Navigation Links
Outstanding transfection rates using the Multiporator

Using eukaryotic cells, extremely high transfection rates can be achieved due to a hypoosmolar buffer system and short pulses in the s-range. High reproducibility is ensured by an electronically controlled pulse, independent of sample resistance (patent pending). Experimental data
Two cell lines (mouse fibroblast cells, human T-lymphocytes) were transfected using plasmid-DNA and two different electroporation systems, the new Eppendorf Multiporator (A) and a system of a well-known competitor (B).

Transfections were performed according to the manufacturers protocols. Transfection rates were calculated using FACS analysis.

Transfection rates after 48 h in relation to initial number of cells
Multiporator (A) Competitor (B)
Mouse fibroblast cells 54.25%
22.16%
n=8
2.03%
1.12%
n=8
Human T-lymphocytes 29.92%
9.26%
n=10
0.55%
0.6%
n=10




'"/>

Source:


Page: All 1

Related biology technology :

1. Outstanding transfection rates using the Multiporator
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
4. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
5. siRNA transfection optimization with the Agilent 2100 bioanalyzer
6. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
7. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
8. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
9. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
10. Normalizing cDNA libraries using the EppendorfThermomixer
11. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/8/2016)... Feb. 8, 2016  BioElectronics Corporation (OTC Pink: ... announced today that it is responding to a ... the Securities and Exchange Commission posted on the ... Chairman of the Board of BioElectronics Corporation and ... Administration at The Fuqua School of Business, Duke ...
(Date:2/8/2016)... ... February 08, 2016 , ... ... of Semantic Graph Database technology, today announced the availability of AllegroGraph 6, the ... through the Cloudera Certified Technology Program (CCPT). AllegroGraph is the first ...
(Date:2/8/2016)... Mich. , Feb. 8, 2016  Diplomat Pharmacy, Inc. (NYSE: DPLO) announced today that its ... a specialty pharmacy resource–user-centric, story-driven, knowledge-based and mobile-friendly. ... ... ... "The goal ...
(Date:2/6/2016)... ... February 06, 2016 , ... Contact:, ... Center for Excellence in Education Sponsors Teacher Training Program , Bite of Science ... – The Center for Excellence in Education (CEE) will sponsor a Bite of ...
Breaking Biology Technology:
(Date:1/22/2016)... DUBLIN , Jan. 22, 2016 /PRNewswire/ ... announced the addition of the "Global ... report to their offering. --> ... of the "Global Biometrics Market in ... offering. --> Research and ...
(Date:1/20/2016)... --  MedNet Solutions , an innovative SaaS-based eClinical technology ... is pleased to announce the attainment of record-setting corporate ... of the company,s laser focus on (and growing international ... comprehensive, easy-to-use and highly affordable cloud-based technology platform. ... growth achievements in 2015 include: , Record ...
(Date:1/15/2016)... Rico , Jan. 15, 2016 Recent ... and small to find new ways to ensure data ... iOS and Android that ... on biometrics, transforming it into a hardware authorization token. ... users swipe their fingerprint on their KodeKey enabled device ...
Breaking Biology News(10 mins):