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P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor

used liquid base to maintain pH at setpoint, relying on the acid-producing culture to lower pH if needed. The pH control parameters were:
Base . . . . . . . . . . . . .ammonium hydroxide, 30% solution
Pump . . . . . . . . . . . .Pump 1 of the 4-Pump Module
Transfer tubing . .. . .Silicone tubing, as supplied, P0740-2396.
Vessel inlet . . . . . . . .Triport adapter in the vessel headplate.

Control Setup:
1) Pump 1 plugged into "BASE" power-outlet of the Power Controller
2) pH Control Selections: Multiplier = 25%
Dead-band = 0
PID values: factory defaults


Results and Discussion
The DO and agitation trend graphs (Figures 1 & 2) reveal the fermentation history. We limited the carbon source in order to restrict growth to levels that non-oxygenenriched air could support, which resulted in a healthy culture. Temperature and pH were stable throughout the entire run.




The most important fermentor characteristics for highdensity cultures, such as Pichia , are the fermentor's maximum oxygen transfer rate (OTR) and maximum heat transfer rate. In dense robust cultures, the fermentor must:

1) incorporate oxygen at a high rate from the sparge gas into the dissolved oxygen needed for metabolism. Additionally, OTR depends on agitation-motor power and impeller design.

2) dissipate the heat of metabolism and agitation without allowing culture temperature to rise above the growth optimum. Good temperature control depends on cooling system design and coolant temperature.

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