Navigation Links
P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor

h as substrate concentration and metabolite build-up can also be limiting, but these are often more controllable than inherent physical limitations of the fermentor.


Conclusion
Pichia pastoris growth in the BioFlo 110 was successful. Culture density of 91.0 g/L DCW was achieved, and when oxygen supplementation was added (see Appendix, page 5), cell density reached 177.4 g/L DCW.

Temperature control was excellent using unchilled (55F) tap water as the coolant. Nevertheless, fermentors with large-area stainless-steel heat exchangers, such as the New Brunswick Scientifics BioFlo 3000, have an advantage in temperature control at high cell density compared to systems with immersed coils (like the BioFlo 110 used here); or when compared to systems that rely on waterjackets made of glass. Glass has poorer thermal conductivity than stainless steel, but glass jackets have larger surface areas than immersed coils. Both immersed coils and glass jackets can work well, but the advantage of a fermentor like the BioFlo 3000 with a large stainless steel heat exchanger becomes significant at higher cell densities or with higher coolant temperatures.

Overall, our protocol and the BioFlo 110 performed extremely well. The BioFlo 110 Advanced Fermentation Kit is a suitable instrument for culturing Pichia pastoris . Furthermore, we expect that similar results can be achieved when using BioFlo 110 fermentation vessels in other sizes (1.3, 3.0 or 14 L liters), as well as when using a water-jacket configuration.


APPENDIX:
Effect of Gas Mix Controller and Oxygen Supplementation

A second continuous-batch was performed, this time adding the BioFlo 110 Gas Mix Controller, , M1273- 3104, to demonstrate the impact of oxygen supplementation on final dry ce
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. Fermentation Cell Culture: University Style
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
8. Outstanding transfection rates using the Multiporator
9. Outstanding transfection rates using the Multiporator
10. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
11. Normalizing cDNA libraries using the EppendorfThermomixer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: pastoris Fermentation using BioFlo Benchtop Fermentor

(Date:8/27/2015)... Aug. 27, 2015  Neogen Corporation (NASDAQ: NEOG ... of United Kingdom -based Lab M ... media and diagnostic systems. Lab M ... leading provider of microbial testing and diagnostic products for ... The company currently sells into more than 70 countries ...
(Date:8/27/2015)... PARK, Calif. , Aug. 27, 2015  Asterias ... in the emerging field of regenerative medicine, today announced ... investor conferences in the month of September.  ... will present at Rodman & Renshaw,s 17th Annual Global ... ET/7:25am PT at the St. Regis Hotel in ...
(Date:8/27/2015)... -- GlassesOff (OTCBB: GLSO), a visual neuroscience technology ... vision system, announced today a partnership with eight-time All-Star, ... to develop a new mobile app designed for professional ... on-court performance. Vision is the inception of every ... ball or blocking a pass. The critical impact vision speed ...
(Date:8/27/2015)... Aug. 27, 2015 In response to ... UPS and The US Postal Service to no ... as ,select agents, by the CDC, Marken reaffirmed ... these sensitive shipments. Marken uses its ... trained and certified personnel to ensure the safe ...
Breaking Biology Technology:Neogen acquires U.K.-based Lab M Holdings 2Asterias Biotherapeutics to Present at Upcoming Investor Conferences 2Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 2Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 3Marken Ensures Safe Transportation Of Select Agents And Toxins 2
... May 9 ATS Medical, Inc. (Nasdaq:,ATSI), manufacturer ... that it will participate at the Rodman & ... Meridien Beach Plaza,Hotel in Monte Carlo on Tuesday, ... ATS Medical, will present at 9:20 a.m. local ...
... N.J., May 9 Celator Pharmaceuticals today,announced that ... Jackson joined Celator in October 2007 as Head ... Scott as the new CEO. He brings more ... of specialty,pharmaceutical experience primarily in oncology," said Dr. ...
... China, May 9 /Xinhua-PRNewswire-FirstCall/ -- China-Biotics,Inc. (OTC Bulletin ... specializing in the manufacture, research, development,marketing and distribution ... work for its new plant at Qing Pu, ... 150 MT capacity is the cornerstone of our,growth ...
Cached Biology Technology:ATS Medical to Participate at the Rodman & Renshaw 5th Annual Global Healthcare Conference 2Celator(R) Pharmaceuticals Announces Appointment of Scott Jackson as CEO 2China-Biotics, Inc. Completes Foundation for Its New Plant 2
(Date:8/28/2015)... 28, 2015 According to a ... by Assessment Type (Pen & Paper Based, Hosted, Biometrics), ... Learning, Academic Research), Vertical and Region - Global Forecast ... Market to grow from USD 2.4 Billion in 2015 ... Annual Growth Rate (CAGR) of 25.6% from 2015 to ...
(Date:8/26/2015)... PUNE, India , August 26, 2015 ... (Two, Three, Four, and Five Factor), Application (Travel & ... and Geography - Global Forecast to 2020", published by ... 9.60 Billion by 2020, growing at a CAGR of ... 71 Tables and 82 F igures spread through ...
(Date:8/25/2015)... and BELLEVUE, Wash. , Aug. ... advanced robotic systems, announced today it will unveil its ... vehicle (UGV), at the National Tactical Officers Association Conference ... The Guardian S is the first-ever commercially available ... years of research and in-field trials and is protected ...
Breaking Biology News(10 mins):Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 2Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 3Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 4Multi-Factor Authentication Market Worth 9.60 Billion USD by 2020 2Multi-Factor Authentication Market Worth 9.60 Billion USD by 2020 3Multi-Factor Authentication Market Worth 9.60 Billion USD by 2020 4Multi-Factor Authentication Market Worth 9.60 Billion USD by 2020 5Sarcos to Debut First-of-its-Kind Snake Robot 2Sarcos to Debut First-of-its-Kind Snake Robot 3
... Valladolid (Spain) has analysed 40 brands of beer, discovering that ... beers. Iron is essential to the human diet, but also ... and flavour. According to the analysis carried out by ... from all 5 continents, dark beers have an average free ...
... AUSTIN, Texas Just as the rainy season is driving ... computational model could forecast where outbreaks are likely to occur. ... Centers for Disease Control and Prevention (CDC) on the project, ... most needed in the earthquake-ravaged country. Just back from ...
... team of scientists has identified 29 new genetic variants ... biology of an important and very debilitating neurological disease. ... common neurological conditions among young adults, affects around 2.5 ... attacks the central nervous system (CNS), which includes the ...
Cached Biology News:Dark beer has more iron than pale beer 2Researchers fight cholera with computer forecasting 2Researchers fight cholera with computer forecasting 3Researchers fight cholera with computer forecasting 4Vanderbilt researchers, international team, uncover genes linked to multiple sclerosis 2
Red Standard is sodium salt of resorufin....
Human Jagged 2 Biotinylated Affinity Purified PAb...
RayBio L Series 507: RayBio Label-based Antibody Array I (2 membranes) Class: Antibody Array Products Product Group: Antibody Array...
The Zymo-Spin PI Fast-Spin column features durable polypropylene construction and is the same column featured in the His-Spin Protein Miniprep. Capacity is 800 l....
Biology Products: