Navigation Links
PCR Polymerases Application Profiles

Taq DNA polymerase is still the most commonly used thermostable polymerase for PCR today. However, Taq polymerase has serious limitations like the lack of an error-correcting activity and can only be used for amplification of rather short sequences. To overcome these drawbacks, Roche Applied Science has developed a set of alternative polymerase systems for both, PCR and RT-PCR which are much superior to classic Taq polymerase. To assist you in finding the polymerase system most suitable for your application we have summarized the most important features of each system in a table which you can find on all polymerase product pages. An example with explanations of the listed features is shown below. By clicking on a product name you can directly switch to the respective product page with more detailed information. These links work on every page where you see this type of table.



Length The length of the sequences which can be amplified. Specificity The extent of non specific amplification in particular if difficult templates are to be amplified. The higher the specificity, the lesser by-products are generated. Reproducibility Consistency of results obtained with different enzyme lots. Sensitivity Dependence of amplification efficiency on the amount of template. If the template is limited, an enzyme system with a high sensitivity should be used. Robustness Vulnerability of an enzyme system to contaminating agents or other factors which reduce the amplification efficiency, like strong secondary structures. Accuracy Error rate of an enzyme system as defined by the relative number of misincorporated nucleotides compared to Taq DNA Polymerase. Carryover Prevention Defines whether an enzyme system can be used with dUTP instead of dTTP to apply procedures for PCR carry-over prevention using uracil DNA glycosylase (UNG).
'"/>

Source:


Page: All 1 2

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. A Versatile Power Supply for All Electrophoresis Applications
3. Highest Possible Transformation Efficiencies for High-Throughput Applications
4. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
7. Optimizing Büchi® Rotary Evaporator Applications
8. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
9. Optimizing Büchi® Rotary Evaporator Applications
10. Optimizing Bchi Rotary Evaporator Applications
11. Uses and Applications of X-tremeGENE Q2 Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/11/2017)... LAGUNA HILLS, Calif. , Oct. 11, 2017 /PRNewswire/ ... London (ICR) and University of ... tool to risk-stratify patients with multiple myeloma (MM), in a ... . The University of Leeds is ... Myeloma UK, and ICR will perform the testing services to ...
(Date:10/11/2017)... Bay, Florida (PRWEB) , ... October 11, 2017 ... ... and Drug Administration (FDA) has granted orphan drug designation to SBT-100, its novel ... (sdAb) for the treatment of osteosarcoma. SBT-100 is able to cross the cell ...
(Date:10/10/2017)... ... 10, 2017 , ... For the second time in three ... Mentoring Award. Representatives of the FirstHand program travelled to Washington, D.C. Tuesday, October ... US2020’s mission is to change the trajectory of STEM education in America by ...
(Date:10/10/2017)... 2017 SomaGenics announced the receipt of a ... RealSeq®-SC (Single Cell), expected to be the first commercially ... microRNAs) from single cells using NGS methods. The NIH,s ... accelerate development of approaches to analyze the heterogeneity of ... techniques for measuring levels of mRNAs in individual cells ...
Breaking Biology Technology:
(Date:3/29/2017)... 2017  higi, the health IT company that operates ... America , today announced a Series B investment ... EveryMove. The new investment and acquisition accelerates higi,s strategy ... transform population health activities through the collection and workflow ... higi collects and secures data today on behalf of ...
(Date:3/24/2017)... The Controller General of Immigration from Maldives Mr. Mohamed ... received the prestigious international IAIR Award for the most innovative high security ... ... Maldives Immigration Controller General, ... picture on the right) have received the IAIR award for the "Most ...
(Date:3/23/2017)... Research and Markets has announced the addition of the "Global ... 2025" report to their offering. ... The Global Vehicle Anti-Theft System Market is ... next decade to reach approximately $14.21 billion by 2025. ... all the given segments on global as well as regional levels ...
Breaking Biology News(10 mins):