Navigation Links
PCR Master

plification of single-copy genes from eukaryotic genomes
  • Labeling of PCR products with modified nucleotides (DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
  • Prevention of carry-over contamination, by incorporation of dUTP and degradation of contaminating DNA with Uracil-DNA Glycosylase
  • Cycle sequencing
  • Operating parameters
    • pH optimum: approx. 9 (adjusted at 20C)
    • Temperature optimum (for elongation): approx. 75C
      Note: The DNA polymerase has a half life at 95C of approx. 40 min
    • Divalent ion requirement: Mg2+ (standard concentration, 1.5 mM)
    • dNTP requirement: approx. 200 M for each dNTP
    Effects of additives Tween 20 (0.51.0%) detergent has been used to enhance the efficiency of Taq DNA Polymerase in certain PCRs. Other additives reported to have enhancing effects include DMSO, gelatine, glycerol, betaine, spermidine, T4 gene 32 protein, BSA, and ammonium sulfate. Key advantages
    • High PCR yield, because it is stable during prolonged, repetitive high temperature incubations
    • Enhances specificity of amplification, because it is highly processive and free of exonuclease and nicking activities
    • Increases sensitivity, because it produces good yields even when template amounts are limiting
    • Best results, because it has proved superior to competitors Taq DNA Polymerase preparations (see "Not all Taq DNA polymerase preparations are the same'"/>


    Source:


    Page: All 1 2 3

    Related biology technology :

    1. TripleMaster PCR System
    2. TripleMaster PCR System
    3. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
    4. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
    5. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
    6. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
    7. HotMasterAn innovative Hot Start/Cold Stop technology for better PCR* results
    8. Enhancing PCR* Efficiency with MasterTaq
    9. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
    10. The use of TaqMaster PCR Enhancer
    11. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: PCR Master

    (Date:3/26/2015)...  BioNano Genomics, Inc., the leader in genome mapping, ... chief commercial officer. Salyer has more than 20 years ... life sciences. At BioNano, Salyer will oversee the commercialization ... to assemble a comprehensive view of complex genomes to ... institutions located in the United States ...
    (Date:3/26/2015)... 26, 2015 Neogen Corporation (NASDAQ: NEOG ... third quarter of fiscal 2015, which ended Feb. 28, ... year,s $6,575,000. Earnings per share in the current quarter ... year-to-date net income increased 17% over prior year to ... $0.56 per share, for the same period a year ...
    (Date:3/26/2015)... ReliantHeart, Inc., an innovative supplier of advanced mechanical ... transit-time flow measurement solutions, are collaborating on a miniature ... the HeartAssist5® Left Ventricular Assist Device (LVAD). ... and peace of mind. , The Transonic flow ... 1/6th of the power required by earlier flow board ...
    (Date:3/26/2015)... Ale Gicqueau, Founder and President of Clinovo, will ... Align Technology, on “ Conducting Postmarket Registries - Rationale ... Conference and Exhibition on April 25th through April 28th ... poster presentation, Ale Gicqueau and Victor Chen will discuss ... the requirements and resources to conduct the registry, as ...
    Breaking Biology Technology:BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 2BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 3Neogen reports 13% increase in net income 2Neogen reports 13% increase in net income 3Neogen reports 13% increase in net income 4Neogen reports 13% increase in net income 5Neogen reports 13% increase in net income 6Neogen reports 13% increase in net income 7Neogen reports 13% increase in net income 8LVAD patients to have greater mobility and peace of mind through Transonic and ReliantHeart’s True Flow collaboration for the HeartAssist5® 2LVAD patients to have greater mobility and peace of mind through Transonic and ReliantHeart’s True Flow collaboration for the HeartAssist5® 3Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 2Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 3
    ... Wis. Information-technology businesses are taking apart their ... Fusion 2005 CEO-CIO Symposium on Wednesday just around ... Wisconsin offered their opinions on how the state and ... the year progresses. , Restructuring and rebuilding ,According ...
    ... education products in Wisconsin Rapids, has sold Generation21 Learning ... ,Generation21 lost $4.6 million before taxes on $2.6 million ... looked for strategic alternatives over the past few years ... option. , ,The company will record a $600,000 gain ...
    ... Wis. There may no yellow tape or chalk ... of computer crimes if they want remedies, said a lawyer ... on Wednesday. , ,Daniel Vaccaro, a partner with Michael ... been compromised by Internet fraudsters or disgruntled employees can benefit ...
    Cached Biology Technology:Restructuring is a watchword for IT in 2005 2Restructuring is a watchword for IT in 2005 3After a successful attack on your computer systems, then what? 2After a successful attack on your computer systems, then what? 3
    (Date:3/19/2015)... 2015  NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" ... growing mobile commerce market, announces its biometric payment technology, the ... aired this week on Washington DC,s ... a segment "The Next Great Thing", host Laura Evans ... pay, and ,a really big breakthrough in mobile payment.,  ...
    (Date:3/16/2015)... 2015 Between 16 and ... groundbreaking innovations in biometric identification at the CeBIT ...      (Photo: http://photos.prnewswire.com/prnh/20150316/735333 ) ... well at the forefront: the company,s all new ... Germany . BiometricGate is the ...
    (Date:3/11/2015)... and Markets ( http://www.researchandmarkets.com/research/3p32qm/access_control ) has announced ... Product, Application & By Geography - Global Forecast & ... This report predicts that the access control ... with an estimated CAGR of 10.6%. The ... as contact cards & readers, contactless cards & readers, ...
    Breaking Biology News(10 mins):NXT-ID's Wocket Smart Wallet Featured on Fox News Segment, "The Next Great Thing" 2NXT-ID's Wocket Smart Wallet Featured on Fox News Segment, "The Next Great Thing" 3DERMALOG Presents its Latest Biometric Innovations at the Hannover CeBIT Exhibition, 16 - 20 March 2015 2DERMALOG Presents its Latest Biometric Innovations at the Hannover CeBIT Exhibition, 16 - 20 March 2015 3Global Access Control (Cards & Readers, Biometrics, Controllers, Management Software) Market 2015-2020 - Analysis of the $10 Billion Market 2
    ... of its kind, researchers at Queens College and Mount ... status (SES) and maternal gestational diabetes together may cause ... (ADHD) in six year olds. The data are published ... Pediatrics and Adolescent Medicine , one of the JAMA/Archives ...
    ... Group, Inc. (Pinksheets: EVRM) would like to issue the ... foremost, I want to thank our supporters; it has ... Evermedia Group, advancing the interests of wholly owned subsidiaries: ... have been fortunate to work with many outstanding professionals ...
    ... Chimpanzees, orangutans, gorillas and bonobos make more sophisticated decisions ... success, based on what they know and the likelihood to ... Daniel Haun, published on December 21 in the online journal ... human decision-making as well. The authors of the ...
    Cached Biology News:Gestational diabetes and low socioeconomic status raise risk of ADHD in children 2The Evermedia Group 2011 Year in Review 2The Evermedia Group 2011 Year in Review 3The Evermedia Group 2011 Year in Review 4
    Normal human AB serum, pooled...
    sterile filtered, off the clot, all male donors, Total Protein: 4.5 to 8.5 g/dl...
    XBP1 Antibody...
    Recombinant Mouse MMP-9, CF...
    Biology Products: