Navigation Links
Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

Marie Nguyen and William Strong, Bio-Rad Laboratories, Inc., 6000 James Watson Drive, Hercules, CA 94547 USA


Introduction
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is an established technique employed for protein separation. However, this traditional gel-based method involves multiple manual steps and several hours to complete. New microfluidics-based technologies, such as the LabChip platform developed by Caliper Life Sciences, Inc., have advanced protein separations by miniaturizing and automating the time-consuming separation, staining, imaging, and analysis steps required to complete the SDS-PAGE process. Microfluidic chip-based automated electrophoresis systems, such as the Experion system and the Agilent 2100 bioanalyzer, utilize the LabChip platform to perform SDS-PAGE-like separation and analysis of protein samples.

The Experion system uses the Experion Pro260 analysis kit for the separation of 10260 kD proteins under denaturing conditions. Typically, a Pro260 separation and analysis of up to ten samples is accomplished in approximately 30 min. This automated system can be used to perform a variety of tasks, such as monitoring purification procedures (by measuring the concentration and checking the purity of protein fractions) and performing one-dimensional differential expression studies, degradation assessments, cleavage investigations, and analysis of immunoprecipitation experiments.

This tech note presents a comparison of the performance of the Experion Pro260 analysis kit with the Agilent protein 200 plus LabChip kit, which is employed by the 2100 bioanalyzer, for protein analysis. Specifically, the accuracy and reproducibilit
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
10. Highly Sensitive microRNA Array Performance
11. High Throughput RNA Isolation for High Performance Microarray Analysis
Post Your Comments:
(Date:3/30/2015)... , March 30, 2015 /CNW/ - A new ... on the salaries and demographic profiles of new graduates ... the Door – Building Careers for New Grads in ... Research-Based Pharmaceutical Companies (Rx&D) and features data ... Career Focus wage subsidy program from 2013 to 2015. ...
(Date:3/30/2015)... PARK, N.J. , March 30, 2015 /PRNewswire/ ... (PAMORA) under development by Shionogi & Co., Ltd., ... phase III study (COMPOSE I) for the treatment ... chronic non-cancer pain receiving opioid therapy. Study results ... daily) statistically significantly improved the frequency of spontaneous ...
(Date:3/29/2015)... , March 29, 2015 ... of important study data showing the presence ... 1,599 gynecologic cancer samples. Using Caris Molecular ... service, researchers identified expression of programmed cell ... independent of histology while its ligand (PD-L1) ...
(Date:3/27/2015)... , March 27, 2015  Neogen Corporation (NASDAQ: ... has adopted a prearranged trading plan in accordance with ... , Chairman and CEO of Neogen Corporation, is a ... Neogen Corporation common stock. Herbert does not ... was appropriate. The filing is only for a portion ...
Breaking Biology Technology:New Report on Graduates in Canadian Biotech Industry Sheds Light on Hiring Practices 2New Report on Graduates in Canadian Biotech Industry Sheds Light on Hiring Practices 3New Report on Graduates in Canadian Biotech Industry Sheds Light on Hiring Practices 4Shionogi & Co., Ltd. Announces That Naldemedine Meets Primary Endpoint In A Phase 3 Study For The Treatment Of Opioid-Induced Constipation 2Shionogi & Co., Ltd. Announces That Naldemedine Meets Primary Endpoint In A Phase 3 Study For The Treatment Of Opioid-Induced Constipation 3Caris Life Sciences' Multiplatform Tumor Profiling Technology Identifies Expression of PD-1 and PD-L1 in Gynecologic Malignancies 2Caris Life Sciences' Multiplatform Tumor Profiling Technology Identifies Expression of PD-1 and PD-L1 in Gynecologic Malignancies 3Caris Life Sciences' Multiplatform Tumor Profiling Technology Identifies Expression of PD-1 and PD-L1 in Gynecologic Malignancies 4Neogen CEO adopts 10b5-1 Trading Plan 2
... available in German . Probiotic ... However, the production of this "functional food" has its ... to survive conventional production processes. Researchers from Technische Universitaet ... allows the use of thus far unutilized probiotics. The ...
... the space shuttle Atlantis will streak skyward from the Kennedy ... the STS-135 flight marks the end of the space shuttle,s ... of research into infectious diseases, thanks to space bound experiments ... with their colleagues at Arizona State University,s Biodesign Institute. ...
... oldest known life-forms, but they are not well understood. ... microorganisms were designated as a domain of life distinct ... Robert Gunsalus, a UCLA professor of microbiology, immunology ... because of their ability to thrive in harsh ...
Cached Biology Technology:TUM researchers develop environmentally friendly process to improve storage stability of probiotics 2TUM researchers develop environmentally friendly process to improve storage stability of probiotics 3Final countdown: Atlantis to carry next-generation vaccine candidate on last space voyage 2Final countdown: Atlantis to carry next-generation vaccine candidate on last space voyage 3Final countdown: Atlantis to carry next-generation vaccine candidate on last space voyage 4Old life capable of revealing new tricks after all 2Old life capable of revealing new tricks after all 3
(Date:3/17/2015)... Minn. , March 17, 2015 ... technology company specializing in clinical study management systems, ... be in attendance at ARENA,s Outsourcing in Clinical ... NC.  This event provides an excellent forum for ... easy-to-use, flexible and affordable software-as-a-service (SaaS) eClinical technology ...
(Date:3/17/2015)... , March 17, 2015  MecklerMedia Corporation (OTCQX: ... exhibition for service robots ever held in ... place on May 11-13, 2015 at the Javits ... for the event include: Acorn Product Development; Axis ... Infranor; Kinova Robotics; Littler; NewBotic Corporation; Neya Systems ...
(Date:3/12/2015)... , March 12, 2015 IriTech, a ... a member of the Texas Instruments Design Network, announced ... has demonstrated its IriShield USB MK2120U device during ... facility for pension distribution. The iris ... to be marketed in India ...
Breaking Biology News(10 mins):Connect With MedNet Solutions At Outsourcing In Clinical Trials Southeast 2MecklerMedia's RoboUniverse New York Announces Sponsors and Exhibitors, May 11-13, 2015 2Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 2Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 3
... researchers is working to characterize the genes involved in wood ... quality and quantity of wood as a feedstock for biofuels ... important complement to the work of other biofuels researchers who ... for biofuels," said Eric Beers, professor of horticulture and the ...
... 2011 ID World 2011, the International Congress ... presented its ID People Trail Blazer Award to Peter Went, ... award was announced at the 2011 ID World International Congress ... de la Giroday. The Trail Blazer Award honors pioneering researchers ...
... German . For the first time, ... Research in Rostock (MPIDR) has calculated a profile that illustrates ... This profile applies to U.S. citizens, as data for this ... the analysis is universally valid, and can be applied to ...
Cached Biology News:Genomics of wood for biofuels production investigated 2Peter Went Wins ID People's Trail Blazer Awards 2Individual CO2 emissions decline in old age 2Individual CO2 emissions decline in old age 3Individual CO2 emissions decline in old age 4
... interferons are cytopathic effect inhibition assays, also known ... include; tissue culture supernatents; serum from PK studies ... unit is defined as the quantity of interferon ... of viral infection by 50%. All ...
... effect inhibition assays, also known as CPE and ... supernatents; serum from PK studies and delivery formulations. ... as the quantity of interferon required per milliliter ... by 50%. All standards used in ...
...
Request Info...
Biology Products: