Marie Nguyen and William Strong, Bio-Rad Laboratories, Inc., 6000 James Watson Drive, Hercules, CA 94547 USA
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is an established technique employed for protein separation. However, this traditional gel-based method involves multiple manual steps and several hours to complete. New microfluidics-based technologies, such as the LabChip platform developed by Caliper Life Sciences, Inc., have advanced protein separations by miniaturizing and automating the time-consuming separation, staining, imaging, and analysis steps required to complete the SDS-PAGE process. Microfluidic chip-based automated electrophoresis systems, such as the Experion system and the Agilent 2100 bioanalyzer, utilize the LabChip platform to perform SDS-PAGE-like separation and analysis of protein samples.
The Experion system uses the Experion Pro260 analysis kit for the separation of 10260 kD proteins under denaturing conditions. Typically, a Pro260 separation and analysis of up to ten samples is accomplished in approximately 30 min. This automated system can be used to perform a variety of tasks, such as monitoring purification procedures (by measuring the concentration and checking the purity of protein fractions) and performing one-dimensional differential expression studies, degradation assessments, cleavage investigations, and analysis of immunoprecipitation experiments.
This tech note presents a comparison of the performance of the Experion
Pro260 analysis kit with the Agilent protein 200 plus LabChip kit, which
is employed by the 2100 bioanalyzer, for protein analysis. Specifically,
the accuracy and reproducibilit