Navigation Links
PCR Master

for routine amplification of up to 3 kb genomic DNA targets Cat. No. 1 636 103 (100 reactions) Description The PCR Master Kit contains 10 vials of 2x concentrated PCR reaction mix (Taq DNA Polymerase, reaction buffer with MgCl2, stabilizing detergent, dNTPs) plus water PCR grade (for dilution), sufficient for 100 PCR reactions. Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM. It is now supplied as a recombinant enzyme from E. coli. The enzyme is highly purified and is free of nonspecific endo- or exonucleases. Taq DNA Polymerase consists of a single polypeptide chain with a molecular weight of approximately 95 kD. It is a highly processive 5'3' DNA polymerase which lacks 3'5' exonuclease activity.

Note: For additional convenience and reliable PCR with minimum reagent preparation, we offer the PCR Master [Cat. No. 1636103, 2x conc. PCR reaction mix (Taq DNA Polymerase, reaction buffer with MgCl2, stabilising detergent, dNTPs)] plus water PCR grade for dilution. Further the PCR Core Kit (Cat. No. 1578553), containing Taq DNA Polymerase, dNTP stock solution, PCR reaction buffer with and without MgCl2 and a MgCl2 stock solution. Application Amplifies genomic targets up to 3 kb with good yield and specificity. Capable of amplifying lambda DNA up to 10 kb. The enzyme is ideal for:
  • Routine am plification of single-copy genes from eukaryotic genomes
  • Labeling of PCR products with modified nucleotides (DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
  • Prevention of carry-over contamination, by incorporation of dUTP and degradation of contaminating DNA with Uracil-DNA Glycosylase
  • Cycle sequencing
Operating parameters
  • pH optimum: approx. 9 (adjusted at 20C)
  • Temperature optimum (for elongation): approx. 75C
    Note: The DNA polymerase has a half life at 95C of approx. 40 min
  • Divalent ion requirement: Mg2+ (standard concentration, 1.5 mM)
  • dNTP requirement: approx. 200 M for each dNTP
Effects of additives Tween 20 (0.51.0%) detergent has been used to enhance the efficiency of Taq DNA Polymerase in certain PCRs. Other additives reported to have enhancing effects include DMSO, gelatine, glycerol, betaine, spermidine, T4 gene 32 protein, BSA, and ammonium sulfate. Key advantages
  • High PCR yield, because it is stable during prolonged, repetitive high temperature incubations
  • Enhances specificity of amplification, because it is highly processive and free of exonuclease and nicking activities
  • Increases sensitivity, because it produces good yields even when template amounts are limiting
  • Best results, because it has proved superior to competitors Taq DNA Polymerase preparations (see "Not all Taq DNA polymerase preparations are the same")


'"/>


Source:


Page: All 1 2 3

Related biology technology :

1. TripleMaster PCR System
2. TripleMaster PCR System
3. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
4. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
5. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
6. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
7. HotMasterAn innovative Hot Start/Cold Stop technology for better PCR* results
8. Enhancing PCR* Efficiency with MasterTaq
9. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
10. The use of TaqMaster PCR Enhancer
11. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/4/2016)... ... 04, 2016 , ... PBI-Gordon Corporation is pleased to announce Doug Obermann has ... his career at PBI-Gordon in February 1988, after finishing his masters in agronomy from ... from customer service to national product manager, to helping develop, name and launch many ...
(Date:5/3/2016)... ... May 03, 2016 , ... Morf Media ... compliance training platform on mobile devices, today released a new interactive Food ... Medical Devices. The course is essential for owners or operators of places of ...
(Date:5/3/2016)... CO (PRWEB) , ... May 03, 2016 , ... ... , announced the addition of Dr. Nancy Gillett to its Board of Directors. ... position, she served as Corporate Executive Vice President and Chief Scientific Officer. A ...
(Date:5/3/2016)... 2016 - And Other Rising ... of Those Competitor Biologics  - Biosimilar Drug ... Prospects ,  Who are the most important ... are their sales potentials? Discover, in our updated survey, ... opportunities and revenue forecasting. Visiongain,s ...
Breaking Biology Technology:
(Date:4/13/2016)... CHICAGO , April 13, 2016  IMPOWER physicians ... are setting a new clinical standard in telehealth ... By leveraging the higi platform, IMPOWER patients can ... weight, pulse and body mass index, and, when they ... quick and convenient visit to a local retail location ...
(Date:3/22/2016)... March 22, 2016 ... Sensors Market for Consumer Industry by Type (Image, ... Application (Communication & IT, Entertainment, Home Appliances, ... Forecast to 2022", published by MarketsandMarkets, the ... to reach USD 26.76 Billion by 2022, ...
(Date:3/15/2016)... New York , March 15, 2016 ... new market report published by Transparency Market Research "Digital Door ... Trends and Forecast 2015 - 2023," the global digital door ... US$ 731.9 Mn in 2014 and is forecast to grow ... 2023. Growth of micro, small and medium enterprises (MSMEs) across ...
Breaking Biology News(10 mins):