Navigation Links
P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor

inal 5.7 L working volume. All BioFlo 110 fermentation vessels are configured with a 4-baffle stainless-steel insert, dual Rushton agitation impellers, and a high-speed, direct-drive agitation system with mechanical face-seal. Dissolved oxygen and pH probes (Mettler Toledo) are also included, as are a variety of items such as liquid addition bottle kits (3), cables, tubing and clamps.


Control System
The four control modules included with the Advanced Fermentation Kit were used for the first run.



Materials and Methods
Overview

This Pichia fermentation follows a well-established protocol in which glycerol is the initial carbon source, and after a brief carbon starvation, we switch to a methanol feed. The switch to methanol produces a metabolite of interest by triggering the AOX1 promoter in genetically engineered Pichia . These are fed-batch fermentations, since first glycerol and later methanol is added while the culture is growing.


Control Program
We created a feed control program using BioCommand Plus software, NBS Catalog Number M1291-0000. It turned on the glycerol feed-pump when the dissolved oxygen (DO) level rose above 40%. Each time DO exceeded 40%, the glycerol pump turned on; each time it fell below 40%, the pump turned off. 40% is a high DO level, indicative of reduced metabolism due to carbon exhaustion. The rationale for this strategy is that the DO increases due to reduced growth of the cells, which is a result of nutrient depletion. Approximately one hour after a rise in DO which indicated depletion of the supplementary glycerol, the program automatically turned on the methanol feed-pump.


Inoculum
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. Fermentation Cell Culture: University Style
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
8. Outstanding transfection rates using the Multiporator
9. Outstanding transfection rates using the Multiporator
10. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
11. Normalizing cDNA libraries using the EppendorfThermomixer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: pastoris Fermentation using BioFlo Benchtop Fermentor

(Date:1/15/2014)... The Microcompetition with Foreign DNA theory explains how latent viruses ... is the Epstein Barr Virus (EBV), and one of these ... chronic inflammatory disease that destroys the body’s joints. As predicted ... high concentrations of EBV DNA in their bodies (1). ...
(Date:1/15/2014)... 2013 was a banner year of continued innovation ... continued independent research led by the team at Wake ... million grant from the Susanne Marcus Collins Foundation, had ... reviewed journal, Amy Grant highlighted Brainwave Optimization® in First ...
(Date:1/15/2014)... AudioNotch is the internet's leading provider of ... of tinnitus. Patients listen to sound therapy that is ... of weeks to months, their tinnitus volume decreases. , AudioNotch ... Music and Notched White Noise. Now, AudioNotch is pleased to ...
(Date:1/14/2014)... Date: Friday, April 11, 2014 , Time: 6 ... Almshouse Road, Warrington, Pa. , Details: The Hepatitis ... finding a cure for hepatitis B and improving the quality ... Crystal Ball on Friday, April 11 at Warrington Country Club ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2
... Va. Developing a credit-card-sized gas chromatography platform ... the next step for Virginia Tech College of ... National Science Foundation (NSF) Faculty Early Career Development ... Agah, an assistant professor in the Bradley Department ...
... Edwards Lifesciences,Corporation (NYSE: EW ), a ... cardiovascular disease, today announced the company,s,strengthened alignment into ... departure in the middle of 2008 of corporate ... Martin., "To support our growth strategy, we ...
... Md., Feb. 18 - Biotech pioneer,William J. Rutter, ... 2008 RAPS,Horizons Conference & Exhibition in San Francisco ... announced., Rutter, Chairman and CEO of Synergenics ... stimulate fresh and relevant perspectives on,the regulatory implications ...
Cached Biology Technology:Credit-card-sized platform for volatile compound analysis CAREER project goal 2Edwards Lifesciences Announces Organizational Changes 2Biotech Pioneer William J. Rutter to Deliver Keynote at 2008 RAPS Horizons Conference 2
(Date:4/17/2014)... , Respiratory syncytial virus (RSV) is a ... life-threating illness in very young and elderly populations. ... enters host cells through the fusion protein RSV ... interfering peptides that prevent bundle formation limit RSV ... susceptible to degradation. In this issue of the ...
(Date:4/17/2014)... 16, 2014. Kessler Foundation has been named awardee ... the Department of Defense Spinal Cord Injury Research ... for the randomized, double-blinded, controlled, multi-site clinical trial, ... muscle strength after spinal cord injury. Dr. Forrest ... Research at Kessler Foundation. Two additional sites will ...
(Date:4/17/2014)... cartridge developed at Sandia National Laboratories and recently licensed ... and cheaper. , Bacillus anthracis , the ... all over the world and can cause serious, and ... bacteria can survive in harsh conditions for decades. In ... through skin contact, inhalation of spores or eating contaminated ...
Breaking Biology News(10 mins):JCI online ahead of print table of contents for April 17, 2014 2JCI online ahead of print table of contents for April 17, 2014 3JCI online ahead of print table of contents for April 17, 2014 4JCI online ahead of print table of contents for April 17, 2014 5JCI online ahead of print table of contents for April 17, 2014 6Kessler Foundation awarded Department of Defense grant for spinal cord injury research 2Pocket-sized anthrax detector aids global agriculture 2Pocket-sized anthrax detector aids global agriculture 3
... key roles in nature and are valued for their great importance ... and pharmaceuticals produced on a large-scale basis for decades with the ... A. niger is an integral player in the carbon cycle, ... breaking down plant cell walls to free up sugars that can ...
... cars produced worldwide in 2010 surpassed the 70 million mark. Europe ... the number of new cars and drivers on the road increasing ... imperative. Over 40,000 lives are lost in the EU in ... 1.7 million citizens injured in such incidents. The European Commission has ...
... photo of a cornfield and a big photovoltaic array," says ... in St. Louis. "When I give talks I often ask ... votes overwhelmingly in favor of photosynthesis. " They are ... the point of departure for a provocative article published in ...
Cached Biology News:Same fungus, different strains 2Same fungus, different strains 3Driving changes in automotive safety 2Driving changes in automotive safety 3Driving changes in automotive safety 4Turning plants into power houses 2Turning plants into power houses 3Turning plants into power houses 4Turning plants into power houses 5Turning plants into power houses 6
... concentrator for efficient drying of small volumes ... system combines the SpeedVac™ Concentrator and chemically ... pump and rotor control, and dual timers ... time), makes the DNA120 indispensable in the ...
... kit. Includes configured Laptop PC with PCR ... MultiBlock satellite thermal Cycler (non-robotic). System ... software. Store/track run data. Gradient ... Outstanding uniformity. Advanced control algorithms for ...
... Px2 Thermal Cycler is Thermo Electron's most ... date with easy to use sophisticated onboard ... easy programming. Designed to offer performance, accuracy ... incorporates a number of customer driven features ...
0.2ml Gradient Block MBSR. Robot compatible block ejection mechanism. Stackable. (96 well block - holds 1 plate 0.2ml x 96 well plate)...
Biology Products: