Navigation Links
Outstanding transfection rates using the Multiporator

Using eukaryotic cells, extremely high transfection rates can be achieved due to a hypoosmolar buffer system and short pulses in the s-range. High reproducibility is ensured by an electronically controlled pulse, independent of sample resistance (patent pending). Experimental data
Two cell lines (mouse fibroblast cells, human T-lymphocytes) were transfected using plasmid-DNA and two different electroporation systems, the new Eppendorf Multiporator (A) and a system of a well-known competitor (B).

Transfections were performed according to the manufacturers protocols. Transfection rates were calculated using FACS analysis.

Transfection rates after 48 h in relation to initial number of cells
Multiporator (A) Competitor (B)
Mouse fibroblast cells 54.25%
22.16%
n=8
2.03%
1.12%
n=8
Human T-lymphocytes 29.92%
9.26%
n=10
0.55%
0.6%
n=10




'"/>

Source:


Page: All 1

Related biology technology :

1. Outstanding transfection rates using the Multiporator
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
4. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
5. siRNA transfection optimization with the Agilent 2100 bioanalyzer
6. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
7. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
8. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
9. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
10. Normalizing cDNA libraries using the EppendorfThermomixer
11. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:9/18/2017)... , Sept. 18, 2017   Montrium , a growing leader ... exciting new partnership with a groundbreaking non-profit research organization, Multidisciplinary ... ... psychotherapy studies for PTSD ... recently granted Breakthrough Therapy Designation to MDMA for the treatment of ...
(Date:9/14/2017)... ... September 14, 2017 , ... ... scientist program (FSP)-- a flexible business approach similar to a full-time equivalent ... extensive project-based analytical and solid-state chemistry services and expertise with flexible extended ...
(Date:9/14/2017)... Australia (PRWEB) , ... September 14, 2017 , ... ... first viscoelastic Freedom Lumbar Disc case in Australia. Dr. Steven Yang completed the ... pain as a result of a degenerative lumbar disc at level L5-S1. The ...
(Date:9/13/2017)... , ... September 13, 2017 , ... ... the life sciences industry to improve patient outcomes and quality of life for ... impurities, has been named a US expert to the International Standards Organization/Technical ...
Breaking Biology Technology:
(Date:3/29/2017)... the health IT company that operates the largest health ... today announced a Series B investment from BlueCross BlueShield ... investment and acquisition accelerates higi,s strategy to create the ... activities through the collection and workflow integration of ambient ... secures data today on behalf of over 36 million ...
(Date:3/27/2017)... 2017  Catholic Health Services (CHS) has been ... (HIMSS) Analytics for achieving Stage 6 on the ... In addition, CHS previously earned a place in ... electronic medical record (EMR). "HIMSS Analytics ... EMR usage in an outpatient setting.  This recognition ...
(Date:3/24/2017)... Mar 24, 2017 Research and Markets has ... Market Analysis & Trends - Industry Forecast to 2025" report ... ... at a CAGR of around 15.1% over the next decade to ... report analyzes the market estimates and forecasts for all the given ...
Breaking Biology News(10 mins):