Navigation Links
PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)


INTRODUCTION
Protein concentration is commonly estimated by measuring the absorbance at 280 nm and calculating the concentration using the extinction coefficient of the protein. The procedure is feasible because extinction coefficients are typically obtained using standard cuvettes with a fixed 1 cm pathlength. Extinction-based protein assays can also be done in microplates, but they have not been widely-used because of the inconvenience of compensating for variable pathlengths of samples in microplate wells. The introduction of the Molecular Devices SPEC-TRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. The following application note outlines the use of the pathlength correction feature Pathcheck, compares absorbance results obtained in cuvettes and microplate, and gives an example where Pathcheck is applied to column chromatographic fractions and the elution profile displayed.


MATERIALS

For details of the principles of pathlength correction, refer to MAXline application note #25, Optical density measurements automatically normalized to a 1 cm pathlength in the SPECTRAmax PLUS microplate spectrophotometer.

1. SPECTRAmax PLUS microplate spectrophotometer

2. UV-transparent microplates; e.g.:
SPECTRAplate Quartz, Molecular Devices Corp. (catalog # R8024)
SPECTRAplate Quartz half-area microplate, Molecular Devices Corp., (catalog # R8028)
Quartz microtest 8-well strip, Hellma (catalog # 730.010QS); use with a frame for 8-well strips, E & K Scientific Products (catalog # 564101). Note: the 8-well strip frame sold by Evergreen Scientific does no
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Advantages of Roche Applied Science amplification products
2. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
3. Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Cell Proliferation and Viability Measurement
6. LabVIEW for Measurement and Data Analysis
7. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
8. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
9. In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate
10. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
11. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
Post Your Comments:
(Date:9/2/2014)... (PRWEB) September 02, 2014 Myriant ... the signage of a distribution agreement with Azelis ... now distributing Myriant’s bio-succinic acid in the Nordics, ... Ireland, targeting customers in the industrial and base ... powerhouse, Azelis offers a far-reaching chemical portfolio with ...
(Date:9/2/2014)... Opertech Bio, Inc., a company that ... approach to sensory evaluation, today announced the issuance ... Patent and Trademark Office, encompassing high-throughput sensory discrimination ... taste evaluation technology called Microtiter Operant Gustometer or ... for rapid characterization of taste sensory properties,” said ...
(Date:9/2/2014)... , Sept. 2, 2014 Orexigen Therapeutics, Inc. ... U.S. Patent and Trademark Office (PTO) has issued a ... evaluated for weight loss. NB32 is a fixed-dose combination ... U.S. Patent No. 8,815,889 claims methods for treating ... The patent expires in 2024. If NB32 is approved ...
(Date:9/2/2014)... Sept. 2, 2014  Xencor, Inc. (NASDAQ: ... engineered monoclonal antibodies for the treatment of autoimmune ... announced the appointment of Debra Zack , ... appointment of Lloyd Rowland , senior vice ... "Debra,s expertise in biologics clinical development ...
Breaking Biology Technology:Myriant and Azelis Sign European Distribution Agreement for Bio-Succinic Acid 2Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2Orexigen Expands NB32 Intellectual Property Portfolio With New U.S. Patent 2Orexigen Expands NB32 Intellectual Property Portfolio With New U.S. Patent 3Orexigen Expands NB32 Intellectual Property Portfolio With New U.S. Patent 4Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 2Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 3
... Calif., Sept. 16 Sangamo BioSciences, Inc. (Nasdaq: SGMO ... provide an update on the progress of Sangamo,s ZFP Therapeutic(TM) development ... pm ET on Wednesday, September 23, 2009 at the UBS Global ... , , The presentation will be webcast live ...
... HEIDELBERG, Germany, September 16 , ... Drug Targets in Wnt Pathway , ... antagonizes Wnt signalling"** is now available online in,Nature. , ... proteomics,platform was used to identify a small molecule which plays ...
... , BETHESDA, Md., Sept. 16 Micromet, Inc. (Nasdaq: ... the treatment of cancer, inflammation and autoimmune diseases, today announced that its ... Life Sciences Conference on September 22, 2009 in New York City. ... website at www.micromet-inc.com. , , , ...
Cached Biology Technology:Sangamo BioSciences Announces Presentation at the UBS Global Life Sciences Conference 2Cellzome Technology Used to Unravel Novel Regulatory Mechanism in Cancer Signaling Pathway 2Micromet to Present at the UBS Global Life Sciences Conference on September 22, 2009 2Micromet to Present at the UBS Global Life Sciences Conference on September 22, 2009 3
(Date:9/2/2014)... legs and their propensity for eating anything, including each ... now research from North Carolina State University finds that ... the eastern United States. , "The good news is ... of threat to humans," says Dr. Mary Jane Epps, ... of a paper about the research. , The research ...
(Date:9/2/2014)... Tithonian Konservat-Lagersttte of lithographic limestone is well known ... fossils from that area (for example, Archaeopteryx). Now, ... insects in the French equivalent of these outcrops ... the oldest known water treader. , Despite the ... fewer fossils have been obtained from the Late ...
(Date:9/2/2014)... accidentally touch a hot oven, you rapidly pull your ... involved in sensing and responding to such painful stimuli, ... Duke researchers have made a surprising discovery about the ... worms, and have built a structural model of the ... Communications , may help direct new strategies to treat ...
Breaking Biology News(10 mins):Researchers find Asian camel crickets now common in US homes 2Exceptionally well preserved insect fossils from the Rhône Valley 2Surprising new role for calcium in sensing pain 2Surprising new role for calcium in sensing pain 3
... one that doomed most dinosaurs forever alter the varieties of ... doesn,t take a catastrophe to end entire lineages. An analysis ... that vulnerability to extinction runs in evolutionary families, even when ... "Biologists have long suspected that the evolutionary history of species ...
... of novel marine microbes could lead to more efficient ... such as epilepsy, diabetes, flu and other viruses, as ... as agrochemicals. Researchers at Heriot-Watt University and Plymouth ... Ingenza Ltd are searching for new enzymes for use ...
... of Science , conclude that food-web stability is ... intermediate trophic levels. The computations also reveal that small ... the strength of predator-prey links increase the stability of ... consist of interwoven food chains, in which individual animal ...
Cached Biology News:Extinction runs in the family 2Marine microbes creating green waves in industry 2Marine microbes creating green waves in industry 3Scientists find universal rules for food-web stability 2
... CE 4100 high pressure pump with ... with software drive compensation, reducing pulsing to ... selectable in 0.001mL steps. , The pump ... bio-compatible., Pumps may be programmed from their ...
... filter paper sandwiches are composed of a ... of thick filter paper that are preassembled ... sandwich. These 7 x 8.5 cm membrane ... Ready Gel-sized gels and are supplied as ...
... assay kit I provides reagents and a protein ... of reducing agents and detergents. The RC DC ... includes reagents and standards sufficient for 500 standard ... RC reagent II, 250 ml alkaline copper tartrate ...
... is a state-of-the-art separation system using the ... The Eclipse integrates easily with Wyatts multi-angle ... as well as the Optilab DSP. The ... mass distributions of the particles/molecules separated by ...
Biology Products: