Navigation Links
PCR Performance Comparisons Between pfuturbo and Taq DNA,,,Polymerases


PCR Performance Comparisons Between PfuTurbo and Taq DNA Polymerases

pfuturbo DNA polymerase is the superior choice for robust high-fidelity PCR amplification

Holly Hogrefe Frances Bai Janice Cline
Stratagene

In this update, we compare the PCR performances of pfuturbo DNA polymerase *, and Taq DNA polymerase. PCR product yields were higher (>3-kb targets) or comparable (0- to 2-kb targets) with PfuTurbo DNA polymerase using one-minute-per-kb extension times. The error rate of PfuTurbo DNA polymerase, like that of Pfu DNA polymerase, was significantly lower than the error rate of Taq DNA polymerase. Thus, PfuTurbo DNA polymerase is superior for both routine and high-performance, high-fidelity PCR applications.

Stratagene recently introduced PfuTurbo DNA polymerase for robust, high-fidelity PCR amplification.1 PfuTurbo DNA polymerase is a special formulation of cloned Pfu DNA polymerase and a newly discovered PCR-enhancing factor that dramatically improves PCR product yields without altering DNA replication fidelity. This novel PCR-enhancing factor was isolated from Pyrococcus sp. and lacks both DNA polymerase and exonuclease activities.**

PfuTurbo DNA polymerase has been shown to be ideally suited for amplifying genomic DNA targets up to 10 kb, and cloned targets up to 15 kb, with the highest replication fidelity possible. Initial studies revealed that PCR product yields are dramatically higher with PfuTurbo DNA polymerase than with Pfu DNA polymerase (alone) or other commercially available proofreading PCR enzymes.1 Moreover, high-fidelity amplification is achieved using one-minute-per-kb extension times with vector targets up to 10 kb and genomic targets up to 6 kb, rather than the two-minute-per-kb extension times recommended for Pfu DNA polymerase.2'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
4. Performance Comparisons of Commercial RT-PCR Systems
5. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
6. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
7. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
8. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
9. Highly Sensitive microRNA Array Performance
10. High Throughput RNA Isolation for High Performance Microarray Analysis
11. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
Post Your Comments:
(Date:10/19/2014)... 2014 The report "Unmanned Ground ... Security), Technology, Payload, Type & by Geography - ... global unmanned ground vehicle market into various sub ... market sizes. The report also identifies the factors ... it along with the adoption trends. , The ...
(Date:10/18/2014)... The report "1,6-Hexanediol Market by Application ... 2019", analyzes the global market on the basis ... industry details such as the key drivers, current ... , Browse 81 Market Data Tables and 29 ... on “1,6-Hexanediol Market by Application and by Geography ...
(Date:10/18/2014)... 2014 The Asian Electronic Medical Record (EMR) ... with analysis and forecast of revenue. The electronic medical record ... $2,328.0 million by 2018, at a CAGR of 9.4% from ... Asian Electronic Medical Record (EMR) market, to get an idea ... of the segmentation of the market in the same region, ...
(Date:10/18/2014)... The “Global Diagnostic Electrocardiograph (ECG) Market ... (Hospitals, Home/Ambulatory, Research Center, Physician Office, Nursing Homes), ... to 2020” provides a detailed overview of the ... and strategies impacting the global diagnostic ECG market ... revenue and share analysis. , The global diagnostic ...
Breaking Biology Technology:Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 2Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 3Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 51,6-Hexanediol Market Projected to Reach $916.1 Million at a CAGR of 8.3% by 2019 – Report by MarketsandMarkets 21,6-Hexanediol Market Projected to Reach $916.1 Million at a CAGR of 8.3% by 2019 – Report by MarketsandMarkets 31,6-Hexanediol Market Projected to Reach $916.1 Million at a CAGR of 8.3% by 2019 – Report by MarketsandMarkets 41,6-Hexanediol Market Projected to Reach $916.1 Million at a CAGR of 8.3% by 2019 – Report by MarketsandMarkets 5The Asian Electronic Medical Record (EMR) market is estimated to grow to $2,328.0 million by 2018 - New Report by MicroMarket Monitor 2The Asian Electronic Medical Record (EMR) market is estimated to grow to $2,328.0 million by 2018 - New Report by MicroMarket Monitor 3The Asian Electronic Medical Record (EMR) market is estimated to grow to $2,328.0 million by 2018 - New Report by MicroMarket Monitor 4The Asian Electronic Medical Record (EMR) market is estimated to grow to $2,328.0 million by 2018 - New Report by MicroMarket Monitor 5The Electrocardiograph (ECG) Market Is Expected to Reach $5,435 Million By 2020 - New Research Report by MarketsandMarkets 2The Electrocardiograph (ECG) Market Is Expected to Reach $5,435 Million By 2020 - New Research Report by MarketsandMarkets 3The Electrocardiograph (ECG) Market Is Expected to Reach $5,435 Million By 2020 - New Research Report by MarketsandMarkets 4The Electrocardiograph (ECG) Market Is Expected to Reach $5,435 Million By 2020 - New Research Report by MarketsandMarkets 5
... Neb., Nov. 6 Transgenomic (OTC,Bulletin Board: TBIO) today announced ... the Rodman & Renshaw 10th Annual,Healthcare Conference to be held ... York City. Mr. Tuttle and Debra Schneider, Chief,Financial Officer, will ... Event: Rodman & Renshaw 10th Annual ...
... researchers are showing that Microsoft SQL ... up to 1 petabyte., REDMOND, Wash., Nov. ... its data management and business,intelligence platform, Microsoft SQL ... software to scale new heights in data warehousing,and ...
... AHCC for cancer patients undergoing chemotherapy, PURCHASE, ... in,the Journal of the Society for Integrative Oncology ... researchers at the University of Texas M.D.,Anderson Cancer ... Up,Chemical Company in Sapporo, Japan has demonstrated that ...
Cached Biology Technology:Transgenomic to Present at Rodman & Renshaw 10th Annual Healthcare Conference 2Could Database Software Help Cure Alzheimer's and Save the Earth? 2Could Database Software Help Cure Alzheimer's and Save the Earth? 3Study From the University of Texas M.D. Anderson Cancer Center Demonstrates That AHCC(R) is Safe in Combination With Most Chemotherapeutic Agents 2Study From the University of Texas M.D. Anderson Cancer Center Demonstrates That AHCC(R) is Safe in Combination With Most Chemotherapeutic Agents 3
(Date:10/15/2014)... Gauß-Allianz has admitted Johannes Gutenberg University Mainz (JGU) ... science hub continues to maintain a significant nationwide ... , "The acceptance of Johannes Gutenberg University Mainz ... a milestone in the national and international competition ... state of Rhineland-Palatinate can be proud that Mainz ...
(Date:10/15/2014)... , Oct. 15, 2014   Neurotechnology ... today announced the availability of the VeriLook ... Surveillance 3.0 provides real-time biometric face identification using ... digital surveillance cameras. The new version not only ... differentiates people from objects while they are moving ...
(Date:10/14/2014)... One of the planet,s leading questions is how to ... increasingly variable climate. The Food and Agriculture Organization of ... 70% over the next 40 years to feed a ... of the necessary rise in food production. Plants—grains, ... by supporting livestock. Current research must tap into ...
Breaking Biology News(10 mins):Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 2Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 3VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 2VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 3Building a bridge from basic botany to applied agriculture 2Building a bridge from basic botany to applied agriculture 3
... research team coordinated at the IRCM in Montral found ... Dr. Tarik Mry, the IRCM,s President and Scientific Director, ... "Achilles, heel" and could be targeted to develop a ... current treatments such as chemotherapy and radiation therapy. The ...
... common element but is found in old paints (including ... water, and the atmosphere from lead-containing vehicular fuels, even ... also causes seemingly trivial symptoms such as headaches. However, ... to the organs, the kidneys in particular, and the ...
... prostate cancer is the most common cancer in elderly ... aged between 35 and 50. Scientists at the European ... with several other research teams in Germany*, have discovered ... different mechanism from that which causes the disease at ...
Cached Biology News:Researchers discover 'Achilles' heel' for lymphoid leukemia 2Is lead poisoning behind some juvenile crime? 21 disease, 2 mechanisms 2
...
... Detection Kit is intended for quantitative detection ... peptides, which can result from the gradual, ... aspartic acid residues during storage or handling. ... the monitoring of charge differences for detection, ...
...
...
Biology Products: