Navigation Links
PCR Polymerases Application Profiles

Taq DNA polymerase is still the most commonly used thermostable polymerase for PCR today. However, Taq polymerase has serious limitations like the lack of an error-correcting activity and can only be used for amplification of rather short sequences. To overcome these drawbacks, Roche Applied Science has developed a set of alternative polymerase systems for both, PCR and RT-PCR which are much superior to classic Taq polymerase. To assist you in finding the polymerase system most suitable for your application we have summarized the most important features of each system in a table which you can find on all polymerase product pages. An example with explanations of the listed features is shown below. By clicking on a product name you can directly switch to the respective product page with more detailed information. These links work on every page where you see this type of table.



Length The length of the sequences which can be amplified. Specificity The extent of non specific amplification in particular if difficult templates are to be amplified. The higher the specificity, the lesser by-products are generated. Reproducibility Consistency of results obtained with different enzyme lots. Sensitivity Dependence of amplification efficiency on the amount of template. If the template is limited, an enzyme system with a high sensitivity should be used. Robustness Vulnerability of an enzyme system to contaminating agents or other factors which reduce the amplification efficiency, like strong secondary structures. Accuracy Error rate of an enzyme system as defined by the relative number of misincorporated nucleotides compared to Taq DNA Polymerase. Carryover Prevention Defines whether an enzyme system can be used with dUTP instead of dTTP to apply procedures for PCR carry-over prevention using uracil DNA glycosylase (UNG).
'"/>

Source:


Page: All 1 2

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. A Versatile Power Supply for All Electrophoresis Applications
3. Highest Possible Transformation Efficiencies for High-Throughput Applications
4. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
7. Optimizing Büchi® Rotary Evaporator Applications
8. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
9. Optimizing Büchi® Rotary Evaporator Applications
10. Optimizing Bchi Rotary Evaporator Applications
11. Uses and Applications of X-tremeGENE Q2 Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/8/2016)... , December 8, 2016 ... Unternehmen für Molekulargenetik, erweitert seine Palette an anpassbaren ... NGS Custom FH Panels, das ein schnelles und ... ermöglicht. Das Panel bietet eine Erkennung von Einzel-Nukleotid-Variationen ... (CNV) mit einem einzigen kleinen Panel und ermöglicht ...
(Date:12/8/2016)... ... December 08, 2016 , ... ... In response to client demand KbioBox developed a sophisticated “3 click” gene dditing ... are accessible from KBioBox’s new website, https://www.kbiobox.com/ and powered by ...
(Date:12/8/2016)... Iowa (PRWEB) , ... December 08, 2016 , ... This ... asynchronous approvals for biotech crops. The authors focus on the economic effects in countries ... global approval of new biotech crops and the resultant risk of low level presence ...
(Date:12/8/2016)... Fla. , Dec. 8, 2016  HedgePath ... company that discovers, develops and plans to commercialize ... its shares of common stock were approved for ... will begin trading on the OTCQX, effective today, ... qualify for the OTCQX market, companies must meet ...
Breaking Biology Technology:
(Date:12/6/2016)... DALLAS , Dec. 6, 2016 ... criminal justice technology solutions for public safety, investigation, ... (PEP) jointly announced today a five (5) year ... exclusive agreement to expand the rehabilitation and reentry ... PEP History Established in 2004, the Prison ...
(Date:12/2/2016)... 2016   SoftServe , a global digital ... an electrocardiogram (ECG) biosensor analysis system for continuous ... asset. The smart system ensures device-to-device communication between ... and mobile devices to easily ,recognize, and monitor ... vehicle technology advances, so too must the security ...
(Date:11/29/2016)... , Nov. 29, 2016 BioDirection, a privately ... products for the objective detection of concussion and other ... has successfully completed a meeting with the U.S. Food ... blood test Pre-Submission Package. During the meeting company representatives ... as a precursor to commencement of a planned pilot ...
Breaking Biology News(10 mins):