Navigation Links
PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

each time, to avoid over-writing the pre-read data.

If you use Costar UV plates, you may or may not want to use stored pre-read values, depending on the precision you require. At 280 nm, the average absorbance of Costar wells containing water is approximately .050 + .002 OD. Thus, samples having raw absorbance values of 1 OD would be subject to less than 1% potential error due plate background variation. However, the lower the absorbance in the wells, the greater the maximum potential error due to plate variability.


RESULTS
Example 1: Demonstration of the utility of PathCheck using a protein standard curve
To illustrate the performance of the instrument, a protein standard curve was prepared. Bovine serum albumin (BSA) was dissolved in phosphate-buffered saline (PBS), pH 7.4, filter-sterilized and diluted to 6, 5, 4, 3, 2, 1, 0.5 and 0.25 mg/ mL. Absorbance measurements made in a microplate were compared with measurements made on the same solutions in a 1 cm cuvette.

SOFTmax PRO was launched and a Group entitled Plate Standard was created and given a Standards format in the Template Editor of a Plate section. The standards were assigned to wells as illustrated in Figure 3. Standard 01 was PBS with no added protein (0 mg/mL). An identical template was set up in the Template Editor of a Cuvette Set, except the group was named Cuvette Standard.


The instrument settings and data reduction for the Plate section were set up as shown in Figures 1 and 2 above. The CuvetteSet section was set up for an endpoint read at 280 nm.

A cuvette containing PBS was placed in the cuvette port to serve as the cuvette reference for PathCheck. Pre-read measurements were made with 200 L water in each well of a Costar UV plate, afte
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Advantages of Roche Applied Science amplification products
2. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
3. Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Cell Proliferation and Viability Measurement
6. LabVIEW for Measurement and Data Analysis
7. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
8. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
9. In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate
10. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
11. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
Post Your Comments:
(Date:12/24/2014)... AUSTIN, Texas , Dec. 24, 2014  Vermillion, ... focused on gynecologic disease, announced today that the ... investors including Oracle Investment Management, Jack W. ... directors. Total proceeds were $10.5 million, before offering ...
(Date:12/24/2014)... December 23, 2014 “Preparative & ... HPLC, Flash), Consumables (Columns, Solvents, Buffers, Valves, Guages, ... & Agriculture) - Forecasts to 2019” provides a ... opportunities, current market trends, and strategies impacting the ...
(Date:12/24/2014)... Earlier this year in a June 24 international conference ... Cell Technology Center, LLC ( ASCTC ) focused attention on ... tissue stem cells. His title “Asymmetric Self-Renewal by Distributed ... embodied the essence of his message to congress participants. ...
(Date:12/24/2014)... On Friday, December 19, 2014, President Obama ... Resolution Appropriations Act of 2015, which for the first ... through the Congressionally Directed Medical Research Programs (CDMRP) administered ... (HA), working in conjunction with its allies on Capitol ...
Breaking Biology Technology:Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 2Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 4Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 2Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 4Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2
... July 9 Abt Bio-Pharma Solutions, Inc.,(ABS), ... economic, and,strategic commercialization solutions, is pleased to ... and business strategy, has been promoted to,Executive ... succeeding Susan Windham-Bannister, who has been appointed,President ...
... Provides expertise in high-growth therapeutic areas to pharmaceutical and ... biotechnology companies ... the last year, BEVERLY HILLS, Calif., July 9 Worldwide Clinical,Trials, ... reached a milestone in establishing itself as a premier global,contract research organization ...
... July 9 Cepheid (Nasdaq:,CPHD) today announced ... on Healthcare Associated Infections, Fred Tenover, Ph.D., ... will serve as a liaison,with the Infection ... Infection (HAI) Consortium program., "The objective ...
Cached Biology Technology:Abt Bio-Pharma Solutions, Inc. Names Nandini Hadker as Executive Director, Succeeding Susan Windham-Bannister 2Abt Bio-Pharma Solutions, Inc. Names Nandini Hadker as Executive Director, Succeeding Susan Windham-Bannister 3Worldwide Clinical Trials Emerges as Leading Global Contract Research Organization 2Worldwide Clinical Trials Emerges as Leading Global Contract Research Organization 3Noted Molecular Epidemiologist Joins Cepheid to Head Healthcare Associated Infection Consortium Program 2Noted Molecular Epidemiologist Joins Cepheid to Head Healthcare Associated Infection Consortium Program 3
(Date:1/22/2015)... /PRNewswire/ - Today, FindBiometrics, the leading source of biometrics industry news, ... website design. "When we launched FindBiometrics 12 years ... O,Neill , founder and CEO of FindBiometrics. "Now it,s maturing ... the key players on a very broad scale.  We are ...
(Date:1/22/2015)... 2015  A man-made form of insulin delivered by nasal ... in adults with mild cognitive impairment and Alzheimer,s disease ... at Wake Forest Baptist Medical Center. The ... cognitive impairment (MCI) or mild to moderate Alzheimer,s dementia ...
(Date:1/22/2015)... , January 22, 2015 , ... year  The European Patent Office to present a video ... British nominations to be featured: Christofer Toumazou and ... former finalists and winners of the Award   Starting ...
Breaking Biology News(10 mins):Identity Industry Leading Publication Debuts New Look As Biometrics Hit Mainstream 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 3
... and Zafer Iqbal, research professors at NJIT, were awarded ... arrays of nanoscale electrical probes. Their discovery may ... variation of electrical activity inside biological cells. ... technique that allows for an array of individual, vertically-oriented ...
... DUNES NATIONAL LAKESHORE, Ind. Native bees often small, ... stinging honeybees are quite discriminating about where they ... The study found that, overall, composition of a plant ... a bee community, which may seem counterintuitive at first, ...
... of California, Riverside have made a discovery in the ... drugs and explore novel strategies for treating seizures associated ... Americans. Neurons, the basic building blocks of the ... and chemical signaling. During epileptic seizures, which generally ...
Cached Biology News:Patent for arrays of nanoscale electrical probes awarded to NJIT today 2Picky pollinators: Native bees are selective about where they live and feed 2Picky pollinators: Native bees are selective about where they live and feed 3Picky pollinators: Native bees are selective about where they live and feed 4UC Riverside neuroscientists' discovery could bring relief to epilepsy sufferers 2
Mouse monoclonal [SH-A1] to S100 alpha ( Abpromise for all tested applications). entrezGeneID: 6271 SwissProtID: P23297...
This application changes sensitive keywords in an FCS file to remove patient information. It was contributed by Dr. David Miller....
Rat monoclonal [HK5.3] to B7 RP1 (Biotin) ( Abpromise for all tested applications). entrezGeneID: 23308 SwissProtID: O75144...
Zinc finger MYM-type protein 3 (Zinc finger protein 261) (DXS6673E protein). [Source:Uniprot/SWISSPROT;Acc:Q14202] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
Biology Products: