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PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

e buffer blanks were assigned to wells D6 and D7.

Note: the series feature initially filled all of Row D because SOFTmax PRO by default fills complete rows or columns. Wells D6 through D12 were cleared so that the series would stop at sample #61.

In an analogous manner, the same sample names were assigned to a CuvetteSet section.

Experimental details and customization of the group table
The instrument settings were identical to those used in Example 1 above. Plate pre-read measurements were made in a Costar UV plate. Aliquots of each column fraction and the buffer blank were transferred into their designated wells using disposable transfer pipets, then read in the SPECTRAmax PLUS. As in the example above, the volumes in each well were deliberately varied in order to demonstrate the utility of the PathCheck feature. The column fractions were also read in a quartz cuvette as described above.

The Group Table for the Plate section (after customizing) is shown in Figure 11. Of the seven columns, only three (Sample, Wells and Correct.Values) were created automatically by SOFTmax PRO. (The Correct.Values column was originally entitled Values, but was renamed in this example to distinguish pathlength-corrected values from raw absorbance values. The other four columns were given custom formulas. The Fraction# column was created so that the samples could be given numeric values corresponding to their fraction numbers, then graphed. (In the Sample column, the sample numbers are treated as names and therefore cannot be graphed.)


The custom formula for the Fraction # column ( Figure 12) is Index+20 so that the first entry is 21.


The Raw Values column contains the absorbance values before pathlength correction and were obtained by back-calculation from the cor
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