Navigation Links
Outstanding transfection rates using the Multiporator

Using eukaryotic cells, extremely high transfection rates can be achieved due to a hypoosmolar buffer system and short pulses in the s-range. High reproducibility is ensured by an electronically controlled pulse, independent of sample resistance (patent pending). Experimental data
Two cell lines (mouse fibroblast cells, human T-lymphocytes) were transfected using plasmid-DNA and two different electroporation systems, the new Eppendorf Multiporator (A) and a system of a well-known competitor (B).

Transfections were performed according to the manufacturers protocols. Transfection rates were calculated using FACS analysis.

Transfection rates after 48 h in relation to initial number of cells
Multiporator (A) Competitor (B)
Mouse fibroblast cells 54.25%
22.16%
n=8
2.03%
1.12%
n=8
Human T-lymphocytes 29.92%
9.26%
n=10
0.55%
0.6%
n=10




'"/>

Source:


Page: All 1

Related biology technology :

1. Outstanding transfection rates using the Multiporator
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
4. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
5. siRNA transfection optimization with the Agilent 2100 bioanalyzer
6. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
7. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
8. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
9. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
10. Normalizing cDNA libraries using the EppendorfThermomixer
11. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/8/2016)... England , December 8, 2016 ... has expanded its customisable SureSeq™ NGS panel range with the ... allowing fast and cost-effective study of variants in familial hypercholesterolemia ... number variation (CNV) detection on a single small panel and ... hotspot content. This includes all exons for LDLR , ...
(Date:12/8/2016)... ... December 08, 2016 , ... KBioBox llc ... to client demand KbioBox developed a sophisticated “3 click” gene dditing off target ... from KBioBox’s new website, https://www.kbiobox.com/ and powered by the company’s ...
(Date:12/8/2016)... ... December 08, 2016 , ... Microbial genomics ... Awards. uBiome is one of just six company finalists in the Health & ... to uBiome, companies nominated as finalists in this year’s awards include Google, SpaceX, ...
(Date:12/8/2016)...  Soligenix, Inc. (OTCQB: SNGX) (Soligenix or the ... and commercializing products to treat rare diseases where ... the long-term follow-up data from its Phase 2 ... Defense Regulator (IDR), in the treatment of oral ... undergoing chemoradiation therapy (CRT).  The additional 12-month safety ...
Breaking Biology Technology:
(Date:12/7/2016)... 7, 2016   Avanade is helping Williams ... teams in history, exploit biometric data in order to ... maintain the competitive edge against their rivals after their ... Avanade has worked with Williams during the 2016 ... data (heart rate, breathing rate, temperature and peak acceleration) ...
(Date:12/6/2016)... Securus Technologies, a leading provider of ... safety, investigation, corrections and monitoring, and the Prison ... (5) year funding commitment by Securus to PEP ... and reentry support to more inmates and their ... the Prison Entrepreneurship Program (PEP) is an independent ...
(Date:11/30/2016)... Nov. 30, 2016 Not many of us realize that we spend ? ... so we need to do it well. Inadequate sleep levels have been found to ... stroke, diabetes, and even cancer. Maybe now is the best time to ... help them to manage their sleep quality? Continue Reading ... ...
Breaking Biology News(10 mins):