Navigation Links
P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor

DO probe was calibrated at 0%, (obtained by briefly disconnecting the cable), and at 100% (obtained using 1,000 rpm agitation and 5 L/m (1 vvm) airflow. After calibration, DO remained at approximately 100% until inoculation.

An agitation cascade was selected in the controller to maintain DO at setpoint through automatic adjustment of agitation speed. The agitation cascade increases agitation speed with increasing oxygen demand. To set up the cascade, we used the DO control display and keypad on the PCU to select:
Cascade : . . . . . . . . . . . . . . . . . . . .Agit
Minimum RPM : . . . . . . . . . . . . . . .300
Maximum RPM: . . . . . . . . . . . . .1,200


Nutrient Feed
Initial feed was 360 mL of 50% glycerol solution with 12 mL/L of PTM1 (trace metals). BioCommand began this feed automatically when the dissolved oxygen showed a sudden rise above the setpoint, a well-known carbonexhaustion indicator. After all the glycerol was consumed, we allowed a brief starvation phase, then changed the feed to 100% methanol solution with 12 mL/L of PTM1 (trace metals) solution.

Glycerol . . . . . . . . . .Pump 2 of the 4-Pump Module
Methanol . . . . . . . . .Pump 3 of the 4-Pump Module
Transfer tubing . . . .Silicone tubing as supplied (1.4mm inner diameter and 4.8mm outer diameter), P0740-2396
Vessel inlet . . . . . . . Triport adapter in the vessel headplate

Control Setup:
1) Pump 2 plugged into "Pump A" power-outlet of the Power Controller.
2) Pump 3 plugged into "Pump B" power-outlet of the Power Controller.
3) Pumps A & B: Manual mode, controlled by BioCommand


PH Control
We
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. Fermentation Cell Culture: University Style
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
8. Outstanding transfection rates using the Multiporator
9. Outstanding transfection rates using the Multiporator
10. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
11. Normalizing cDNA libraries using the EppendorfThermomixer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: pastoris Fermentation using BioFlo Benchtop Fermentor

(Date:12/17/2014)... (PRWEB) December 17, 2014 Gene ... that it has entered into a technology access ... (ADM) to apply DNA2.0’s proprietary protein engineering technology, ... process. , “We are extremely excited that the ... platform. This proprietary bioengineering technology has now ...
(Date:12/17/2014)... SOUTH SAN FRANCISCO, Calif. , Dec. 17, ... positive results of a Phase 2 study evaluating ... for the treatment of patients with severe, chronic ... the current standard of care, including topical steroids ... endpoint was percent change in Visual Analog Scale ...
(Date:12/15/2014)...  GlassesOff Inc. (OTCBB: GLSO) announced today the appointment ... director of the Company,s Board of Directors. ... its CEO until its acquisition by Stanley Black ... Recognized as the inventor of the first Wi-Fi-based Active ... -based RFID solutions focused on improving operational efficiency, safety ...
(Date:12/13/2014)... (PRWEB) December 12, 2014 Clarassance, a ... announced its new name: Therabron Therapeutics , Inc. ... and bronchioles (a type of structure in the lungs ... company’s mission to develop novel protein therapeutics for the ... directors decided to change the name to mark the ...
Breaking Biology Technology:ADM and DNA2.0 Enter Into Protein Engineering Technology Access and Service Agreement 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 3Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 4AeroScout Founder Joins GlassesOff's Board of Directors 2AeroScout Founder Joins GlassesOff's Board of Directors 3Maryland-based Biotech Company's Path Forward in Treating Respiratory Diseases Sparks Name Change 2
... ... statistically significant pain relief at five minutes post-dose using electronic patient diary data ... PITTSBURGH, PA (Vocus) ... outcomes (PRO) solutions and services for global clinical research, today announced that its ...
... Ohio (March 29, 2010) Scientists have discovered the ... molecules less than one nanometer wide. The Ohio University-led ... the journal Nature Nanotechnology , provides the first ... which could be used for nanoscale electronic devices and ...
... ... , ... -- Today, Becky J. Anderson, For Every Body founder, announced the launch of the newest product, ... product will be showcased to the pet industry from Booth #916 at the Global Pet Expo ...
Cached Biology Technology:invivodata Delivers Critical Symptom Relief Data in INSYS Therapeutics' Breakthrough Cancer Pain Trial 2invivodata Delivers Critical Symptom Relief Data in INSYS Therapeutics' Breakthrough Cancer Pain Trial 3Scientists discover world's smallest superconductor 2Millions of Pet Owners to Benefit from Innovative Pet Odor Eliminator 2
(Date:11/18/2014)... 2014  The Secure Identity & Biometrics Association ... announce the formation of The Airport Entry and ... Identity and Biometric Entry and Exit Solutions Framework ... support from BORDERPOL, the international non-profit organization that ... expertise regarding border security, traveler and migration systems. ...
(Date:11/18/2014)... Nov. 18, 2014   EyeLock Inc. , ... and MorphoTrust USA (Safran), a leading ... announced a strategic partnership to offer comprehensive biometric ... administration (MVA), airport screening and financial services markets. ... MorphoTrust serves consumers through a nationwide network of ...
(Date:11/11/2014)... CHICAGO , Nov. 11, 2014  Forensicon, Inc., ... eDiscovery firm, is pleased to announce the promotion of ... to Director of Digital Forensics. In Schiff,s new role ... out the team of digital forensics examiners and provide ... Photo - http://photos.prnewswire.com/prnh/20141110/157719 ...
Breaking Biology News(10 mins):Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3EyeLock, MorphoTrust Form Strategic Partnership to Pursue Commercial Enterprise Deployments 2EyeLock, MorphoTrust Form Strategic Partnership to Pursue Commercial Enterprise Deployments 3Yaniv Schiff Promoted to Director of Digital Forensics at Forensicon, Inc. 2
... Calif., December 23, 2011 John C. Reed, M.D., Ph.D., ... Institute (Sanford-Burnham), have been named as Fellows of the American ... AAAS Fellow is an honor bestowed upon AAAS members by ... announced today in the AAAS News & Notes section of ...
... has long been known to strengthen the enamel on ... (HHMI) scientists finds that fluoride also has dramatic effects ... form plaque and can cause cavities. , HHMI researcher ... chain of events that occurs inside a bacterium after ...
... tragic losses to life, property, and the environment. But ... from done. Without vegetation, bare, burnt soil lies vulnerable ... regeneration. Now Assaf Inbar, a graduate student ... together with his supervisors Prof. Marcelo Sternberg of the ...
Cached Biology News:AAAS and Sanford-Burnham announce 2011 fellows 2Bacteria battle against toxic fluoride 2Bacteria battle against toxic fluoride 3Reclaiming the land after a forest fire 2
Normal sheep serum collected from healthy normal sheep...
Rabbit polyclonal to HMGB1 (rating: ****) ( Abpromise for all tested applications). entrezGeneID: 3146 SwissProtID: P09429...
Request Info...
... Highly efficient delivery of siRNA while ... Electroporate up to 96 samples simultaneously ... from cell harvest to post-electroporation incubation ... , Designed for use with ...
Biology Products: