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PCR Polymerases Application Profiles

Taq DNA polymerase is still the most commonly used thermostable polymerase for PCR today. However, Taq polymerase has serious limitations like the lack of an error-correcting activity and can only be used for amplification of rather short sequences. To overcome these drawbacks, Roche Applied Science has developed a set of alternative polymerase systems for both, PCR and RT-PCR which are much superior to classic Taq polymerase. To assist you in finding the polymerase system most suitable for your application we have summarized the most important features of each system in a table which you can find on all polymerase product pages. An example with explanations of the listed features is shown below. By clicking on a product name you can directly switch to the respective product page with more detailed information. These links work on every page where you see this type of table.

Length The length of the sequences which can be amplified. Specificity The extent of non specific amplification in particular if difficult templates are to be amplified. The higher the specificity, the lesser by-products are generated. Reproducibility Consistency of results obtained with different enzyme lots. Sensitivity Dependence of amplification efficiency on the amount of template. If the template is limited, an enzyme system with a high sensitivity should be used. Robustness Vulnerability of an enzyme system to contaminating agents or other factors which reduce the amplification efficiency, like strong secondary structures. Accuracy Error rate of an enzyme system as defined by the relative number of misincorporated nucleotides compared to Taq DNA Polymerase. Carryover Prevention Defines whether an enzyme system can be used with dUTP instead of dTTP to apply procedures for PCR carry-over prevention using uracil DNA glycosylase (UNG).


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