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PCR principles

ential Display, Random Amplified Polymorphic DNA (RAPD)].
Introducing molecular tags, such as digoxigenin (DIG) or biotin-labeled dUTP into the PCR product, as it was amplified made PCR an invaluable tool for medical diagnostics. Such labeled PCR products may either be used as hybridization probes or be detected by use of capture probes. For instance, with PCR-generated DIG-labeled hybridization probes, it was possible to detect and quantify minute amounts of a pathogen.
Combining in situ hybridization with PCR (in situ PCR) made possible the localization of single nucleic acid sequences on one chromosome within an eukaryotic organism.

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