Navigation Links
PCR Polymerases Application Profiles

Taq DNA polymerase is still the most commonly used thermostable polymerase for PCR today. However, Taq polymerase has serious limitations like the lack of an error-correcting activity and can only be used for amplification of rather short sequences. To overcome these drawbacks, Roche Applied Science has developed a set of alternative polymerase systems for both, PCR and RT-PCR which are much superior to classic Taq polymerase. To assist you in finding the polymerase system most suitable for your application we have summarized the most important features of each system in a table which you can find on all polymerase product pages. An example with explanations of the listed features is shown below. By clicking on a product name you can directly switch to the respective product page with more detailed information. These links work on every page where you see this type of table.



Length The length of the sequences which can be amplified. Specificity The extent of non specific amplification in particular if difficult templates are to be amplified. The higher the specificity, the lesser by-products are generated. Reproducibility Consistency of results obtained with different enzyme lots. Sensitivity Dependence of amplification efficiency on the amount of template. If the template is limited, an enzyme system with a high sensitivity should be used. Robustness Vulnerability of an enzyme system to contaminating agents or other factors which reduce the amplification efficiency, like strong secondary structures. Accuracy Error rate of an enzyme system as defined by the relative number of misincorporated nucleotides compared to Taq DNA Polymerase. Carryover Prevention Defines whether an enzyme system can be used with dUTP instead of dTTP to apply procedures for PCR carry-over prevention using uracil DNA glycosylase (UNG).
'"/>

Source:


Page: All 1 2

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. A Versatile Power Supply for All Electrophoresis Applications
3. Highest Possible Transformation Efficiencies for High-Throughput Applications
4. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
7. Optimizing Büchi® Rotary Evaporator Applications
8. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
9. Optimizing Büchi® Rotary Evaporator Applications
10. Optimizing Bchi Rotary Evaporator Applications
11. Uses and Applications of X-tremeGENE Q2 Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/24/2016)... DIEGO , June 24, 2016 ... more sensitively detects cancers susceptible to PARP inhibitors ... circulating tumor cells (CTCs). The new test has ... HRD-targeted therapeutics in multiple cancer types. ... targeting DNA damage response pathways, including PARP, ATM, ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... the release of its second eBook, “Clinical Trials Patient Recruitment and Retention Tips.” ... and retention in this eBook by providing practical tips, tools, and strategies for ...
(Date:6/23/2016)... June 23, 2016 /PRNewswire/ - FACIT has announced ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" ... commercialization of a portfolio of first-in-class WDR5 inhibitors ... such as WDR5 represent an exciting class of ... precision medicine for cancer patients. Substantial advances have ...
(Date:6/23/2016)... ... 23, 2016 , ... Charm Sciences, Inc. is pleased to ... AOAC Research Institute approval 061601. , “This is another AOAC-RI approval of the ... Vice President of Regulatory and Industrial Affairs. “The Peel Plate methods perform comparably ...
Breaking Biology Technology:
(Date:6/22/2016)... , June 22, 2016  The American College of ... Trade Show Executive Magazine as one of the fastest-growing ... May 25-27 at the Bellagio in Las Vegas ... the highest percentage of growth in each of the following ... exhibiting companies and number of attendees. The 2015 ACMG Annual ...
(Date:6/21/2016)... , June 21, 2016 NuData ... the new role of principal product architect and ... the director of customer development. Both will report ... technical officer. The moves reflect NuData,s strategic growth ... response to high customer demand and customer focus ...
(Date:6/15/2016)... June 15, 2016 Transparency ... titled "Gesture Recognition Market by Application Market - Global Industry Analysis ... 2024". According to the report, the  global gesture recognition ... 2015 and is estimated to grow at a ... by 2024.  Increasing application of gesture ...
Breaking Biology News(10 mins):