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Outstanding transfection rates using the Multiporator

Using eukaryotic cells, extremely high transfection rates can be achieved due to a hypoosmolar buffer system and short pulses in the s-range. High reproducibility is ensured by an electronically controlled pulse, independent of sample resistance (patent pending). Experimental data
Two cell lines (mouse fibroblast cells, human T-lymphocytes) were transfected using plasmid-DNA and two different electroporation systems, the new Eppendorf Multiporator (A) and a system of a well-known competitor (B).

Transfections were performed according to the manufacturers protocols. Transfection rates were calculated using FACS analysis.

Transfection rates after 48 h in relation to initial number of cells
Multiporator (A) Competitor (B)
Mouse fibroblast cells 54.25%
22.16%
n=8
2.03%
1.12%
n=8
Human T-lymphocytes 29.92%
9.26%
n=10
0.55%
0.6%
n=10




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