Navigation Links
PCR Performance Comparisons Between pfuturbo and Taq DNA,,,Polymerases


PCR Performance Comparisons Between PfuTurbo and Taq DNA Polymerases

pfuturbo DNA polymerase is the superior choice for robust high-fidelity PCR amplification

Holly Hogrefe Frances Bai Janice Cline
Stratagene

In this update, we compare the PCR performances of pfuturbo DNA polymerase *, and Taq DNA polymerase. PCR product yields were higher (>3-kb targets) or comparable (0- to 2-kb targets) with PfuTurbo DNA polymerase using one-minute-per-kb extension times. The error rate of PfuTurbo DNA polymerase, like that of Pfu DNA polymerase, was significantly lower than the error rate of Taq DNA polymerase. Thus, PfuTurbo DNA polymerase is superior for both routine and high-performance, high-fidelity PCR applications.

Stratagene recently introduced PfuTurbo DNA polymerase for robust, high-fidelity PCR amplification.1 PfuTurbo DNA polymerase is a special formulation of cloned Pfu DNA polymerase and a newly discovered PCR-enhancing factor that dramatically improves PCR product yields without altering DNA replication fidelity. This novel PCR-enhancing factor was isolated from Pyrococcus sp. and lacks both DNA polymerase and exonuclease activities.**

PfuTurbo DNA polymerase has been shown to be ideally suited for amplifying genomic DNA targets up to 10 kb, and cloned targets up to 15 kb, with the highest replication fidelity possible. Initial studies revealed that PCR product yields are dramatically higher with PfuTurbo DNA polymerase than with Pfu DNA polymerase (alone) or other commercially available proofreading PCR enzymes.1 Moreover, high-fidelity amplification is achieved using one-minute-per-kb extension times with vector targets up to 10 kb and genomic targets up to 6 kb, rather than the two-minute-per-kb extension times recommended for Pfu DNA polymerase.2'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
4. Performance Comparisons of Commercial RT-PCR Systems
5. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
6. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
7. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
8. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
9. Highly Sensitive microRNA Array Performance
10. High Throughput RNA Isolation for High Performance Microarray Analysis
11. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
Post Your Comments:
(Date:4/28/2015)... , and ANN ARBOR, Mich. ... small cohort published OncoTargets and Therapy today ... better than Foundation Medicine,s FoundationOne test across a number ... clinically actionable biomarker targets. The purpose of ... commercially available NGS platforms for profiling cancer patients compared ...
(Date:4/28/2015)... DE (PRWEB) April 28, 2015 The ... organizations that work together to lower barriers to innovation ... Ontologies Mapping project. , The Ontologies Mapping project ... establish best practices for ontology management in life sciences ... a set of standardized guidelines, tools and services to ...
(Date:4/28/2015)... (PRWEB) April 28, 2015 More than ... psychosis at some point over the course of their ... symptoms that worsen as the disease progresses. ReachMD ... announces the launch of a series dedicated to PD ... in PD, movement disorders, and psychiatric conditions. , The ...
(Date:4/28/2015)... According to a 2013 report from the American ... from burn injuries per year. Despite several advances in ... scars with subsequent contractures remains a major problem in ... no adequate treatment. Contractures are a permanent tightening of ... permanent deformity. , Synedgen has developed a new ...
Breaking Biology Technology:Paradigm Cancer Diagnostic Test (PCDx) Performed Better Than FoundationOne In Side by Side Clinical Study 2Paradigm Cancer Diagnostic Test (PCDx) Performed Better Than FoundationOne In Side by Side Clinical Study 3Pistoia Alliance Announces Start of Ontologies Mapping Project 2ReachMD Launches Series Focusing on Parkinson’s Disease Psychosis 2Synedgen Burn Treatment Results Presented at Premier Wound Conference 2Synedgen Burn Treatment Results Presented at Premier Wound Conference 3
... , LEXINGTON, Ky. , ... based in Lexington, KY announced today that its ... created and characterized the world,s first rat model of severe ... complete lack of the major components of the immune system. ...
... 6 Omnio Healer AB, a pioneer company,developing novel ... has entered into a partnership agreement with Eden,Biodesign. Under ... production services relating to Omnio Healer,s lead,recombinant protein candidate. ... therapeutic concept for wound healing and,anti-infection, Omnio Healer is ...
... WALTHAM, Mass., Aug. 6 Repligen Corporation (Nasdaq: ... 2010, ended June 30, 2009. Total revenue for the quarter ... quarter of fiscal year 2009. The prior year results were ... Company for royalties on the U.S. sales of Orencia(R) prior to ...
Cached Biology Technology:Transposagen Creates a Rat Model of the Human Immune System 2Transposagen Creates a Rat Model of the Human Immune System 3Omnio Healer Selects Eden Biodesign for cGMP Production of its Candidate Wound Healing and Anti-Infection Treatments 2Omnio Healer Selects Eden Biodesign for cGMP Production of its Candidate Wound Healing and Anti-Infection Treatments 3Repligen Reports First Quarter Fiscal Year 2010 Financial Results 2Repligen Reports First Quarter Fiscal Year 2010 Financial Results 3Repligen Reports First Quarter Fiscal Year 2010 Financial Results 4Repligen Reports First Quarter Fiscal Year 2010 Financial Results 5Repligen Reports First Quarter Fiscal Year 2010 Financial Results 6Repligen Reports First Quarter Fiscal Year 2010 Financial Results 7
(Date:4/13/2015)... 2015 According to a ... Market Forecast & Opportunities, 2020", the global biometrics market ... 14% till 2020. The biometrics market is garnering ... and review of biometric management systems. The constant ... with greater efficiency, are resulting in increasing installation ...
(Date:4/6/2015)... 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ... focused on the growing mobile commerce market, announced ... for MINIATURE, MULTI-PURPOSE ANTENNA METHOD AND SYSTEM ... NXT-ID introduces a new revolutionary method and system ... use of miniature antenna modules for multiple purposes ...
(Date:4/1/2015)... Apr. 1, 2015  NXT-ID, Inc. (NASDAQ: NXTD ... focused on the growing mobile commerce market, announces the ... wallets is underway to early access pre-order customers. ... usage at retail outlets including Walmart, Target, AT&T, Dunkin, ... Wocket was accepted at all outlets and very easy ...
Breaking Biology News(10 mins):Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2NXT-ID Patents Miniature, Low Power Wireless Magnetic Stripe 2NXT-ID Patents Miniature, Low Power Wireless Magnetic Stripe 3NXT-ID Patents Miniature, Low Power Wireless Magnetic Stripe 4NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 2NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 3NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 4
... from the University of California at San Francisco (UCSF) ... dedicated to the brain that the controversy is over: ... dead. , The cerebral cortex is a sheet of ... that process particular aspects of sensation, movement and cognition. ...
... of genes has shed light on the role of gene ... in Proceedings of the National Academy of Sciences USA, was ... , Dr Robert Beiko, Professor Mark Ragan and Mr ... in an effort to map how genes are shared between ...
... used by the U.S. military to track vehicle convoys in ... little-known world of pygmy elephants in Borneo. , ... elephant's being captured and outfitted with a collar that can ... the first time, the public can track the movements of ...
Cached Biology News:MIT researcher presents new view of how the cortex forms 2Lateral thinking produces first map of gene transmission 2WWF peeks into mysterious life of Borneo's pygmy elephants 2
Human Ig Lambda-chain, clone ICO-16, Monoclonal Antibody...
Rabbit polyclonal to Warfarin ( Abpromise for all tested applications). Antigen: Chemical / Small Molecule conjugated to KLH...
WNV Core Antibody...
... Rabbit polyclonal to FANCM ( Abpromise ... Antigen: Synthetic peptide derived from ... Human FANCM. (Note: the amino acid ... available as ab27787 .) ...
Biology Products: