the November 1 issue of ...
... of African elephants than habitat, and human conflict in particular has ... new University of British Columbia study published online in PLoS ... to date, the study shows the elephant population in the Okapi ... in the Democratic Republic of Congo (DRC) saw a 50 ...
Cached Biology News:NASA'S NPP satellite acquires first ATMS measurements 2Plant with 'eggbeater' testure inspires waterproof coating 2Plant with 'eggbeater' testure inspires waterproof coating 3More than 50 percent decline in elephants in eastern Congo due to human conflict: UBC research 2
... system designed for flexibility and routine operation. ... ion lens, true hyperbolic quadrupole and high-speed ... widest range of sample types and applications. ... as standard Agilents unique ShieldTorch System, which ...
... The LUMIstar OPTIMA is a ... is designed for the widest possible ... labels: Reporter gene assays luciferase, ... (luminol, acridinium esters, dioxetanes..) Luminescent ...
... Quidel's depleted sera are specifically depleted of ... of the depleted protein, the alternative and ... are therefore ideal for the detection and ... sera have been used to assess complement ...
... The latest generation of the ... M.I.T. for the iontophoresis of neural ... (HRP) or tritiated amino acids into ... Stoelting. It may also be used ...
Biology Products: