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PCR Core KitPLUS

Kit for PCR and PCR carry-over prevention Cat. No. 1 585 541 (1 Kit = 50 reactions) Description The PCR Core KitPLUS supplies all basic reagents to perform a polymerase chain reaction with the exception of template DNA and appropriate primers. The kit contains:
  • Heat-labile UNG
  • Taq DNA Polymerase
  • PCR Nucleotide MixPLUS
  • PCR reaction buffer with MgCl2 (10x conc.)
  • PCR reaction buffer without MgCl2 (10x conc.)
  • MgCl2 stock solution
Application Complete reagent set for prevention of carry-over contamination in PCR with Taq DNA Polymerase. Operating parameters
  • Substrate: Single- or double-stranded DNA containing deoxyuracil (DNA synthesized in the presence of dUTP).
  • Incubation conditions for cleavage of uracil-glycosidic bonds: 20C, 110 min.
  • Thermal stability (pH 8.3): half-life at 40C, 2 min; half-life at 45C, 0.5 min.
  • MgCl2 concentration: 2.5 mM.
  • dNTP concentration: 200 M dNTPs; 600 M dUTP.
  • Conditions for inactivation of enzyme: 95C, 2 min.

Notes: UNG (2 units) will degrade up to 106 uracil-containing DNA molecules in 10 min. In contrast to the enzyme from E. coli, heat-labile UNG will not modify dU-containing PCR products for at least several hours at 4C. Therefore, it is not necessary to freeze dU-containing PCR products immediately after amplificatio
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