PCR Performance Comparisons Between pfuturbo and Taq DNA,,,Polymerases ( In this update additional PCR pe...)

PCR Performance Comparisons Between pfuturbo and Taq DNA,,,Polymerases

In this update, additional PCR performance comparisons are presented. PfuTurbo DNA polymerase and Taq DNA polymerase are compared with respect to PCR product yield and amplification of complex targets.

PCR Performance Comparisons

Figure 1 shows results from amplifying a series of primer-template systems using PfuTurbo DNA polymerase or Taq DNA polymerase. The a1-antitrypsin gene was amplified from human genomic DNA, using a series of 3 staggered primers to determine the maximum target length that can be amplified by each enzyme (lanes 1-4, PfuTurbo DNA polymerase; lanes 7-10, Taq DNA polymerase). Both enzymes successfully amplified the 0.9- and 2.0-kb targets in high yield. PfuTurbo DNA polymerase amplified the longer 3.9- and 5.2-kb targets in significantly higher yield than Taq DNA polymerase, and little smearing was observed. In contrast, significant smearing was evident in amplifications of 3.9-kb targets with Taq DNA polymerase.

Superior performance of PfuTurbo DNA polymerase was also observed when additional primer-template systems were tested. For example, PfuTurbo DNA polymerase successfully amplified an 8-kb target from rodent genomic DNA (lane 5) and a 14-kb target from lambda phage DNA (lane 6). In contrast, Taq DNA polymerase had difficulty amplifying these relatively long amplicons (lanes 11 and 12).

Fidelity Comparisons

The error rates of a number of DNA polymerases have been measured previously in a PCR-based forward mutation assay, which utilizes the lacIOlacZa target gene.³ In these studies, Pfu DNA polymerase exhibited an average error rate that was two-fold lower than that of Vent and Deep Vent DNA polymerases,³ three- to six-fold lower than those of DNA polymerase mixtures,^{3
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( In this update additional PCR pe...)PCR Performance Comparisons Between pfuturbo and Taq DNA,,,Polymerases

PCR Performance Comparisons

Fidelity Comparisons