Navigation Links
PCR Core KitPLUS

Kit for PCR and PCR carry-over prevention Cat. No. 1 585 541 (1 Kit = 50 reactions) Description The PCR Core KitPLUS supplies all basic reagents to perform a polymerase chain reaction with the exception of template DNA and appropriate primers. The kit contains:
  • Heat-labile UNG
  • Taq DNA Polymerase
  • PCR Nucleotide MixPLUS
  • PCR reaction buffer with MgCl2 (10x conc.)
  • PCR reaction buffer without MgCl2 (10x conc.)
  • MgCl2 stock solution
Application Complete reagent set for prevention of carry-over contamination in PCR with Taq DNA Polymerase. Operating parameters
  • Substrate: Single- or double-stranded DNA containing deoxyuracil (DNA synthesized in the presence of dUTP).
  • Incubation conditions for cleavage of uracil-glycosidic bonds: 20C, 110 min.
  • Thermal stability (pH 8.3): half-life at 40C, 2 min; half-life at 45C, 0.5 min.
  • MgCl2 concentration: 2.5 mM.
  • dNTP concentration: 200 M dNTPs; 600 M dUTP.
  • Conditions for inactivation of enzyme: 95C, 2 min.

Notes: UNG (2 units) will degrade up to 106 uracil-containing DNA molecules in 10 min. In contrast to the enzyme from E. coli, heat-labile UNG will not modify dU-containing PCR products for at least several hours at 4C. Therefore, it is not necessary to freeze dU-containing PCR products immediately after amplificatio
'"/>

Source:


Page: All 1 2

Related biology technology :

1. PCR Core KitPLUS
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: PCR Core KitPLUS

(Date:8/22/2014)... “Our nation's healthcare system is ... disgrace. Furthermore, every governmental and professional entity that ... state of unorganized dysfunction shares in that enormous ... serve as a device to use in an ... that have accumulated through the years leading to ...
(Date:8/21/2014)... international research team including DESY scientists has observed tiny ... team reports in the journal Science that ... inside the nanodroplets. It is the first time that ... larger samples of what is known as superfluid helium, ... our best expectations," says Andrey Vilesov of the University ...
(Date:8/21/2014)... COLD SPRING, N.Y. , Aug. 21, 2014 ... today the launch of their Nutraceutical "TELO-20 for ... first and only Telomere-lengthening supplement in the world ... the ends of every chromosome in the body. ... the world,s leading experts in Telomere Science and ...
(Date:8/21/2014)... 21, 2014 OTC Markets Group ... a biotechnology company, on its approval to list ... on OTCQX®, the best marketplace for established U.S. ... Logo - http://photos.prnewswire.com/prnh/20110118/MM31963LOGO ... execution of its growth strategy and achievement of ...
Breaking Biology Technology:5 Great Mistakes in Healthcare and How to Fix Them by Dr. Ira Williams 2Scientists observe quantum vortices in cold helium droplets 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 3OTC Markets Group Congratulates Immune Pharmaceuticals on NASDAQ Listing 2
... Inc.,(Nasdaq: VRML ), a molecular diagnostics company, ... its board of directors. Mr. Hamilton is,the former ... Inc., a,specialty pharmaceutical company., "John brings more ... strategic and audit matters to Vermillion. His solid ...
... Interim Leadership Responsibility, PRINCETON, N.J., April 10 ... the discovery and development of novel,small molecule therapeutics, ... as President and Chief Executive Officer and a ... pursue other interests. His resignation was,effective April 9, ...
... SEATTLE, April 10 Amnis Corporation announced today ... analysis software.,IDEAS version 3.0 incorporates greatly expanded analytical ... IDEAS 3.0 provides the user with dozens ... to strengthen morphological,data analysis. The new software also ...
Cached Biology Technology:Vermillion Announces Appointment of John Hamilton to Board of Directors 2Leslie J. Browne Resigns as President and Chief Executive Officer of Pharmacopeia, Inc. 2Leslie J. Browne Resigns as President and Chief Executive Officer of Pharmacopeia, Inc. 3Leslie J. Browne Resigns as President and Chief Executive Officer of Pharmacopeia, Inc. 4Amnis Announces Release of Powerful New Image Data Analysis Software 2
(Date:8/22/2014)... antioxidants and can reduce free radical damage. ... oxidative stress? Jianbo Zhao and co-workers from ... University, China discovered that green tea polyphenol ... apoptosis, indicating green tea polyphenols play a ... oxidative stress. The relevant study has been ...
(Date:8/21/2014)... smog-producing toxins in past decade, GTA still violates ... that while the Greater Toronto Area (GTA) has ... to smog, the city continues to violate the ... can cause or aggravate health problems such as ... a set of complex photochemical reactions involving volatile ...
(Date:8/21/2014)... dandruff, eczema and other itchy, flaky maladies in humans ... Hawaiian coral reefs and the extreme environments of arctic ... the scientific journal PLOS Pathogens considers the ... genus Malassezia in light of new insights ... world. , University of Hawai,i at Mānoa scientist Anthony ...
Breaking Biology News(10 mins):Despite a significant reduction in smog-producing toxins, the Greater Toronto Area still violates Canada's standards for ozone air pollution 2From dandruff to deep sea vents, an ecologically hyper-diverse fungus 2
... and Amsterdam, The Netherlands, January 14, 2008 A ... E genotypes has been developed by researchers from the ... Southern Medical University, Guangzhou, China. This assay combines PCR ... genotyping of the human population, which in turn will ...
... have a skull that functioned like a fish-eating crocodile, despite ... claws, perhaps used as grappling hooks to lift fish from ... Bristol, UK, used computer modelling techniques more commonly used ... to show that while Baryonyx was eating, its skull ...
... 20,000 people found seven new genes that influence blood ... confirmed 11 other genes previously thought to influence ... from the University of Michigan School of Public Health ... influence lipid levels, and secondly, to see if those ...
Cached Biology News:New method developed to identify genetic determinants of Alzheimer's disease 2Unusual fish-eating dinosaur had crocodile-like skull 2Study locates cholesterol genes; finds surprises about good, bad cholesterol 2
geminin, DNA replication inhibitor...
Normal human serum, single donor...
One-step, microplate or cuvet, colorimetric, linear detection range 0.1 mg/dL to 100 mg/dL. Procedure: 20 min....
... Certified USDA Research Registered OLAW Assured AAALAC ... AALAS Certified Technicians Documentation ... Upon Request Focus CRP is ... your custom immunology service needs. We ...
Biology Products: