for routine amplification of up to 3 kb genomic DNA targets Cat. No. 1 636 103 (100 reactions) Description The PCR Master Kit contains 10 vials of 2x concentrated PCR reaction mix (Taq DNA Polymerase, reaction buffer with MgCl₂, stabilizing detergent, dNTPs) plus water PCR grade (for dilution), sufficient for 100 PCR reactions. Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM. It is now supplied as a recombinant enzyme from E. coli. The enzyme is highly purified and is free of nonspecific endo- or exonucleases. Taq DNA Polymerase consists of a single polypeptide chain with a molecular weight of approximately 95 kD. It is a highly processive 5'3' DNA polymerase which lacks 3'5' exonuclease activity.

Note: For additional convenience and reliable PCR with minimum reagent preparation, we offer the PCR Master [Cat. No. 1636103, 2x conc. PCR reaction mix (Taq DNA Polymerase, reaction buffer with MgCl2, stabilising detergent, dNTPs)] plus water PCR grade for dilution. Further the PCR Core Kit (Cat. No. 1578553), containing Taq DNA Polymerase, dNTP stock solution, PCR reaction buffer with and without MgCl₂ and a MgCl₂ stock solution. Application Amplifies genomic targets up to 3 kb with good yield and specificity. Capable of amplifying lambda DNA up to 10 kb. The enzyme is ideal for:

• Routine am
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