Navigation Links
PCR Grade Water

Cat. No. 3 315 932, 25 ml (25 vials of 1 ml)
Cat. No. 3 315 959, 25 ml (1 vials of 25 ml)
Cat. No. 3 315 843, 100 ml (4 vials of 25 ml)) Description Specially purified, double distilled, deionized, auto-claved water Free of detectable endonucleases, ribonucleases and nicking activity Routinely function checked in PCR pH at 20C: 8.3 Application This product is especially tested and qualified for use in all PCR or RT-PCR reactions, in conjunction with conventional thermocyclers or with the LightCycler Instrument available from Roche Applied Science. All LightCycler Kits available from Roche Applied Sci-ence and some of the Amplification Products available from Roche Applied Science contain this Water, PCR Grade. Storage and Stability Store a working solution for a short period of time
(e.g. 1 week) at 2 - 8 C Store stock solutions for a longer period of time
at 15 to 25 C Quality Control Test buffer: 10 mM Tris/HCl, pH 7.5 (37C), 10 mM MgCl 2 , 1 mM DTE Absence of endonucleases: 1 g DNA is incubated with various amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade shows no detectable degradation of the DNA. Absence of endonucleases: 1 g DNA, cleaved by Eco RI and Hind III (MWM III) is incubated with various amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade sh ows no detectable degradation of the DNA fragments. Absence of nicking activity: 1 g supercoiled pBR322 DNA is incubated with vari-ous amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade shows no detectable alteration or relaxation of the supercoiled DNA. Absence of ribunocleases: 5 g of MS2 RNA are incubated with various amouts of Water, PCR Grade in 50 l test buffer at 37C for 1 h. Up to 20 l Water, PCR Grade shows no detectable RNAse activity. Amplification function test: Each lot of Water, PCR Grade is function tested in PCR. Routinely, PCR is performed using DNA for an 0.5 kb fragment. A clear defined band from 0.01 ng DNA should be clearly visible. Notice to purchase

This product is optimized for use in the Polymerase Chain Reaction (PCR) covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche). No license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. PCR Grade Deoxynucleotides
2. Enjoy a Greater Sense of Security with PCR-Grade Water
3. PCR Grade Deoxynucleotides Additional power to perfect your PCR experiments
4. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
5. PCR-Grade Nucleotides Larger Pack Sizes Now Available
6. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
7. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
8. Which Water to Use?
9. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
10. Analysis of Phenolic Estrogen Mimics in River Water by Negative Ion Electrospray LC/MS/MS
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/10/2017)... (PRWEB) , ... October 10, 2017 , ... ... innovation and business process optimization firm for the life sciences and healthcare industries, ... conference in San Francisco. , The presentation, “Automating GxP Validation for Agile ...
(Date:10/9/2017)... ... 2017 , ... At its national board meeting in North Carolina, ARCS® ... Departments of Physics and Astronomy, has been selected for membership in ARCS Alumni ... the 2015 Breakthrough Prize in Fundamental physics for the discovery of the accelerating expansion ...
(Date:10/7/2017)... ... October 06, 2017 , ... ... technologies, launched its ProxiMeta™ Hi-C metagenome deconvolution product, featuring the first commercially ... cloud-based bioinformatics software to perform Hi-C metagenome deconvolution using their own facilities, ...
(Date:10/5/2017)... YORBA LINDA, CA (PRWEB) , ... October 05, 2017 , ... ... tech innovators, engineers, and scientists from around the world, is giving back to cancer ... shirt sold in October. , Now through October 31, shoppers can use promo ...
Breaking Biology Technology:
(Date:6/14/2017)... IBM ) is introducing several innovative partner startups at VivaTech ... startups and global businesses, taking place in Paris ... will showcase the solutions they have built with IBM Watson ... France is one of the most dynamic ... in the number of startups created between 2012 and 2015*, ...
(Date:4/24/2017)... Janice Kephart , former 9/11 ... Partners, LLP (IdSP) , today issues the following ... March 6, 2017 Executive Order: Protecting the ... be instilled with greater confidence, enabling the reactivation ... applications are suspended by until at least July ...
(Date:4/13/2017)... 2017 According to a new market research report ... Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, Vertical, and Region ... expected to grow from USD 14.30 Billion in 2017 to USD 31.75 ... ... MarketsandMarkets Logo ...
Breaking Biology News(10 mins):