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Perform RNAi Library Screens on Any Budget

d to make Ambions Silencer CellReady siRNA Libraries. This ensures that the siRNAs are stable and easy to resuspend.

Figure 3. Optimizing Transfection with the Silencer CellReady siRNA Transfection Optimization Kit. (A) Experimental design of a transfection optimization experiment using the Silencer CellReady siRNA Transfection Optimization Kit. (B) Knockdown of GAPDH levels were determined after the experimenets were performed as described in (A). In only two days, conditions that effectively delivered siRNAs into two cell types (HepG2 and A549) were identified.


To use, simply add the desired amount of transfection agent (0.4 ml siPORT NeoFX siRNA Transfection Agent is supplied, but other transfection agents can also be used), briefly incubate, and then overlay with the desired number of cells. Since the positive control siRNA targets GAPDH, a well-studied housekeeping gene expressed in all human cell types and often used as a control, target mRNA levels can be readily monitored using assays that most researchers already have available in their laboratories. siRNA induced knockdown of GAPDH protein can also be very quickly and easily monitored by the new KDalert GAPDH Assay Kit, which assesses GAPDH enzymatic activity via a fluorescence assay. (This kit, which was developed specifically for use with the Silencer CellReady siRNA Transfection Optimization Kit and Silencer GAPDH siRNA
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