Navigation Links
PCR Core KitPLUS

Kit for PCR and PCR carry-over prevention Cat. No. 1 585 541 (1 Kit = 50 reactions) Description The PCR Core KitPLUS supplies all basic reagents to perform a polymerase chain reaction with the exception of template DNA and appropriate primers. The kit contains:
  • Heat-labile UNG
  • Taq DNA Polymerase
  • PCR Nucleotide MixPLUS
  • PCR reaction buffer with MgCl2 (10x conc.)
  • PCR reaction buffer without MgCl2 (10x conc.)
  • MgCl2 stock solution
Application Complete reagent set for prevention of carry-over contamination in PCR with Taq DNA Polymerase. Operating parameters
  • Substrate: Single- or double-stranded DNA containing deoxyuracil (DNA synthesized in the presence of dUTP).
  • Incubation conditions for cleavage of uracil-glycosidic bonds: 20C, 110 min.
  • Thermal stability (pH 8.3): half-life at 40C, 2 min; half-life at 45C, 0.5 min.
  • MgCl2 concentration: 2.5 mM.
  • dNTP concentration: 200 M dNTPs; 600 M dUTP.
  • Conditions for inactivation of enzyme: 95C, 2 min.

Notes: UNG (2 units) will degrade up to 106 uracil-containing DNA molecules in 10 min. In contrast to the enzyme from E. coli, heat-labile UNG will not modify dU-containing PCR products for at least several hours at 4C. Therefore, it is not necessary to freeze dU-containing PCR products immediately after amplificatio n, or to hold the reaction mixture at 72C until it is used. However, note that after prolonged storage (>8 h) residual enzyme activity may lead to degradation of PCR product. For long term storage, PCR product should therefore be frozen at 20C.

Key advantages
  • Convenient, includes all necessary reagents (except template) to perform PCR and carry-over prevention.
  • Reliable removal of carry-over contamination in PCR, because the kit contains heat-labile UNG.
  • Maximum flexibility, contains two reaction buffers
    (one with MgCl2 and one without MgCl2).
  • Easy-to-use, because all included reagents are ready-to-use solutions.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. PCR Core KitPLUS
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... , June 27, 2016  Liquid Biotech ... announced the funding of a Sponsored Research Agreement ... circulating tumor cells (CTCs) from cancer patients.  The ... in CTC levels correlate with clinical outcomes in ... These data will then be employed to support ...
(Date:6/24/2016)... ... ... While the majority of commercial spectrophotometers and fluorometers use the z-dimension of 8.5 ... end machines that use the more unconventional z-dimension of 20mm. Z-dimension or ... the cuvette holder. , FireflySci has developed several Agilent flow cell product lines ...
(Date:6/23/2016)...   Boston Biomedical , an industry leader ... target cancer stemness pathways, announced that its lead ... Designation from the U.S. Food and Drug Administration ... gastroesophageal junction (GEJ) cancer. Napabucasin is an orally ... stemness pathways by targeting STAT3, and is currently ...
(Date:6/23/2016)... ... June 23, 2016 , ... Charm ... Mold) microbial test has received AOAC Research Institute approval 061601. , “This is ... last year,” stated Bob Salter, Vice President of Regulatory and Industrial Affairs. “The ...
Breaking Biology Technology:
(Date:6/9/2016)... leader in attendance control systems is proud to announce the introduction of fingerprint attendance ... the right employees are actually signing in, and to even control the opening of ... ... ... Photo - ...
(Date:6/3/2016)... , June 3, 2016 ... von Nepal hat ... Lieferung hochsicherer geprägter Kennzeichen, einschließlich Personalisierung, Registrierung ... in der Produktion und Implementierung von Identitätsmanagementlösungen. ... Ausschreibung im Januar teilgenommen, aber Decatur wurde ...
(Date:6/2/2016)... -- Perimeter Surveillance & Detection Systems, Biometrics ... Support & Other Service  The latest report ... analysis of the global Border Security market . ... $17.98 billion in 2016. Now: In November ... software and hardware technologies for advanced video surveillance. ...
Breaking Biology News(10 mins):