Navigation Links
PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

rected values using the custom formula: Correct.Values*!WellPathLength ( Figure 13).


The custom formula for the Dilution column is shown in Figure 14. The term !factor refers to the sample descriptor defined in the template.


The column Final Values contains the corrected absorbance values multiplied by the dilution factor. The custom formula is Correct.Values* Dilution (Figure 15). In the Cuvette Group Table, an analogous column was created to multiply the absorbance values by the dilution factors.


Figure 16 shows the elution profiles from the gel column obtained in the plate and the cuvette. The lower plot (plate values not pathlength corrected), not surprisingly, does not strictly mirror the upper profiles because the volumes in the wells were not uniform. However, when PathCheck was applied to the plate values, the plate profile was essentially superimposable on the cuvette profile.


The above examples illustrate the utility of the PathCheck feature of the SPECTRAmax PLUS. Protein solutions can be read in a microplate and the absorbance values are identical to those obtained by reading the same solutions in a standard 1 cm cuvette. Accurate pipetting is not necessary. An additional benefit of PathCheck is that the linear range is extended above the cuvette linear range (as long as the raw absorbance values in the wells are within the linear range of the instrument).


back to top


'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Advantages of Roche Applied Science amplification products
2. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
3. Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Cell Proliferation and Viability Measurement
6. LabVIEW for Measurement and Data Analysis
7. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
8. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
9. In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate
10. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
11. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
Post Your Comments:
(Date:8/27/2014)... , Aug. 27, 2014  A novel ... to help physicians better identify early-stage disease, when ... disability, according to a new study in The ... Diagnostics (NYSE: DGX ) and other ... 14-3-3eta protein outperformed conventional antibody-serum testing, including rheumatoid ...
(Date:8/27/2014)... “We are honored to be named to this ... of Radiant Sage. “We are offering truly ground-breaking clinical ... market to a different level. We are enabling ... and effectively manage the massive amounts of medical imaging ... read by trained physicians. Being recognized by technology industry ...
(Date:8/27/2014)... , Aug. 27, 2014 Guggenheim Securities, the ... today announced the hiring of veteran equity analyst Charles ... analyst. Mr. Butler will focus on the biotech/biopharma sector. ... a wealth of experience and a broad network of ... Senior Managing Director and Head of Equities at Guggenheim ...
(Date:8/27/2014)... LONDON , Aug. 27, 2014 ... The Graphene Opportunity - A Rational ... Is Graphene The New Nanotech? A similar ... predictions of applications ranging from microelectronics to water ... today announced the availability of the Graphene Opportunity ...
Breaking Biology Technology:Novel Biomarker Detects Early Rheumatoid Arthritis Better than Conventional Methods Alone, According to Journal of Rheumatology Study 2Novel Biomarker Detects Early Rheumatoid Arthritis Better than Conventional Methods Alone, According to Journal of Rheumatology Study 3Novel Biomarker Detects Early Rheumatoid Arthritis Better than Conventional Methods Alone, According to Journal of Rheumatology Study 4Radiant Sage Named to CIOReview List of 100 Most Promising Technology Companies 2Guggenheim Securities Hires Analyst Charles "Tony" Butler to Expand Healthcare Coverage 2The Graphene Opportunity - A Rational View 2The Graphene Opportunity - A Rational View 3The Graphene Opportunity - A Rational View 4
... QED International Associates, Inc.,administrator for the ... fund portfolios, today announced that effective at ... 2008, Alexion Pharmaceutical, Inc.,(Nasdaq: ALXN ) ... ASPV ) in,the HealthShares(TM) Autoimmune-Inflammation Index. Alpharma, ...
... ARLINGTON, Va., Jan. 4 Faced with ... suppliers are turning their attention to,opportunities in ... navigate these unfamiliar waters, leading research and,advisory ... report,examining the marketing and media preferences of ...
... Md., Jan. 4 Human Genome Sciences,Inc. (Nasdaq: ... 26th,Annual JPMorgan Healthcare Conference will be webcast and may ... ), A member of Human Genome Sciences, ... on Wednesday, January 9, 2008 at 7:30 am Pacific,Time, ...
Cached Biology Technology:QED International Associates Announces Changes to the HealthShares(TM) Autoimmune-Inflammation and Neuroscience Indexes - Correction 2Study Examines Media Preferences of Life Scientists in Applied Markets 2Study Examines Media Preferences of Life Scientists in Applied Markets 3
(Date:8/27/2014)... /PRNewswire-iReach/ -- The IEEE Engineering in Medicine and Biology ... International Conference (EMBC,14), this year being held at the ... Illinois , from Tuesday, August 26 through Saturday, ... industry professionals in science, medicine, and engineering are registered ... This year,s conference theme is ...
(Date:8/27/2014)... as giant, frightening beasts. But every creature is a ... slab containing fossils of 24 very young dinosaurs and ... hatchlings overseen by a caretaker, according to a new ... P. Hedrick and Peter Dodson led the work, collaborating ... where the specimen is held. Hedrick is a doctoral ...
(Date:8/27/2014)... release is available in German . ... is the most common inherited disease affecting the peripheral ... the Max Planck Institute of Experimental Medicine and University ... Schwann cells is impaired in rats with the disease. ... layer known as myelin, which facilitates the rapid transfer ...
Breaking Biology News(10 mins):Global Leaders In Science, Medicine, And Engineering Gather For The 36th Annual International Conference Of The IEEE Engineering In Medicine And Biology Society, August 26-30 In Chicago 2Global Leaders In Science, Medicine, And Engineering Gather For The 36th Annual International Conference Of The IEEE Engineering In Medicine And Biology Society, August 26-30 In Chicago 3Penn paleontologists describe a possible dinosaur nest and young 'babysitter' 2Penn paleontologists describe a possible dinosaur nest and young 'babysitter' 3Potential therapy for incurable Charcot-Marie-Tooth disease 2
... A current controversy raging in evolutionary biology is ... many genes, each of relatively small effect, or just ... published in Molecular Ecology strongly supports the ... from the Department of Biology and the Redpath Museum, ...
... Robot squirrels from the University of California, Davis, ... a research project on the interaction between squirrels and ... how squirrels signal to snakes with heat and tail ... University and UC Davis aim to learn more about ...
... April 3, 2012 A team led by scientists ... prevent infection from widely differing strains of hepatitis C virus ... very high rate of mutation normally helps it to evade ... to sites on the viral envelope that seldom mutate. One ...
Cached Biology News:Robosquirrels versus rattlesnakes 2Robosquirrels versus rattlesnakes 3Scripps Research Institute scientists find promising vaccine targets on hepatitis C virus 2Scripps Research Institute scientists find promising vaccine targets on hepatitis C virus 3Scripps Research Institute scientists find promising vaccine targets on hepatitis C virus 4
...
MSE ANTI CAN. CD3:FITC/RAT ANTI CD4:RPE...
Antibodies were affinity purified using epitopes specific to JLP immobilized on solid support....
This issue concentrated on the purification of proteins for specific purposes (i.e. large-scale purification, purification for therapeutic use, etc.)...
Biology Products: