Navigation Links
PCR Grade Water

Cat. No. 3 315 932, 25 ml (25 vials of 1 ml)
Cat. No. 3 315 959, 25 ml (1 vials of 25 ml)
Cat. No. 3 315 843, 100 ml (4 vials of 25 ml)) Description Specially purified, double distilled, deionized, auto-claved water Free of detectable endonucleases, ribonucleases and nicking activity Routinely function checked in PCR pH at 20C: 8.3 Application This product is especially tested and qualified for use in all PCR or RT-PCR reactions, in conjunction with conventional thermocyclers or with the LightCycler Instrument available from Roche Applied Science. All LightCycler Kits available from Roche Applied Sci-ence and some of the Amplification Products available from Roche Applied Science contain this Water, PCR Grade. Storage and Stability Store a working solution for a short period of time
(e.g. 1 week) at 2 - 8 C Store stock solutions for a longer period of time
at 15 to 25 C Quality Control Test buffer: 10 mM Tris/HCl, pH 7.5 (37C), 10 mM MgCl 2 , 1 mM DTE Absence of endonucleases: 1 g DNA is incubated with various amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade shows no detectable degradation of the DNA. Absence of endonucleases: 1 g DNA, cleaved by Eco RI and Hind III (MWM III) is incubated with various amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade sh ows no detectable degradation of the DNA fragments. Absence of nicking activity: 1 g supercoiled pBR322 DNA is incubated with vari-ous amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade shows no detectable alteration or relaxation of the supercoiled DNA. Absence of ribunocleases: 5 g of MS2 RNA are incubated with various amouts of Water, PCR Grade in 50 l test buffer at 37C for 1 h. Up to 20 l Water, PCR Grade shows no detectable RNAse activity. Amplification function test: Each lot of Water, PCR Grade is function tested in PCR. Routinely, PCR is performed using DNA for an 0.5 kb fragment. A clear defined band from 0.01 ng DNA should be clearly visible. Notice to purchase

This product is optimized for use in the Polymerase Chain Reaction (PCR) covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche). No license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. PCR Grade Deoxynucleotides
2. Enjoy a Greater Sense of Security with PCR-Grade Water
3. PCR Grade Deoxynucleotides Additional power to perfect your PCR experiments
4. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
5. PCR-Grade Nucleotides Larger Pack Sizes Now Available
6. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
7. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
8. Which Water to Use?
9. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
10. Analysis of Phenolic Estrogen Mimics in River Water by Negative Ion Electrospray LC/MS/MS
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... ... June 23, 2016 , ... Mosio, ... second eBook, “Clinical Trials Patient Recruitment and Retention Tips.” Partnering with experienced clinical ... eBook by providing practical tips, tools, and strategies for clinical researchers. , “The ...
(Date:6/23/2016)... /PRNewswire/ - FACIT has announced the creation of ... company, Propellon Therapeutics Inc. ("Propellon" or "the Company"), ... portfolio of first-in-class WDR5 inhibitors for the treatment ... represent an exciting class of therapies, possessing the ... cancer patients. Substantial advances have been achieved with ...
(Date:6/23/2016)... ... June 23, 2016 , ... Charm ... Mold) microbial test has received AOAC Research Institute approval 061601. , “This is ... last year,” stated Bob Salter, Vice President of Regulatory and Industrial Affairs. “The ...
(Date:6/23/2016)... SAN FRANCISCO , June 23, 2016   ... it has secured $1 million in debt financing from ... to ramp up automation and to advance its drug ... for its new facility. "SVB has been ... goes beyond the services a traditional bank would provide," ...
Breaking Biology Technology:
(Date:6/2/2016)... , June 2, 2016 Perimeter ... Platforms, Unmanned Systems, Physical Infrastructure, Support & Other Service  ... visiongain offers comprehensive analysis of the global ... market will generate revenues of $17.98 billion in 2016. ... DVTEL Inc, a leader in software and hardware technologies ...
(Date:5/12/2016)... WearablesResearch.com , a brand of Troubadour ... from the Q1 wave of its quarterly wearables survey. ... receptivity to a program where they would receive discounts ... company. "We were surprised to see that ... LaColla , CEO of Troubadour Research, "primarily because there ...
(Date:4/26/2016)... LONDON , April 26, 2016 ... a product subsidiary of Infosys (NYSE: ... to integrate the Onegini mobile security platform with ... http://photos.prnewswire.com/prnh/20151104/283829LOGO ) The integration will ... to access and transact across channels. Using this ...
Breaking Biology News(10 mins):