Navigation Links
PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

rected values using the custom formula: Correct.Values*!WellPathLength ( Figure 13).


The custom formula for the Dilution column is shown in Figure 14. The term !factor refers to the sample descriptor defined in the template.


The column Final Values contains the corrected absorbance values multiplied by the dilution factor. The custom formula is Correct.Values* Dilution (Figure 15). In the Cuvette Group Table, an analogous column was created to multiply the absorbance values by the dilution factors.


Figure 16 shows the elution profiles from the gel column obtained in the plate and the cuvette. The lower plot (plate values not pathlength corrected), not surprisingly, does not strictly mirror the upper profiles because the volumes in the wells were not uniform. However, when PathCheck was applied to the plate values, the plate profile was essentially superimposable on the cuvette profile.


The above examples illustrate the utility of the PathCheck feature of the SPECTRAmax PLUS. Protein solutions can be read in a microplate and the absorbance values are identical to those obtained by reading the same solutions in a standard 1 cm cuvette. Accurate pipetting is not necessary. An additional benefit of PathCheck is that the linear range is extended above the cuvette linear range (as long as the raw absorbance values in the wells are within the linear range of the instrument).


back to top


'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Advantages of Roche Applied Science amplification products
2. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
3. Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Cell Proliferation and Viability Measurement
6. LabVIEW for Measurement and Data Analysis
7. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
8. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
9. In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate
10. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
11. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
Post Your Comments:
(Date:5/29/2015)... Seoul Semiconductor (CEO: Chung-hoon Lee, ... on May 28th announced that it will increase ... its high efficient and high reliable LED product ... Seoul Semiconductor has developed efficient and reliable LED ... supplied LEDs for headlamps to global auto makers. ...
(Date:5/28/2015)... May 28, 2015 ... announced the addition of Jain PharmaBiotech,s new ... Markets, and Companies" to their offering. ... This report deals with therapeutic drug ... improving patient care by monitoring drug levels ...
(Date:5/28/2015)... May 27, 2015 Research and ... of Jain PharmaBiotech,s new report "Nanobiotechnology ... offering. An increasing use of ... is anticipated. Nanotechnology will be applied at ... formulations for optimal delivery to diagnostic applications ...
(Date:5/28/2015)... May 27, 2015 Research and Markets( ... PharmaBiotech,s new report "Neuroprotection - Drugs, Markets ... This report describes the role of neuroprotection in ... the nervous system as well as in chronic ... the underlying mechanisms of damage to neural tissues ...
Breaking Biology Technology:Seoul Semiconductor Increases the Global Automotive LED Market Share with its High Efficient and Reliable LEDs for Automotive Lightings 2Global Therapeutic Drug Monitoring Market 2015-2024 2Global Nanobiotechnology Market 2015-2024 - Applications, Markets and Companies 2Global Nanobiotechnology Market 2015-2024 - Applications, Markets and Companies 3Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 2Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 3
... Baxter Healthcare Corporation,today announced its V-Link ... first needleless intravenous (IV) connector to,contain an ... in the,United States and Canada. V-Link with ... 99.9 percent or more of specific common ...
... discussed during Omnicell,educational, programs April 15 at ... The Mid-America Club in Chicago, MOUNTAIN ... Inc. (Nasdaq: OMCL ), a leading provider,of ... in,healthcare facilities, and Rice Memorial Hospital today announced ...
... Collins, M.D., Ph.D. a,physician-geneticist and leader of ... of the inaugural Inamori Ethics Prize from ... at Case Western Reserve,University., (Photo: ... Prize honors outstanding international ethical,leaders. It is ...
Cached Biology Technology:Baxter's V-Link Luer-Activated Device With VitalShield Protective Coating Now Available For Hospitals 2Baxter's V-Link Luer-Activated Device With VitalShield Protective Coating Now Available For Hospitals 3Baxter's V-Link Luer-Activated Device With VitalShield Protective Coating Now Available For Hospitals 4Rice Memorial Hospital Implements SinglePointe(TM) Automated Patient-Specific Management Solution From Omnicell 2Rice Memorial Hospital Implements SinglePointe(TM) Automated Patient-Specific Management Solution From Omnicell 3Leading Geneticist Francis Collins Named First Recipient of the Inamori Ethics Prize at Case Western Reserve University 2Leading Geneticist Francis Collins Named First Recipient of the Inamori Ethics Prize at Case Western Reserve University 3Leading Geneticist Francis Collins Named First Recipient of the Inamori Ethics Prize at Case Western Reserve University 4
(Date:5/20/2015)... May 20, 2015 ... has announced the addition of the  ... Iris Recognition Market"  report to their ... research service analyses trends in the ... and commercial sectors, globally. Despite hardware ...
(Date:5/18/2015)... 2015 Fingerprint Cards (FPC) has received ... FPC1155 and FPC1035 from one if its module partners. Deliveries ... Q3 2015 and the sensors will be used by smartphone ... for 2015 hereby amount to 740 MSEK to date. In ... MSEK and a number of smaller orders not separately communicated ...
(Date:5/11/2015)... 11, 2015 Curemark LLC, a privately held ... a new Phase III double blind, randomized, placebo-controlled clinical ... CM-AT, on all children ages 3-8 with Autism. Previously, ... III double blinded clinical trial for CM-AT in children ... the digestive enzyme chymotrypsin. This new trial will help ...
Breaking Biology News(10 mins):Global Iris Recognition Market Report 2015 - 5-Year Opportunity Analysis 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Curemark, LLC, Launches New Phase III Trial in Expanded Population of Children with Autism 2
... Bethesda, MD FASEB MARC (Maximizing Access to ... for the 2012 American Society for Clinical Investigation/Association of ... April 27-29, 2012. These awards are meant to ... scientists into the mainstream of the basic science community ...
... the crypt are supposed to be scary, but new ... and colleagues shows that crypts can be places of ... are small areas of the intestine where new cells ... By focusing on one protein expressed in our intestines ...
... , The decline of Caribbean coral reefs has been linked ... research led by scientists at Scripps Institution of Oceanography at ... news humans are still to blame. The good news ... decline. Employing a novel excavation technique to reconstruct the ...
Cached Biology News:Tales from the crypt lead researchers to cancer discovery 2Declines in Caribbean coral reefs pre-date damage resulting from climate change 2Declines in Caribbean coral reefs pre-date damage resulting from climate change 3
...
...
...
... submicron range are part of your production ... of particle size is important to you. ... detect the component populations in your sample ... Analyzer uses Photon Correlation Spectroscopy (PCS) , ...
Biology Products: