Navigation Links
PCR Polymerases Application Profiles

Taq DNA polymerase is still the most commonly used thermostable polymerase for PCR today. However, Taq polymerase has serious limitations like the lack of an error-correcting activity and can only be used for amplification of rather short sequences. To overcome these drawbacks, Roche Applied Science has developed a set of alternative polymerase systems for both, PCR and RT-PCR which are much superior to classic Taq polymerase. To assist you in finding the polymerase system most suitable for your application we have summarized the most important features of each system in a table which you can find on all polymerase product pages. An example with explanations of the listed features is shown below. By clicking on a product name you can directly switch to the respective product page with more detailed information. These links work on every page where you see this type of table.



Length The length of the sequences which can be amplified. Specificity The extent of non specific amplification in particular if difficult templates are to be amplified. The higher the specificity, the lesser by-products are generated. Reproducibility Consistency of results obtained with different enzyme lots. Sensitivity Dependence of amplification efficiency on the amount of template. If the template is limited, an enzyme system with a high sensitivity should be used. Robustness Vulnerability of an enzyme system to contaminating agents or other factors which reduce the amplification efficiency, like strong secondary structures. Accuracy Error rate of an enzyme system as defined by the relative number of misincorporated nucleotides compared to Taq DNA Polymerase. Carryover Prevention Defines whether an enzyme system can be used with dUTP instead of dTTP to apply procedures for PCR carry-over prevention using uracil DNA glycosylase (UNG).
'"/>

Source:


Page: All 1 2

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. A Versatile Power Supply for All Electrophoresis Applications
3. Highest Possible Transformation Efficiencies for High-Throughput Applications
4. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
7. Optimizing Büchi® Rotary Evaporator Applications
8. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
9. Optimizing Büchi® Rotary Evaporator Applications
10. Optimizing Bchi Rotary Evaporator Applications
11. Uses and Applications of X-tremeGENE Q2 Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/26/2017)... (PRWEB) , ... April 26, 2017 , ... ... be demonstrating its new Bioflash MailGuardtm mail security screening solution at the National ... The Bioflash MailGuard system provides a fast, highly accurate, easy to use and ...
(Date:4/26/2017)... ... April 26, 2017 , ... As ... conversations among healthcare industry stakeholders, the discussion surrounding the topic will continue at ... 15-18, 2017 in Los Angeles, Calif. Hosted by the Workgroup for Electronic Data ...
(Date:4/25/2017)... (PRWEB) , ... April 25, 2017 , ... As part ... the series will explore the laboratory testing for DIC in order to illuminate this ... disorder which can occur in hospitalized patients resulting in a high degree of morbidity ...
(Date:4/25/2017)... ... April 25, 2017 , ... L3 Clinical Trials, ... the company is now a certified iMedNet eClinical and Electronic Data Capture software ... company’s clinical research team to build, customize and manage clinical trial data capture ...
Breaking Biology Technology:
(Date:4/13/2017)... -- According to a new market research report "Consumer IAM ... and Authorization), Service, Authentication Type, Deployment Mode, Vertical, and Region - Global ... grow from USD 14.30 Billion in 2017 to USD 31.75 Billion by ... ... MarketsandMarkets Logo ...
(Date:4/11/2017)... April 11, 2017 No two people ... at the New York University Tandon School of ... have found that partial similarities between prints are ... in mobile phones and other electronic devices can ... The vulnerability lies in the fact that fingerprint-based ...
(Date:4/5/2017)... April 5, 2017  The Allen Institute for Cell ... Explorer: a one-of-a-kind portal and dynamic digital window into ... data, the first application of deep learning to create ... cell lines and a growing suite of powerful tools. ... these and future publicly available resources created and shared ...
Breaking Biology News(10 mins):