Navigation Links
Perform Microarray Analysis with Limited Bacterial RNA

not be compared directly with amplified samples. All amplification methods, or any labeling method for that matter, produce some biases in mRNA representation. What is important for expression analysis is that the biases are reproducible within a method and that similar labeling methods are used on samples being compared. MessageAmp II-Bacteria compares favorably with standard labeling protocols in terms of reproducibility and dynamic range, but with a dramatically reduced requirement for input RNA.


A Complete Kit for Bacterial RNA Amplification
The MessageAmp II-Bacteria Kit provides reagents for 20 RNA amplifications starting with as little as 10 ng bacterial mRNA or as much as 500 ng total RNA. A variety of labeling methods can be used with the kit, including 33P cDNA labeling, direct labeling with Cy dyes or biotin, and indirect coupling using aminoallyl nucleotides (modified nucleotides are not provided with the kit). E. coli total RNA is provided as a control.

While MessageAmp II-Bacteria can be successfully used with total RNA, MICROBExpress enriched bacterial mRNA is a much better choice for microarray analysis. A 10-fold to 100-fold increase in sensitivity is seen when using enriched mRNA, compared with total RNA. Overall signal and signal-to-noise ratios can be dramatically increased by using RNA prepared using the MICROBEnrich and/or MICROBExpress Kits with MessageAmp II-Bacteria [1].
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Performance Comparisons of Commercial RT-PCR Systems
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
8. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
9. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
10. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
11. Highly Sensitive microRNA Array Performance
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Perform Microarray Analysis with Limited Bacterial RNA

(Date:3/3/2015)... (PRWEB) March 03, 2015 GlobalStemCellsGroup.com ... growing faculty. Camacho Alcocer, a biomedical researcher and founding ... will join GSCG’s team of biomedical professors who will ... program. , Criogenix SA de CV is a private ... Technological Institutions and Companies in Mexico (RENIECYT). , Camacho ...
(Date:3/2/2015)... York, NY (PRWEB) March 02, 2015 ... Testing Services industry has experienced robust growth. The ... consumer products, industrial materials, energy resources and other goods, ... and rising government regulation. As a result, IBISWorld forecasts ... of 2.8% to reach $946.8 million over the five-year ...
(Date:3/2/2015)... and VANCOUVER, British Columbia , ... (CSE: IN; OTCQB: IMLFF), a clinical stage biopharmaceutical ... therapies, today announced that Craig Schneider , ... during a live webcast at VirtualInvestorConferences.com. ... http://VirtualInvestorConferences.com > click the red "register/ ...
(Date:3/2/2015)... MENLO PARK, Calif. , March 2, 2015 /PRNewswire/ ... company in the emerging field of regenerative medicine, today ... year 2014 financial and operating results on Tuesday, March ... markets. The Company will host a conference call and ... Eastern / 1:30 p.m. Pacific to discuss the results ...
Breaking Biology Technology:Mario Camacho Alcocer Joins Global Stem Cells Group Faculty 2Mario Camacho Alcocer Joins Global Stem Cells Group Faculty 3Laboratory Testing Services in Canada Industry Market Research Report Now Available from IBISWorld 2Laboratory Testing Services in Canada Industry Market Research Report Now Available from IBISWorld 3InMed Pharmaceuticals to Present Investor Webcast March 5 2Asterias Biotherapeutics to Report Fourth Quarter and Full Year 2014 Results on March 10, 2015 2
... Neurocrine Biosciences, Inc. (Nasdaq: NBIX ) ... Officer of Neurocrine Biosciences, will be presenting at the ...   The live presentation takes place on ... 8:00 a.m. Pacific Time.  The presentation will be webcast ...
... 21, 2012  Thought leaders from the medical community in Europe ... albumin in treating liver disease and sepsis, and in cardiac ... Dialogue – "Albumin in Clinical Fluid Management ." ... participants engaged in a frank and provocative exchange about the ...
... Addario Lung Cancer Medical Institute,s research portfolio is ... platform for venture philanthropy in medical research. The ... president and COO of ALCMI, sister foundation of ... (Logo:  http://photos.prnewswire.com/prnh/20120221/DC56587LOGO ) ...
Cached Biology Technology:Medical Experts Who Participated in CSL Behring's Key Issues Dialogue Prefer Albumin for Fluid Management 2Medical Experts Who Participated in CSL Behring's Key Issues Dialogue Prefer Albumin for Fluid Management 3FasterCures Spotlights Addario Lung Cancer Medical Institute's Innovative Research Programs 2FasterCures Spotlights Addario Lung Cancer Medical Institute's Innovative Research Programs 3
(Date:2/12/2015)... , Feb. 12, 2015 NXT-ID, Inc. (NASDAQ: ... "Company"), a biometric authentication company focused on the growing ... provisional patent 62/113114 for DISTRIBUTED METHOD AND ... The patent further establishes NXT-ID,s position in the ... property portfolio the ability for multiple devices to collaborate ...
(Date:2/5/2015)... , 5. Februar 2015 Marken ... Logistikunternehmen und hat eine neue Marketingkampagne gestartet, ... (Clinical Logistics Organization – CLO) der Branche ... lautet First , mit Schwerpunkt auf ... http://photos.prnewswire.com/prnh/20150205/173753 Logo - ...
(Date:2/5/2015)... 2015   Marvin Test Solutions , ... test solutions for military, aerospace, and manufacturing ... TS-323 GENASYS Test Platform to ... is a high-performance PXI-based system designed to ... require performance functional testing. GENASYS features a ...
Breaking Biology News(10 mins):NXT-ID Patents Collaborative Services to Enable the "Internet of Things" 2NXT-ID Patents Collaborative Services to Enable the "Internet of Things" 3Marken startet Kampagne mit Patienten im Mittelpunkt 2Marken startet Kampagne mit Patienten im Mittelpunkt 3Marvin Test Solutions' GENASYS Platform Advances Mission-Critical Aerospace Production Test 2Marvin Test Solutions' GENASYS Platform Advances Mission-Critical Aerospace Production Test 3Marvin Test Solutions' GENASYS Platform Advances Mission-Critical Aerospace Production Test 4
... cares for the offspring., Apart from the ones he sucks the ... is now creating a stir in the research world. The ... of organising childcare. In this fish species it is the father ... or more females and then looks after in a brood pouch ...
... 2009 Finishing your plate may soon take on a ... Montral School of Industrial Design, has fashioned edible plates that ... phase of her research, which has resulted in a book ... Edible, The Food as Material. "The idea of using ...
... Ga. University of Georgia scientists looking to understand ... found for the first time in plants an inverse ... has implications for diversity not only in plants, but ... published online in this week,s Proceedings of the ...
Cached Biology News:Brooding fishes take up nutrients from their own children 2Plate-eating good: Université de Montréal professor designs edible tableware 2Study finds new relationship between gene duplication and alternative splicing in plants 2Study finds new relationship between gene duplication and alternative splicing in plants 3
Mouse monoclonal [X223] to Lamin B1 + B2 - Nuclear Envelope Marker ( Abpromise for all tested applications). entrezGeneID: 4001 SwissProtID: P20700...
... (Human Exonic Evidence Based Oligonucleotide) Genome Set ... ) Genome Set contain a collection of ... constitutively expressed exons, allowing interrogation of thousands ... researchers at Stanford University and UCSF, the ...
odz, odd Oz/ten-m homolog 1 [Source:RefSeq_peptide;Acc:NP_055068] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
Rabbit polyclonal to FANCE ( Abpromise for all tested applications). entrezGeneID: 2178 SwissProtID: Q9HB96...
Biology Products: