Navigation Links
PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

t fit properly.
Disposable UV Plate, Corning Costar Corp. (catalog # 3635)
UV-MAX, (Polyfiltronics, Inc., catalog # VO32SCXP8)

3. Pipettor and tips or transfer pipets suitable for use with microplates.

4. Samples (100-300 L each)

5. Quartz cuvette


Set up to make pre-read measurements
Step 1 Launch SOFTmax PRO, then open a Plate section or, if necessary, create a new Plate section.

Step 2 Set up the Instrument Settings dialog box as shown in Figure 1. In the Instrument Settings dialog box, select to perform an endpoint read at 280 nm. Select PathCheck, (Pre-read plate will automatically be checked) and select Cuvette Reference. (Alternatively, you can use the factory-installed Water Constant as a shortcut, though it is potentially less accurate.)


Step 3 Use the Template Editor to create a template showing where standards, blanks, and unknowns will be located on the microplate.

Step 4 Set up the Reduction dialog box as shown in Figure 2. Set the wavelength combination to L1 and the data mode to Absorbance. Make sure that Use pre-read plate and Apply Pathcheck are selected.


METHOD
Making pre-read measurements
The purpose of the pre-read is to subtract the background absorbance of the plate. In contrast, blanks (plate, sample or group) are designated in the template and are subtracted after pathlength correction. In some cases, you may choose to use stored pre-read values (See below).
Step 1 Select a clean microplate and pipet distilled
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Advantages of Roche Applied Science amplification products
2. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
3. Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Cell Proliferation and Viability Measurement
6. LabVIEW for Measurement and Data Analysis
7. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
8. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
9. In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate
10. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
11. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
Post Your Comments:
(Date:12/24/2014)... On Friday, December 19, 2014, President ... and Continuing Resolution Appropriations Act of 2015, which ... eligible to receive funding through the Congressionally Directed ... of Defense (DoD). The Hydrocephalus Association (HA), working ... is celebrating this victory for the over 1 ...
(Date:12/24/2014)... 2014 The report expects global cell ... 2019. It also provides a carefully analyzed data about ... for the market. , Full Copy of Report @ ... global market for cell expansion will keep witnessing growth ... this growth to be driven by rapid technological advancements, ...
(Date:12/24/2014)... -- PharmaBoardroom,s new report, , Healthcare ... for free download , digs deep into this vibrant ... the sector today. One area where ... developing a homegrown pharmaceutical manufacturing base, even if the government,s ... way off. A cursory comparison with neighboring Morocco ...
(Date:12/22/2014)... The Center for Professional Innovation ... training across the life sciences industry, has partnered with ... to provide the organization's members with discounted classroom courses, ... the more than 350 sessions across 80 course titles. ... when registering for a public course at ...
Breaking Biology Technology:Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 2The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 3PharmaBoardroom Releases New Algeria Pharmaceuticals Report 2PharmaBoardroom Releases New Algeria Pharmaceuticals Report 3CfPIE Announces Partnership with the Society for Clinical Data Management 2CfPIE Announces Partnership with the Society for Clinical Data Management 3
... will receive state grants under the Department of ... governor's office said on Monday: , , InvivoSciences ... Developing tests to measure physiological responses of cells. ... planning and grant writing. Makes electronic systems ...
... The state Department of Financial ... copies of annual reports, articles of ... fee through its Web site. , ... program also includes forms for filing ...
... in southeast Wisconsin have agreed to use ... brand for economic development in the area. ... decided on the name, and an accompanying ... The organization is three months old. , ...
Cached Biology Technology:
(Date:12/11/2014)... 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/twhjll/biometrics_market ) has announced the addition of ... 2015-2019"  report to their offering. ... advances in technology. With continuous advances in technology, ... the latest standard that meets the needs and ...
(Date:12/10/2014)... 2014 You,ve been here before: you desperately need ... your password, site key or the answer to your secret ... teacher? Today, Hoyos Labs , a ... will finally put an end to the frustration that comes ... . 1U leverages a user,s smartphone to acquire his or ...
(Date:12/10/2014)... 08, 2014 Research and Markets ... the "Biometrics Market in Japan 2014-2018" ... http://photos.prnewswire.com/prnh/20130307/600769 The integration of ... rural banking and upgradation of the driver,s license ... the market. Besides the aforementioned projects, biometrics is ...
Breaking Biology News(10 mins):Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2
... of researchers led by geneticist Jonathan Sebat, Ph.D., of Cold ... chromosome 16 that substantially increases risk for schizophrenia. ... number variant (CNV). CNVs are areas of the genome where ... CNV is located in a region referred to by scientists ...
... Calif., Oct. 23 GenVault Corporation, the leader in ... identification, today announced that David Wellis, Ph.D., GenVault,s President ... Conference taking place on October 26th and 27th in ... will provide an overview of the company together with ...
... Among the many changes in the ocean is the expansion ... dead zones, which affect the processes by which carbon is ... activities that impact the rate and magnitude of ocean carbon ... known about the metabolism of OMZ microbes and how they ...
Cached Biology News:CSHL-led team discovers rare mutation dramatically increasing schizophrenia risk 2GenVault Corporation to Present at the 2009 BIOCOM Investor Conference 2Model microbial community for studying expanding dead zones characterized 2Model microbial community for studying expanding dead zones characterized 3
Sf21 Insect Cells (Frozen) in Max-XP Medium...
... is used for conventional PCR and thermal ... personal thermal cycler, which features a 96-well ... tubes or plates, programmable time, temperature, and ... modes of temperature control. The system also ...
... ChipWriter Pro system with colony-picking capabilities, 110-120 ... array DNA, proteins, oligonucleotides, carbohydrates, lipids, and ... for microarray applications. This system can also ... as 300 micrometers in diameter and for ...
... Ciphergen's ProteinChip arrays have been developed ... biological samples. A variety of ProteinChip ... proteins according to their unique biochemical ... spots, allowing side-by-side sample comparison, and ...
Biology Products: