Roche Applied Science now offers new larger pack sizes
of PCR-Grade Nucleotides. These larger pack sizes
combine the best available quality with attractive pricing.
Single dNTPs are now available in 500-mol pack sizes.
The Deoxynucleoside Triphosphate Set, containing all four dNTPs (dATP, dCTP, dGTP, and dTTP) in separate
vials, is now available in a 4 x 125-mol pack size. This
larger pack size is sufficient for 6,250 100-l PCRs at a
final dNTP concentration of 0.2 mM. The 500-mol pack
size of single dNTPs provides 25,000 reactions.
In addition, dideoxynucleoside triphosphates (ddNTPs)
are now offered in a sequencing-grade form the highest
possible quality available. Sequencing-Grade ddNTPs
are supplied in a set (ddATP, ddCTP, ddGTP, and ddTTP)
as sodium salt solutions at pH 8.3.
High-Quality PCR-Grade Nucleotides
PCR-Grade and Sequencing-Grade Nucleotides from
Roche Applied Science are specially manufactured and
purified to the highest possible chemical purity. PCRGrade
Nucleotides contain at least 99% of the relevant
deoxynucleoside triphosphate (dNTP) and less than
0.9% deoxynucleoside diphosphate (dNDP). They are
provided as clear, colorless solutions in water at a
concentration of 100 mM (pH 8.3). Due to the manufacturing
process, PCR-Grade Nucleotides are free of
dNTPs with modified bases, as well as tetra- and
pyrophosphate contamination. Tetraphosphate can disintegrate
and form pyrophosphate, which is a known
inhibitor of amplification reactions. Therefore, the
nucleotide purity is critical to the success of your amplification
reactions. PCR-Grade Nucleotides are stable for
30 months after production when stored at 20 C.
For your convenience, PCR-Grade Nucleotides are available
as nucleotide sets (all four nucleotides in separate
vials), as single nucleotides, as kits (dNTPs, buffer, and
polymerase in separate vials), ready-to-use mixes (premixed
solutions of all four nucleotides), and ready-touse
mixes that contain polymerase, nucleotides, and
buffer (premixed 2x solution).
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with PCR-Grade Water