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P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor

ll weight. To maintain consistency, the media, nutrients, inoculum, base, and control set-up was the same as the first run, except for the DO cascade as listed below.


Dissolved Oxygen (DO) Control
Cascade..Agitation and Oxygen

The cascade first increased agitation and then added oxygen gas as needed to maintain the DO at setpoint.

Figure 4 shows that the DO declined steadily towards the 30% setpoint during the first 10 hours, while agitation changed as required, to maintain the DO setpoint. After reaching 1,200 rpm at ~24 hours, further oxygen demand went uncompensated, causing dissolved oxygen to drop below the setpoint.






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