Navigation Links
P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor

. . . . . .. .200 mL
Glycerol* . . . . . . . . . . . . . . . . . . . . . . . . . .< 400 mL
Methanol* . . . . . . . . . . . . . . . . . . . . . . . . .<2 L
Base (to maintain pH at setpoint)* . . . . .. . .< 250 mL
(*) Added, as required

Pichia trace metals solution, PTM1 consisted of :
Cupric sulfate pentahydrate . . . . . . . . . . . . . . .6 g/L
Sodium iodide . . . . . . . . . . . . . . . . . . . . . . . . .0.08 g/L
Manganese sulfate monohydrate . . . . . . . . .. . .3 g/L
Cobalt chloride (anhydrous) . . . . . . . . . . . .. . .0.5 g/L
Zinc chloride (anhydrous) . . . . . . . . . . . . . .. . .20 g/L
Boric acid . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.02 g/L
Sodium molybdate dihydrate . . . . . . . . . . . . . .0.2 g/L
Ferrous sulfate heptahydrate . . . . . . . . . . . . .. .65 g/L
Biotin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..0.2 g/L
6N sulfuric acid . . . . . . . . . . . . . . . . . . . . . . . .30 mL/L


Control Setpoints
Setpoints were keyed into the controller prior to inoculation and, except for DO which remained high until culture was introduced, the vessel was allowed to equilibrate prior to inoculation.
Temperature . . . . . . . . . . . . . . . . . . . . . . . 30C
pH . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . 5.0
Dissolved Oxygen . . . . . . . . . . . . . . . . ... . 30%
Agitation . . . . . . . . . . . . . . . . . . . . 300 - 1,200 rpm
(responding automatically to oxygen demand)


Dissolved Oxygen (DO) Control
The
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. Fermentation Cell Culture: University Style
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
8. Outstanding transfection rates using the Multiporator
9. Outstanding transfection rates using the Multiporator
10. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
11. Normalizing cDNA libraries using the EppendorfThermomixer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: pastoris Fermentation using BioFlo Benchtop Fermentor

(Date:5/28/2015)... , May 28, 2015 ... ) has announced the addition of ... - Technologies, Markets and Companies"  to ... ,This report describes and evaluates animal ... medicine and pharmaceuticals as well as ...
(Date:5/28/2015)... Research and Markets( http://www.researchandmarkets.com/research/l9dqwt/animal_genetics ) has announced the addition ... Testing services - Global Forecast to 2020" report ... market is estimated to be worth around $2.5 billion ... during the forecast period of 2014 to 2020 ... protein consumption, increasing adoption of advanced genetic technologies for ...
(Date:5/28/2015)... Research and Markets( http://www.researchandmarkets.com/research/3nl6s2/neuroprotection ) has ... "Neuroprotection - Drugs, Markets and Companies" ... the role of neuroprotection in acute disorders such ... as well as in chronic diseases such as ... of damage to neural tissues are similar in ...
(Date:5/28/2015)... 2015 Celsion Corporation (the "Company") (NASDAQ: ... from two institutional healthcare investors to purchase an aggregate ... in an at-the-market registered direct offering and a concurrent ... The Company entered into a definitive purchase agreement ... to sell an aggregate of 3,000,000 shares of its ...
Breaking Biology Technology:Global Animal Biotechnology Report 2015 - Technologies, Markets and Companies Analysis to 2024 2Global Animal Biotechnology Report 2015 - Technologies, Markets and Companies Analysis to 2024 3Global Animal Genetics (Canine, Equine, Poultry, Porcine, Bovine, and Genetic Material) Market 2015 - Global Forecast to 2020 2Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 2Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 3Celsion Corporation Announces $8 Million At-The-Market Registered Direct Offering 2Celsion Corporation Announces $8 Million At-The-Market Registered Direct Offering 3
... , EMERYVILLE, Calif., June 4 Ratcliff promoted Jorge Burbano ... Y. Keung to associate this month. The award-winning firm ... array of architectural, interior design and planning services to healthcare, ... , Jorge Burbano, LEED(R) AP, has more than 20 ...
... BRAINTREE, Mass., June 4 Haemonetics Corporation (NYSE: ... the District of Massachusetts has made a ruling in the ... 2009, a jury issued a verdict in favor of Haemonetics, ... U.S. Haemonetics patent. The jury awarded Haemonetics $15.7 million ...
... 11th, 10:00 a.m. EDT , , WALTHAM, Mass., June ... that the Company President and Chief Executive Officer, Walter C. Herlihy, ... 11th, 2009 at 10:00 a.m. EDT, to report fourth quarter and ... of the Company. , , This call is being webcast ...
Cached Biology Technology:Ratcliff Promotes Jorge Burbano to Senior Associate; Tess Kavanagh and Tony Keung to Associate 2Haemonetics Receives Favorable Ruling in Patent Lawsuit against Competitive Technology 2Repligen Announces Conference Call of Fourth Quarter and Fiscal Year 2009 Results 2Repligen Announces Conference Call of Fourth Quarter and Fiscal Year 2009 Results 3
(Date:5/19/2015)... May 18, 2015 Fingerprint Cards (FPC) ... FPC1025, FPC1145, FPC1155 and FPC1035 from one if its module ... and including Q3 2015 and the sensors will be used ... order values for 2015 hereby amount to 740 MSEK to ... of 140 MSEK and a number of smaller orders not ...
(Date:5/14/2015)... , May 14, 2015 Research and Markets ... the "Fingerprint Sensors Market in Smart Mobile Devices ... report believes that 2014 was a watershed year for ... introduction of ,Apple Pay,. Apple gave fingerprint sensors a ... payment service. Fingerprint sensors are a must-have feature in ...
(Date:5/11/2015)... 11, 2015 Curemark LLC, a privately held ... a new Phase III double blind, randomized, placebo-controlled clinical ... CM-AT, on all children ages 3-8 with Autism. Previously, ... III double blinded clinical trial for CM-AT in children ... the digestive enzyme chymotrypsin. This new trial will help ...
Breaking Biology News(10 mins):Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Global Smart Mobile Devices Fingerprint Sensors Market 2015-2019: Key Vendors are Authentec, Validity Sensors, Fingerprint Cards, Next Biometrics Group. IDEX, and Qualcomm 2Curemark, LLC, Launches New Phase III Trial in Expanded Population of Children with Autism 2
... of Basic Medical Sciences, Beijing University of Chinese ... advanced glycation end product formation, reduce their levels ... memory capabilities in ovariectomized rats. The Radix Achyranthis ... accumulation is associated with an antioxidative effect. These ...
... water systems is the focus of a wide-ranging collection ... the Elsevier journal Environmental Development . A ... the UN University,s Canadian-based Institute for Water, Environment and ... articles available with open public access until the end ...
... data obtained during six years of regular aerial surveys and ... have strengthened evidence pointing to the central Gulf of Maine ... to a study recently published online in the journal ... ( Eubalaena glacialis ) is one of the most endangered ...
Cached Biology News:Managing waters shared across national boundaries: Treasury of papers helps capture 20 years of lessons 2Managing waters shared across national boundaries: Treasury of papers helps capture 20 years of lessons 3Managing waters shared across national boundaries: Treasury of papers helps capture 20 years of lessons 4Managing waters shared across national boundaries: Treasury of papers helps capture 20 years of lessons 5Are North Atlantic right whales mating in the Gulf of Maine? 2Are North Atlantic right whales mating in the Gulf of Maine? 3
... Immunoperoxidase Secondary Detection System uses biotin-avidin-HRP complexed ... in the Primary Antibody. The primary antibody ... specimen. Any excess antibody is removed by ... to biotin) is added and will react ...
... double stranded RNA (dsRNA) has been shown ... group of organisms as well as insect ... the inhibition of protein expression by utilizing ... RNA (mRNA). Attempts to induce RNA interference ...
... The TSQ Quantum Ultra defines a new ... environmental analysis. It features the revolutionary ... a titanium skimmer with superior skimming and ... Its redesigned ion transfer tube lens ...
... tetramethylrhodamine-conjugated jetSI-ENDO (excitation at 555 ... the powerful siRNA transfection reagent. ... determine the transfection efficiency or ... trafficking of siRNAs. More than ...
Biology Products: