( PCR Performance Comparisons Between P...)PCR Performance Comparisons Between pfuturbo and Taq DNA,,,Polymerases

pfuturbo DNA polymerase is the superior choice for robust high-fidelity PCR amplification

Holly Hogrefe Frances Bai Janice Cline
Stratagene

In this update, we compare the PCR performances of pfuturbo DNA polymerase *^, and Taq DNA polymerase. PCR product yields were higher (>3-kb targets) or comparable (0- to 2-kb targets) with PfuTurbo DNA polymerase using one-minute-per-kb extension times. The error rate of PfuTurbo DNA polymerase, like that of Pfu DNA polymerase, was significantly lower than the error rate of Taq DNA polymerase. Thus, PfuTurbo DNA polymerase is superior for both routine and high-performance, high-fidelity PCR applications.

Stratagene recently introduced PfuTurbo DNA polymerase for robust, high-fidelity PCR amplification.¹ PfuTurbo DNA polymerase is a special formulation of cloned Pfu DNA polymerase and a newly discovered PCR-enhancing factor that dramatically improves PCR product yields without altering DNA replication fidelity. This novel PCR-enhancing factor was isolated from Pyrococcus sp. and lacks both DNA polymerase and exonuclease activities.**

PfuTurbo DNA polymerase has been shown to be ideally suited for amplifying genomic DNA targets up to 10 kb, and cloned targets up to 15 kb, with the highest replication fidelity possible. Initial studies revealed that PCR product yields are dramatically higher with PfuTurbo DNA polymerase than with Pfu DNA polymerase (alone) or other commercially available proofreading PCR enzymes.¹ Moreover, high-fidelity amplification is achieved using one-minute-per-kb extension times with vector targets up to 10 kb and genomic targets up to 6 kb, rather than the two-minute-per-kb extension times recommended for Pfu DNA polymerase.<sup>2'"/>

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