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Perform Microarray Analysis with Limited Bacterial RNA


Linear RNA amplification technology for bacterial gene expression analyses
As little as 10 ng of mRNA or 100 ng total RNA required for array analysis
Low input requirements enable analysis of mixed host-pathogen samples
Full-length cDNA yields maximized by ArrayScript RT
Highly reproducible and sensitive

Over the past decade, analysis of whole-genome expression profiling with DNA microarrays has revolutionized the way biologists study gene function. RNA amplification has become the preferred method of preparing probes for most microarray platforms. Unfortunately, the benefits of RNA amplification have not been easily accessible to researchers analyzing bacterial gene expression profiles. The absence of poly(A) tails on bacterial mRNA prevents the use of oligo(dT) primed reverse transcription, which is at the heart of most amplification methods. The lack of an appropriate method for bacterial RNA amplification has hampered studies that rely on rare or limited samples, such as environmental collections, small volume cultures, and bacterial RNA isolated from host cells. With over 160 microbial genomes fully sequenced, and many more in progress, an unprecedented opportunity to perform global expression analysis on important pathogens and model organisms is now available.


MessageAmp II-Bacteria Kit
MessageAmp II-Bacteria (patent pending) is based on a linear RNA amplification technology that enables whole
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